Cited by 328Cancer, Hypoxia, and MetabolismCancer, Lipids, and MetabolismCancer and biochemical research

The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.

Comparative Metabolic Flux Profiling of Melanoma Cell Lines

Open access

Scott, David A., Richardson, Adam D., Filipp, Fabian V., Knutzen, Christine A., Chiang, Gary G., Ronai, Ze'ev A., Osterman, Andrei L., Smith, Jeffrey W.·2011·Journal of Biological Chemistry

DOI 10.1074/jbc.m111.282046PMC PMC3234981

Open this paper in CoChat — free account Open PDF

Abstract

Metabolic rewiring is an established hallmark of cancer, but the details of this rewiring at a systems level are not well characterized. Here we acquire this insight in a melanoma cell line panel by tracking metabolic flux using isotopically labeled nutrients. Metabolic profiling and flux balance analysis were used to compare normal melanocytes to melanoma cell lines in both normoxic and hypoxic conditions. All melanoma cells exhibited the Warburg phenomenon; they used more glucose and produced more lactate than melanocytes. Other changes were observed in melanoma cells that are not described by the Warburg phenomenon. Hypoxic conditions increased fermentation of glucose to lactate in both melanocytes and melanoma cells (the Pasteur effect). However, metabolism was not strictly glycolytic, as the tricarboxylic acid (TCA) cycle was functional in all melanoma lines, even under hypoxia. Furthermore, glutamine was also a key nutrient providing a substantial anaplerotic contribution to the TCA cycle. In the WM35 melanoma line glutamine was metabolized in the "reverse" (reductive) direction in the TCA cycle, particularly under hypoxia. This reverse flux allowed the melanoma cells to synthesize fatty acids from glutamine while glucose was primarily converted to lactate. Altogether, this study, which is the first comprehensive comparative analysis of metabolism in melanoma cells, provides a foundation for targeting metabolism for therapeutic benefit in melanoma.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1074/jbc.m111.282046

Cites

44 of 44 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Understanding the Warburg Effect: The Metabolic Requirements of Cell Proliferation
Vander Heiden, M. G., Cantley, L. C., & Thompson, C. B. (2009) · Science · doi:10.1126/science.1160809 · Cited by 16,197
Cluster analysis and display of genome-wide expression patterns
Eisen, M. B., Spellman, P. T., Brown, P. O., & Botstein, D. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.25.14863 · Cited by 16,184
HIF-1-mediated expression of pyruvate dehydrogenase kinase: A metabolic switch required for cellular adaptation to hypoxia
Kim, J.-W., Tchernyshyov, I., Semenza, G. L., & Dang, C. V. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.02.002 · Cited by 3,905
On Respiratory Impairment in Cancer Cells
Warburg, O. H. (1956) · Science · doi:10.1126/science.124.3215.269 · Cited by 3,472
Otto Warburg's contributions to current concepts of cancer metabolism
Koppenol, W. H., Bounds, P. L., & Dang, C. V. (2011) · Nature Reviews Cancer · doi:10.1038/nrc3038 · Cited by 3,427
Java Treeview—extensible visualization of microarray data
Saldanha, A. J. (2004) · Bioinformatics · doi:10.1093/bioinformatics/bth349 · Cited by 2,991
Beyond aerobic glycolysis: transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis
DeBerardinis, R. J., Mancuso, A., Daikhin, E., Nissim, I., Yudkoff, M., Wehrli, S., & Thompson, C. B. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709747104 · Cited by 2,622
HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumption
Papandreou, I., Cairns, R. A., Fontana, L., Lim, A. L., & Denko, N. C. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.01.012 · Cited by 2,267
GeneCards Version 3: the human gene integrator
Safran, M., Dalah, I., Alexander, J., Rosen, N., Stein, T. I., Shmoish, M., Nativ, N., Bahir, I., Doniger, T., Krug, H., et al. (2010) · Database · doi:10.1093/database/baq020 · Cited by 2,134
The Common Feature of Leukemia-Associated IDH1 and IDH2 Mutations Is a Neomorphic Enzyme Activity Converting α-Ketoglutarate to 2-Hydroxyglutarate
Ward, P. S., Patel, J., Wise, D. R., Abdel-Wahab, O., Bennett, B. D., Coller, H. A., Cross, J. R., Fantin, V. R., Hedvat, C. V., Perl, A. E., et al. (2010) · Cancer Cell · doi:10.1016/j.ccr.2010.01.020 · Cited by 2,020

Show all 44 references

Research works printed but not linked: 4

Russo, 2009, Int. J. Oncol., 34, 1481
Yang, 2007, Pac. Symp. Biocomput., 181
Shah, 2010, PLoS Genet., 6
Eng, 2010, Sci. Signal, 3, ra31

Sorted by citations

Glutamine and cancer: cell biology, physiology, and clinical opportunities
Hensley, C. T., Wasti, A. T., & DeBerardinis, R. J. (2013) · Journal of Clinical Investigation · doi:10.1172/jci69600 · Cited by 1,262
ERK signalling: A master regulator of cell behaviour, life and fate
Lavoie, H., Gagnon, J., & Therrien, M. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0255-7 · Cited by 1,185
Energy metabolism of cancer: Glycolysis versus oxidative phosphorylation (Review)
Zheng, J. (2012) · Oncology Letters · doi:10.3892/ol.2012.928 · Cited by 986
Cellular Metabolism and Disease: What Do Metabolic Outliers Teach Us?
DeBerardinis, R. J., & Thompson, C. B. (2012) · Cell · doi:10.1016/j.cell.2012.02.032 · Cited by 858
Glutamine oxidation maintains the TCA cycle and cell survival during impaired mitochondrial pyruvate transport
Yang, C., Ko, B., Hensley, C. T., Jiang, L., Wasti, A. T., Kim, J., Sudderth, J., Calvaruso, M. A., Lumata, L., Mitsche, M., et al. (2014) · Molecular Cell · doi:10.1016/j.molcel.2014.09.025 · Cited by 679
Metabolic reprogramming during neuronal differentiation from aerobic glycolysis to neuronal oxidative phosphorylation
Zheng, X., Boyer, L., Jin, M., Mertens, J., Kim, Y., Ma, L., Ma, L., Hamm, M., Gage, F. H., & Hunter, T. (2016) · eLife · doi:10.7554/elife.13374 · Cited by 671
“More details including the parameters of machine settings can be found in the publication from the center (Scott et al., 2011).”
Background
Overcoming Intrinsic Multidrug Resistance in Melanoma by Blocking the Mitochondrial Respiratory Chain of Slow-Cycling JARID1Bhigh Cells
Roesch, A., Vultur, A., Bogeski, I., Wang, H., Zimmermann, K. M., Speicher, D., Körbel, C., Laschke, M. W., Gimotty, P. A., Philipp, S. E., et al. (2013) · Cancer Cell · doi:10.1016/j.ccr.2013.05.003 · Cited by 670
RAF-MEK-ERK pathway in cancer evolution and treatment
Ullah, R., Yin, Q., Snell, A. H., & Wan, L. (2022) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2021.05.010 · Cited by 553
Glutamine synthetase activity fuels nucleotide biosynthesis and supports growth of glutamine-restricted glioblastoma
Tardito, S., Oudin, A., Ahmed, S. U., Fack, F., Keunen, O., Zheng, L., Miletic, H., Sakariassen, P. Ø., Weinstock, A., Wagner, A., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3272 · Cited by 557
Altered metabolite levels in cancer: implications for tumour biology and cancer therapy
Sullivan, L. B., Gui, D. Y., & Heiden, M. G. V. (2016) · Nature Reviews Cancer · doi:10.1038/nrc.2016.85 · Cited by 406
Mitochondria as biosynthetic factories for cancer proliferation
Ahn, C. S., & Metallo, C. M. (2015) · Cancer & Metabolism · doi:10.1186/s40170-015-0128-2 · Cited by 399
Hypoxia-Mediated Increases in l -2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress
Oldham, W. M., Clish, C. B., Yang, Y., & Loscalzo, J. (2015) · Cell Metabolism · doi:10.1016/j.cmet.2015.06.021 · Cited by 364
“Given that PASMC proliferate in response to hypoxia, one interesting hypothesis is that the D2HG represents incomplete reductive carboxylation by IDH in these cells as has been observed in a variety of cancer cell lines (Gameiro et al., 2013; Scott et al., 2011; Wise et al., 2011).”
Influential
Metabolic reprogramming in cancer: Unraveling the role of glutamine in tumorigenesis
Daye, D., & Wellen, K. E. (2012) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2012.02.002 · Cited by 387
Understanding metabolic regulation and its influence on cell physiology
Metallo, C. M., & Vander Heiden, M. G. (2013) · Molecular Cell · doi:10.1016/j.molcel.2013.01.018 · Cited by 367
Dysregulated metabolism contributes to oncogenesis
Hirschey, M. D., DeBerardinis, R. J., Diehl, A. M., Drew, J. E., Frezza, C., Green, M. F., Jones, L. W., Ko, Y. H., Le, A., Lea, M. A., et al. (2015) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2015.10.002 · Cited by 316
Hypoxia, cancer metabolism and the therapeutic benefit of targeting lactate/H(+) symporters
Marchiq, I., & Pouysségur, J. (2016) · Journal of Molecular Medicine · doi:10.1007/s00109-015-1307-x · Cited by 300
The PI3K/Akt Pathway and Glucose Metabolism: A Dangerous Liaison in Cancer
Fontana, F., Giannitti, G., Marchesi, S., & Limonta, P. (2024) · International Journal of Biological Sciences · doi:10.7150/ijbs.89942 · Cited by 309
IDH1 mutations alter citric acid cycle metabolism and increase dependence on oxidative mitochondrial metabolism
Grassian, A. R., Parker, S. J., Davidson, S. M., Divakaruni, A. S., Green, C. R., Zhang, X., Slocum, K. L., Pu, M., Lin, F., Vickers, C., et al. (2014) · Cancer Research · doi:10.1158/0008-5472.can-14-0772-t · Cited by 280
Regulation of Substrate Utilization by the Mitochondrial Pyruvate Carrier
Vacanti, N. M., Divakaruni, A. S., Green, C. R., Parker, S. J., Henry, R. R., Ciaraldi, T. P., Murphy, A. N., & Metallo, C. M. (2014) · Molecular Cell · doi:10.1016/j.molcel.2014.09.024 · Cited by 277
Aerobic glycolysis and high level of lactate in cancer metabolism and microenvironment
Jiang, B. (2017) · Genes & Diseases · doi:10.1016/j.gendis.2017.02.003 · Cited by 277
A review of the basics of mitochondrial bioenergetics, metabolism, and related signaling pathways in cancer cells: Therapeutic targeting of tumor mitochondria with lipophilic cationic compounds
Kalyanaraman, B., Cheng, G., Hardy, M., Ouari, O., López, M., Joseph, J., Zielonka, J., & Dwinell, M. B. (2017) · Redox Biology · doi:10.1016/j.redox.2017.09.020 · Cited by 239
The cancer driver genes IDH1/2, JARID1C/ KDM5C, and UTX/ KDM6A: crosstalk between histone demethylation and hypoxic reprogramming in cancer metabolism
Chang, S., Yim, S., & Park, H. (2019) · Experimental & Molecular Medicine · doi:10.1038/s12276-019-0230-6 · Cited by 228
Role of abnormal lipid metabolism in development, progression, diagnosis and therapy of pancreatic cancer
Świerczyński, J. (2014) · World Journal of Gastroenterology · doi:10.3748/wjg.v20.i9.2279 · Cited by 203
Metabolic strategies of melanoma cells: Mechanisms, interactions with the tumor microenvironment, and therapeutic implications
Fischer, G. M., Gopal, Y. N. V., McQuade, J. L., Peng, W., DeBerardinis, R. J., & Davies, M. A. (2017) · Pigment Cell & Melanoma Research · doi:10.1111/pcmr.12661 · Cited by 200
Suppression of PGC-1α Is Critical for Reprogramming Oxidative Metabolism in Renal Cell Carcinoma
LaGory, E. L., Wu, C., Taniguchi, C. M., Ding, C. C., Chi, J., von Eyben, R., Scott, D. A., Richardson, A. D., & Giaccia, A. J. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.06.006 · Cited by 198
Self-citation
Interruption of lactate uptake by inhibiting mitochondrial pyruvate transport unravels direct antitumor and radiosensitizing effects
Corbet, C., Bastien, E., Draoui, N., Doix, B., Mignion, L., Jordan, B. F., Marchand, A., Vanherck, J., Chaltin, P., Schakman, O., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-03525-0 · Cited by 189
Dysfunctional oxidative phosphorylation makes malignant melanoma cells addicted to glycolysis driven by the V600EBRAF oncogene
Hall, A., Meyle, K. D., Lange, M. K., Klima, M., Sanderhoff, M., Dahl, C., Abildgaard, C., Thorup, K., Moghimi, S. M., Jensen, P. B., et al. (2013) · Oncotarget · doi:10.18632/oncotarget.965 · Cited by 191
Reverse TCA cycle flux through isocitrate dehydrogenases 1 and 2 is required for lipogenesis in hypoxic melanoma cells
Filipp, F. V., Scott, D. A., Ronai, Z. A., Osterman, A. L., & Smith, J. W. (2012) · Pigment Cell & Melanoma Research · doi:10.1111/j.1755-148x.2012.00989.x · Cited by 193
Self-citation
Resistance to BRAF inhibitors induces glutamine dependency in melanoma cells
Baenke, F., Chaneton, B., Smith, M., van den Broek, N. J. F., Hogan, K., Tang, H., Virós, A., Martin, M. J., Galbraith, L. C. A., Girotti, M. R., et al. (2015) · Molecular Oncology · doi:10.1016/j.molonc.2015.08.003 · Cited by 191
Functional Specialization in Proline Biosynthesis of Melanoma
Ingeniis, J. D., Ratnikov, B. I., Richardson, A. D., Scott, D. A., Aza‐Blanc, P., De, S. K., Kazanov, M. D., Pellecchia, M., Ronai, Z. A., Osterman, A. L., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0045190 · Cited by 186
Self-citation
Mitochondrial oxidative stress is the achille's heel of melanoma cells resistant to Braf-mutant inhibitor
Corazao-Rozas, P., Guerreschi, P., Jendoubi, M., André, F., Jonneaux, A., Scalbert, C., Garçon, G., Malet‐Martino, M., Balayssac, S., Rocchi, S., et al. (2013) · Oncotarget · doi:10.18632/oncotarget.1420 · Cited by 188
Metabolic Heterogeneity of Cancer Cells: An Interplay between HIF-1, GLUTs, and AMPK
Moldogazieva, N. T., Mokhosoev, I. M., & Terentiev, A. A. (2020) · Cancers · doi:10.3390/cancers12040862 · Cited by 181
Lessons from patient-derived xenografts for better in vitro modeling of human cancer
Choi, S. Y. C., Lin, D., Gout, P. W., Collins, C. C., Xu, Y., & Wang, Y. Z. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.09.009 · Cited by 181
Pharmacological Inhibition of Nicotinamide Phosphoribosyltransferase (NAMPT), an Enzyme Essential for NAD+ Biosynthesis, in Human Cancer Cells
Tan, B., Young, D. A., Lu, Z.-H., Wang, T., Meier, T. I., Shepard, R. L., Roth, K. D. W., Zhai, Y. W., Huss, K., Kuo, M., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.394510 · Cited by 181
Importance of glycolysis and oxidative phosphorylation in advanced melanoma
Ho, J., de Moura, M. B., Lin, Y., Vincent, G., Thorne, S. H., Duncan, L. M., Lin, H., Kirkwood, J. M., Becker, D., Houten, B. V., et al. (2012) · Molecular Cancer · doi:10.1186/1476-4598-11-76 · Cited by 173
Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides
Liu, W., Hancock, C. N., Fischer, J. W., Harman, M., & Phang, J. M. (2015) · Scientific Reports · doi:10.1038/srep17206 · Cited by 175
Metabolic rewiring in melanoma
Ratnikov, B. I., Scott, D. A., Osterman, A. L., Smith, J. W., & Ronai, Z. A. (2016) · Oncogene · doi:10.1038/onc.2016.198 · Cited by 176
Self-citation
Oncogene-Directed Alterations in Cancer Cell Metabolism
Nagarajan, A., Malvi, P., & Wajapeyee, N. (2016) · Trends in Cancer · doi:10.1016/j.trecan.2016.06.002 · Cited by 169
HIF-1-Independent Mechanisms Regulating Metabolic Adaptation in Hypoxic Cancer Cells
Lee, S.-H., Golinska, M., & Griffiths, J. R. (2021) · Cells · doi:10.3390/cells10092371 · Cited by 169
Glucose-dependent de Novo Lipogenesis in B Lymphocytes
Dufort, F. J., Gumina, M. R., Ta, N. L., Tao, Y., Heyse, S. A., Scott, D. A., Richardson, A. D., Seyfried, T. N., & Chiles, T. C. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.551051 · Cited by 160
Self-citation
Metabolic consequences of oncogenic IDH mutations
Parker, S. J., & Metallo, C. M. (2015) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2015.05.003 · Cited by 162
Targeting the Warburg effect via LDHA inhibition engages ATF4 signaling for cancer cell survival
Pathria, G., Scott, D. A., Feng, Y., Lee, J. S., Fujita, Y., Zhang, G., Sahu, A. D., Ruppin, E., Herlyn, M., Osterman, A. L., et al. (2018) · The EMBO Journal · doi:10.15252/embj.201899735 · Cited by 163
Self-citation
Metabolic interplay between glycolysis and mitochondrial oxidation: The reverse Warburg effect and its therapeutic implication
Lee, M. (2015) · World Journal of Biological Chemistry · doi:10.4331/wjbc.v6.i3.148 · Cited by 152
Metabolic Adaptations in Cancer Stem Cells
Yadav, U. P., Singh, T., Kumar, P., Sharma, P., Kaur, H., Sharma, S., Singh, S., Kumar, S., & Mehta, K. (2020) · Frontiers in Oncology · doi:10.3389/fonc.2020.01010 · Cited by 158
Inactivation of the HIF-1α/PDK3 Signaling Axis Drives Melanoma toward Mitochondrial Oxidative Metabolism and Potentiates the Therapeutic Activity of Pro-Oxidants
Kluza, J., Corazao-Rozas, P., Touil, Y. S., Jendoubi, M., Maire, C., Guerreschi, P., Jonneaux, A., Ballot, C., Balayssac, S., Valable, S., et al. (2012) · Cancer Research · doi:10.1158/0008-5472.can-12-0979 · Cited by 161
Targeting the Warburg effect in cancer cells through ENO1 knockdown rescues oxidative phosphorylation and induces growth arrest
Capello, M., Borgogno, S. F., Riganti, C., Chattaragada, M. S., Principe, M., Roux, C., Zhou, W., Petricoin, E. F., Cappello, P., & Novelli, F. (2015) · Oncotarget · doi:10.18632/oncotarget.6798 · Cited by 153
Proline dehydrogenase is essential for proline protection against hydrogen peroxide-induced cell death
Natarajan, S. K., Zhu, W., Liang, X., Zhang, L., Demers, A., Zimmerman, M. C., Simpson, M. A., & Becker, D. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.07.002 · Cited by 148
VDAC Regulation: A Mitochondrial Target to Stop Cell Proliferation
Fang, D., & Maldonado, E. N. (2018) · Advances in cancer research · doi:10.1016/bs.acr.2018.02.002 · Cited by 142
Metabolomic Analysis of Bone Morphogenetic Protein Receptor Type 2 Mutations in Human Pulmonary Endothelium Reveals Widespread Metabolic Reprogramming
Fessel, J. P., Hamid, R., Wittmann, B. M., Robinson, L. J., Blackwell, T., Tada, Y., Tanabe, N., Tatsumi, K., Hemnes, A. R., & West, J. D. (2012) · Pulmonary Circulation · doi:10.4103/2045-8932.97606 · Cited by 141
Alleviation of neuronal energy deficiency by mTOR inhibition as a treatment for mitochondria-related neurodegeneration
Zheng, X., Boyer, L. F., Jin, M., Kim, Y., Fan, W., Bardy, C., Berggren, W. T., Evans, R. M., Gage, F. H., & Hunter, T. R. (2016) · eLife · doi:10.7554/elife.13378 · Cited by 139
Molecular drivers of cellular metabolic reprogramming in melanoma
Abildgaard, C., & Guldberg, P. (2015) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2014.12.007 · Cited by 138
Global Profiling Strategies for Mapping Dysregulated Metabolic Pathways in Cancer
Benjamin, D. I., Cravatt, B. F., & Nomura, D. K. (2012) · Cell Metabolism · doi:10.1016/j.cmet.2012.09.013 · Cited by 131
Organochloride pesticides impaired mitochondrial function in hepatocytes and aggravated disorders of fatty acid metabolism
Liu, Q., Wang, Q., Xu, C. Y., Shao, W., Zhang, C., Liu, H., Jiang, Z., & Gu, A. (2017) · Scientific Reports · doi:10.1038/srep46339 · Cited by 127
On the Origin of ATP Synthesis in Cancer
Seyfried, T. N., Arismendi-Morillo, G., Mukherjee, P., & Chinopoulos, C. (2020) · iScience · doi:10.1016/j.isci.2020.101761 · Cited by 121
Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) Cycle
Gameiro, P. A., Laviolette, L. A., Kelleher, J. K., Iliopoulos, O., & Stephanopoulos, G. N. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.396796 · Cited by 126
The role of Hypoxia-Inducible Factor-1alpha and its signaling in melanoma
Malekan, M., Ebrahimzadeh, M. A., & Sheida, F. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2021.111873 · Cited by 123
Metabolic Plasticity of Melanoma Cells and Their Crosstalk With Tumor Microenvironment
Avagliano, A., Fiume, G., Pelagalli, A., Sanità, G., Ruocco, M. R., Montagnani, S., & Arcucci, A. (2020) · Frontiers in Oncology · doi:10.3389/fonc.2020.00722 · Cited by 128
Evaluation of integrin αvβ6 cystine knot PET tracers to detect cancer and idiopathic pulmonary fibrosis
Kimura, R. H., Wang, L., Shen, B., Huo, L., Tummers, W. S. F. J., Filipp, F. V., Guo, H. H., Haywood, T., Abou‐Elkacem, L., Baratto, L., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-11863-w · Cited by 120
Self-citation
Mitochondrial dysfunctions in cancer: Genetic defects and oncogenic signaling impinging on TCA cycle activity
Desideri, E., Vegliante, R., & Ciriolo, M. R. (2014) · Cancer Letters · doi:10.1016/j.canlet.2014.02.023 · Cited by 116
In vivo maps of extracellular pH in murine melanoma by CEST–MRI
Castelli, D. D., Ferrauto, G., Cutrìn, J. C., Terreno, E., & Aime, S. (2013) · Magnetic Resonance in Medicine · doi:10.1002/mrm.24664 · Cited by 113
Profiling metabolic networks to study cancer metabolism
Hiller, K., & Metallo, C. M. (2012) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2012.11.001 · Cited by 113
Glutamine‐fueled mitochondrial metabolism is decoupled from glycolysis in melanoma
Filipp, F. V., Ratnikov, B. I., Ingeniis, J. D., Smith, J. W., Osterman, A. L., & Scott, D. A. (2012) · Pigment Cell & Melanoma Research · doi:10.1111/pcmr.12000 · Cited by 115
Self-citation
Metabolism and epigenetics: a link cancer cells exploit
Carrer, A., & Wellen, K. E. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.11.012 · Cited by 110
Long non-coding RNA H19 promotes glucose metabolism and cell growth in malignant melanoma via miR-106a-5p/E2F3 axis
Luan, W., Zhou, Z., Ni, X., Xia, Y. F., Wang, J., Yan, Y., & Xu, B. (2018) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-018-2582-z · Cited by 110
Mitochondrial Respiration - An Important Therapeutic Target in Melanoma
de Moura, M. B., Vincent, G., Fayewicz, S. L., Bateman, N. W., Hood, B. L., Sun, M., Suhan, J. P., Duensing, S. M., Yin, Y., Sander, C., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0040690 · Cited by 105
Pyruvate Carboxylase Is Up-Regulated in Breast Cancer and Essential to Support Growth and Invasion of MDA-MB-231 Cells
Phannasil, P., Thuwajit, C., Warnnissorn, M., Wallace, J. C., MacDonald, M. J., & Jitrapakdee, S. (2015) · PLoS ONE · doi:10.1371/journal.pone.0129848 · Cited by 103
ROS production induced by BRAF inhibitor treatment rewires metabolic processes affecting cell growth of melanoma cells
Cesi, G., Walbrecq, G., Zimmer, A. D., Kreis, S., & Haan, C. (2017) · Molecular Cancer · doi:10.1186/s12943-017-0667-y · Cited by 108
Human Milk Oligosaccharides Promote the Growth of Staphylococci
Hunt, K. M., Preuss, J., Nissan, C., Davlin, C. A., Williams, J. E., Shafii, B., Richardson, A. D., McGuire, S., Bode, L., & McGuire, M. A. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.00477-12 · Cited by 104
Self-citation
Metabolic flexibility in melanoma: A potential therapeutic target
Ruocco, M. R., Avagliano, A., Granato, G., Vigliar, E., Masone, S., Montagnani, S., & Arcucci, A. (2019) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2019.07.016 · Cited by 106
Discovery of potential biomarkers in human melanoma cells with different metastatic potential by metabolic and lipidomic profiling
Kim, H.-Y., Lee, H., Kim, S., Jin, H., Bae, J., & Choi, H. (2017) · Scientific Reports · doi:10.1038/s41598-017-08433-9 · Cited by 104
Profiling and targeting of cellular bioenergetics: inhibition of pancreatic cancer cell proliferation
Cheng, G., Zielonka, J., McAllister, D. M., Tsai, S., Dwinell, M. B., & Kalyanaraman, B. (2014) · British Journal of Cancer · doi:10.1038/bjc.2014.272 · Cited by 90
Mitochondrial oxidative phosphorylation in cutaneous melanoma
Kumar, P. R., Moore, J. A., Bowles, K. M., Rushworth, S. A., & Moncrieff, M. D. (2020) · British Journal of Cancer · doi:10.1038/s41416-020-01159-y · Cited by 92
Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity
Hopperton, K. E., Duncan, R. E., Bazinet, R. P., & Archer, M. C. (2013) · Experimental Cell Research · doi:10.1016/j.yexcr.2013.10.016 · Cited by 91
L-Proline Induces a Mesenchymal-like Invasive Program in Embryonic Stem Cells by Remodeling H3K9 and H3K36 Methylation
Comes, S., Gagliardi, M., Laprano, N., Fico, A., Cimmino, A., Palamidessi, A., Cesare, D. D., Falco, S. D., Angelini, C., Scita, G., et al. (2013) · Stem Cell Reports · doi:10.1016/j.stemcr.2013.09.001 · Cited by 90
The metabolic microenvironment of melanomas: Prognostic value of MCT1 and MCT4
Pinheiro, C. M., Miranda‐Gonçalves, V., Longatto‐Filho, A., Vicente, A. L. S. A., Berardinelli, G. N., Scapulatempo‐Neto, C., Costa, R. F. A., Viana, C. R., Reis, R. M., Baltazar, F., et al. (2016) · Cell Cycle · doi:10.1080/15384101.2016.1175258 · Cited by 93
Activated lymphocytes as a metabolic model for carcinogenesis
Macintyre, A. N., & Rathmell, J. C. (2013) · Cancer & Metabolism · doi:10.1186/2049-3002-1-5 · Cited by 93
Press-pulse: a novel therapeutic strategy for the metabolic management of cancer
Seyfried, T. N., Yu, G. W., Maroon, J. C., & D’Agostino, D. P. (2017) · Nutrition & Metabolism · doi:10.1186/s12986-017-0178-2 · Cited by 86
Genetically-defined metabolic reprogramming in cancer
Mullen, A. R., & DeBerardinis, R. J. (2012) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2012.06.009 · Cited by 90
Roles of pyruvate carboxylase in human diseases: from diabetes to cancers and infection
Lao-On, U., Attwood, P. V., & Jitrapakdee, S. (2018) · Journal of Molecular Medicine · doi:10.1007/s00109-018-1622-0 · Cited by 85
Oncogene-Driven Metabolic Alterations in Cancer
Min, H., & Lee, H. (2017) · Biomolecules & Therapeutics · doi:10.4062/biomolther.2017.211 · Cited by 88
Disruption of Proline Synthesis in Melanoma Inhibits Protein Production Mediated by the GCN2 Pathway
Kardos, G. R., Wastyk, H. C., & Robertson, G. P. (2015) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-15-0048 · Cited by 84
Histone lactylation bridges metabolic reprogramming and epigenetic rewiring in driving carcinogenesis: Oncometabolite fuels oncogenic transcription
Zhang, Y., Song, H., Li, M., & Lu, P. (2024) · Clinical and Translational Medicine · doi:10.1002/ctm2.1614 · Cited by 84
Glutamate and asparagine cataplerosis underlie glutamine addiction in melanoma
Ratnikov, B. I., Aza‐Blanc, P., Ronai, Z. A., Smith, J. W., Osterman, A. L., & Scott, D. A. (2015) · Oncotarget · doi:10.18632/oncotarget.3132 · Cited by 80
Self-citation
Lipid metabolic Reprogramming: Role in Melanoma Progression and Therapeutic Perspectives
Pellerin, L., Carrié, L., Dufau, C., Nieto, L., Ségui, B., Levade, T., Riond, J., & Andrieu‐Abadie, N. (2020) · Cancers · doi:10.3390/cancers12113147 · Cited by 91
Metabolic and Bactericidal Effects of Targeted Suppression of NadD and NadE Enzymes in Mycobacteria
Rodionova, I. A., Schuster, B. M., Guinn, K. M., Sorci, L., Scott, D. A., Li, X., Kheterpal, I., Shoen, C. M., Cynamon, M. H., Locher, C. P., et al. (2014) · mBio · doi:10.1128/mbio.00747-13 · Cited by 82
Self-citation
Establishment of human iPSC-based models for the study and targeting of glioma initiating cells
Sancho-Martinez, I., Nivet, E., Xia, Y. F., Hishida, T., Aguirre, A., Ocampo, A., Ma, L., Morey, R. E., Krause, M. N., Zembrzycki, A., et al. (2016) · Nature Communications · doi:10.1038/ncomms10743 · Cited by 81
MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells
Mazar, J., Qi, F., Lee, B., Marchica, J., Govindarajan, S. S., Shelley, J. P., Li, J., Ray, A., & Perera, R. J. (2016) · Molecular and Cellular Biology · doi:10.1128/mcb.00762-15 · Cited by 80
Safety and outcome of treatment of metastatic melanoma using 3-bromopyruvate: a concise literature review and case study
Sayed, S. M. E., Mohamed, W. G., Seddik, M.-A. H., Ahmed, A.-S. A., Mahmoud, A. G., Amer, W. H., Nabo, M. M. H., Hamed, A., Ahmed, N. S., & Abd-Allah, A. A.-R. (2014) · 癌症：英文版 · doi:10.5732/cjc.013.10111 · Cited by 77
Exploiting tumor metabolism: challenges for clinical translation
Heiden, M. G. V. (2013) · Journal of Clinical Investigation · doi:10.1172/jci72391 · Cited by 79
MicroRNAs as regulators of mitochondrial function: Role in cancer suppression
Tomasetti, M., Neužil, J., & Dong, L. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.09.002 · Cited by 78
The Metabolic Origins of Mannose in Glycoproteins
Ichikawa, M., Scott, D. A., Losfeld, M., & Freeze, H. H. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.544064 · Cited by 76
Self-citation
VDAC–Tubulin, an Anti-Warburg Pro-Oxidant Switch
Maldonado, E. N. (2017) · Frontiers in Oncology · doi:10.3389/fonc.2017.00004 · Cited by 76
Influence of Tumor Microenvironment and Fibroblast Population Plasticity on Melanoma Growth, Therapy Resistance and Immunoescape
Romano, V., Belviso, I., Venuta, A., Ruocco, M. R., Masone, S., Aliotta, F., Fiume, G., Montagnani, S., Avagliano, A., & Arcucci, A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22105283 · Cited by 78
Inhibition of glioblastoma tumorspheres by combined treatment with 2-deoxyglucose and metformin
Kim, E. H., Lee, J.-H., Oh, Y., Koh, I., Shim, J., Park, J., Choi, J., Yun, M., Jeon, J. Y., Huh, Y., et al. (2016) · Neuro-Oncology · doi:10.1093/neuonc/now174 · Cited by 72
Inhibition of the pentose phosphate pathway by dichloroacetate unravels a missing link between aerobic glycolysis and cancer cell proliferation
Preter, G. D., Neveu, M.-A. C., Danhier, P., Brisson, L., Payen, V. L., Porporato, P. E., Jordan, B. F., Sonveaux, P., & Gallez, B. (2015) · Oncotarget · doi:10.18632/oncotarget.6272 · Cited by 69
Real‐Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In‐Cell NMR Spectroscopy
Wen, H., An, Y., Xu, W. J., Kang, K. W., & Park, S. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201410380 · Cited by 65
Isotope tracing in adult zebrafish reveals alanine cycling between melanoma and liver
Naser, F. J., Jackstadt, M. M., Fowle-Grider, R., Spalding, J. L., Cho, K., Stancliffe, E., Doonan, S. R., Kramer, E. T., Yao, L., Krasnick, B. A., et al. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.04.014 · Cited by 65
Chemotherapy Resistance: Role of Mitochondrial and Autophagic Components
Bahar, E., Han, S., Kim, J., & Yoon, H. (2022) · Cancers · doi:10.3390/cancers14061462 · Cited by 67
HuR is a post-transcriptional regulator of core metabolic enzymes in pancreatic cancer
Burkhart, R. A., Pineda, D. M., Chand, S. N., Romeo, C., Londin, E. R., Karoly, E. D., Cozzitorto, J. A., Rigoutsos, I., Yeo, C. J., Brody, J. R., et al. (2013) · RNA Biology · doi:10.4161/rna.25274 · Cited by 64
Time-resolved fluorescence lifetime for cutaneous melanoma detection
Pires, L., Nogueira, M. S., Pratavieira, S., Moriyama, L. T., & Kurachi, C. (2014) · Biomedical Optics Express · doi:10.1364/boe.5.003080 · Cited by 63

Authors: Scott, D. A., Richardson, A. D., Filipp, F. V., Knutzen, C. A., Chiang, G. G., Ronai, Z. A., Osterman, A. L., & Smith, J. W.
Affiliations: Sanford Burnham Prebys Medical Discovery Institute (US)
Corresponding author: Osterman, Andrei L.
ORCID: Scott, David A.
Richardson, Adam D.
Filipp, Fabian V.
Chiang, Gary G.
Ronai, Ze'ev A.
Osterman, Andrei L.
Smith, Jeffrey W.
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 2011-12
Volume: 286
Issue: 49
Pages: 42626-42634
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1074/jbc.m111.282046
PMC: PMC3234981
OpenAlex: W1976603231
Cited by: 328
References: 44
Citations by year: 2026: 7 · 2025: 21 · 2024: 16 · 2023: 15 · 2022: 21 · 2021: 32 · 2020: 30 · 2019: 21 · 2018: 22 · 2017: 25 · 2016: 20 · 2015: 33 · 2014: 20 · 2013: 20 · 2012: 25
FWCI: 7.07
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 3225 d)
Locations: Journal of Biological Chemistry (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Cancer Institute (CA-147978, R00CA154887, CA-154887, K99CA154887, R21CA147978, P01CA128814, CA-128814)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
Keywords: melanoma, Warburg effect, hypoxia, Pasteur effect, glycolysis, lactic acid production, TCA cycle, glutamine metabolism, reductive TCA cycle, fatty acid synthesis, melanocytes, isotopic labeling, flux balance analysis, metabolic therapy
MeSH terms: Hypoxia, Citric Acid Cycle, Fermentation, Glucose, Glutamine, Glycolysis, Humans, Ketoglutaric Acids, Gas Chromatography-Mass Spectrometry, Melanocytes, Melanoma, Models, Biological, Skin Neoplasms, Lactic Acid, Cell Line, Tumor

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Comparative Metabolic Flux Profiling of Melanoma Cell Lines

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks