CoChat
Sign Up
Cited by 155Fungal and yeast genetics researchPlant Stress Responses and ToleranceCalcium signaling and nucleotide metabolism

An osmotically induced cytosolic Ca2+ transient activates calcineurin signaling to mediate ion homeostasis and salt tolerance of Saccharomyces cerevisiae

Open access
Matsumoto, Tracie K., Ellsmore, Amanda J., Cessna, Stephen G., Low, Philip S., Pardo, Joseá M., Bressan, Ray A., Hasegawa, Paul M.·2002·Journal of Biological Chemistry
DOI 10.1074/jbc.m205037200
Open this paper in CoChat — free account

Abstract

Hyperosmotic stress caused by NaCl, LiCl, or sorbitol induces an immediate and short duration ( approximately 1 min) transient cytosolic Ca(2+) ([Ca(2+)](cyt)) increase (Ca(2+)-dependent aequorin luminescence) in Saccharomyces cerevisiae cells. The amplitude of the osmotically induced [Ca(2+)](cyt) transient was attenuated by the addition of chelating agents EGTA or BAPTA, cation channel pore blockers, competitive inhibitors of Ca(2+) transport, or mutations (cch1Delta or mid1Delta) that reduce Ca(2+) influx, indicating that Ca(ext)(2+) is a source for the transient. An osmotic pretreatment (30 min) administered by inoculating cells into media supplemented with either NaCl (0.4 or 0.5 m) or sorbitol (0.8 or 1.0 m) enhanced the subsequent growth of these cells in media containing 1 m NaCl or 2 m sorbitol. Inclusion of EGTA in the osmotic pretreatment media or the cch1Delta mutation reduced cellular capacity for NaCl but not hyperosmotic adaptation. The stress-adaptive effect of hyperosmotic pretreatment was mimicked by exposing cells briefly to 20 mm CaCl(2). Thus, NaCl- or sorbitol-induced hyperosmotic shock causes a [Ca(2+)](cyt) transient that is facilitated by Ca(2+) influx, which enhances ionic but not osmotic stress adaptation. NaCl-induced ENA1 expression was inhibited by EGTA, cch1Delta mutation, and FK506, indicating that the [Ca(2+)](cyt) transient activates calcineurin signaling to mediate ion homeostasis and salt tolerance.

Integrity signals

Registry

Crossref · 10.1074/jbc.m205037200

Cites

69 of 69 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Calcineurin is required in S. cerevisiae and S. pombe for adaptation to environmental stresses, cation homeostasis, morphogenesis, cell wall integrity, and mating.

    “Calcineurin has been well characterized in the model yeasts S. cerevisiae and Schizosaccharomyces pombe, where it is required for adaptation to a variety of environmental stresses, cation homeostasis, morphogenesis, cell wall integrity, and mating (Cyert et al., 1991; Matsumoto et al., 2002; Mendoza et al., 1996; Mendoza et al., 1994; Sugiura et al., 2002).”

    From Calcineurin as a multifunctional regulator: Unraveling novel functions in fungal stress responses, hyphal growth, drug resistance, and pathogenesis (2014) · cites this paper as Matsumoto et al., 2002

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Matsumoto, T. K., Ellsmore, A. J., Cessna, S. G., Low, P. S., Pardo, J. M., Bressan, R. A., & Hasegawa, P. M.
Affiliations
  • Purdue University West Lafayette (US)
  • Eastern Mennonite University (US)
Corresponding author
Hasegawa, Paul M.
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2002-09
Volume
277
Issue
36
Pages
33075-33080
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m205037200
OpenAlex
W2144489906
Cited by
155
References
69
Citations by year
2026: 1 · 2025: 2 · 2024: 10 · 2022: 5 · 2021: 8 · 2020: 5 · 2019: 7 · 2018: 3 · 2017: 6 · 2016: 10 · 2015: 9 · 2014: 11 · 2013: 6 · 2012: 2
FWCI
2.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
hyperosmotic stress, cytosolic calcium transients, calcineurin signaling, ion homeostasis, salt tolerance, ENA1, sodium chloride, sorbitol, calcium influx, MID1, osmoadaptation, Saccharomyces cerevisiae, FK506
MeSH terms
beta-Galactosidase, Calcium, Chelating Agents, Cytosol, Egtazic Acid, Ions, Models, Biological, Mutation, Osmosis, Saccharomyces cerevisiae, Salts, Sodium Chloride, Sorbitol, Time Factors, Signal Transduction, Calcineurin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review