Cited by 23Adipose Tissue and MetabolismMetabolism, Diabetes, and CancerExercise and Physiological Responses

BAIBA does not regulate UCP-3 expression in human skeletal muscle as a response to aerobic exercise

Morales, Flor E., Forsse, Jeffrey S., Andre, Thomas L., McKinley-Barnard, Sarah K., Hwang, Paul S., Anthony, Ian G., Tinsley, Grant M., Spillane, Mike +3 more·2017·Journal of the American College of Nutrition

Abstract

OBJECTIVE: β-Aminoisobutyric acid (BAIBA) has shown to modulate uncoupling protein (UCP)-1 expression, which is mainly expressed in white adipose tissue; however, no studies to date have analyzed its potential effect on the main uncoupling protein of skeletal muscle, UCP-3. The main goal of this study was to assess the potential effect of acute aerobic exercise on serum BAIBA and skeletal muscle UCP-3. The secondary goal was to assess the potential involvement of the transcription factors proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and peroxisome proliferator-activated receptor alpha (PPARα), as well as free fatty acids (FFAs) in UCP-3 expression. A tertiary goal of the study was to evaluate the potential effect of consuming a preexercise meal on the outcome of the first 2 objectives. METHODS: ) after 2 experimental conditions: (1) consumption of a multi-macronutrient shake and (2) a fasting period of 8 hours. Blood samples were taken at baseline, preexercise, postexercise, 1 hour, and 4 hours postexercise, and muscle biopsies were taken at the last 4 time points. UCP-3 protein concentration and expression, as well as the mRNA expression of PGC-1α and PPARα, were measured in muscle, and BAIBA, glucose, and FFA were measured in serum. RESULTS: Aerobic exercise failed to induce a significant effect on serum BAIBA, PGC-1α, and PPARα regardless on the feeding condition. Despite the lack of effect of exercise on the previous variables, UCP-3 expression and protein concentration significantly increased in the shake condition. CONCLUSION: The expression of human skeletal muscle UCP-3 as a result of exercise might be controlled by factors other than BAIBA.

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Crossref · 10.1080/07315724.2016.1256240

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Authors
Morales, F. E., Forsse, J. S., Andre, T. L., McKinley-Barnard, S. K., Hwang, P. S., Anthony, I. G., Tinsley, G. M., Spillane, M., Grandjean, P. W., Ramirez, A., & Willoughby, D. S.
Affiliations
  • Baylor University (US)
  • Department of Health, Human Performance and Recreation, Baylor University, Waco, Texas
  • University of South Alabama (US)
  • Texas Tech University (US)
  • Tecnológico de Monterrey (MX)
Corresponding author
Willoughby, Darryn S.
Venue
Journal of the American College of Nutrition
Published
2017-03-20
Volume
36
Issue
3
Pages
200-209
ISSN
  • 0731-5724 (ISSN-L)
  • 1541-1087
OpenAlex
W2598580195
Cited by
23
References
43
Citations by year
2026: 1 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 4
FWCI
1.62
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of the American College of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Funders
Baylor University
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
beta-aminoisobutyric acid, UCP3, skeletal muscle, aerobic exercise, PGC-1α, PPARα, free fatty acids, muscle biopsy
MeSH terms
Uncoupling Protein 3, Adolescent, Adult, Aminoisobutyric Acids, Gene Expression Regulation, Humans, Male, Transcription Factors, Exercise, Muscle, Skeletal, Cross-Over Studies, Young Adult

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