High Peroxidase Activity In Cell Cultures of Artemisia Annua With Minute Artemisinin Contents
Abstract
In callus and cell suspension cultures of Artemisia annua minute amounts of artemisinin were found. In these cultures, a high peroxidase activity was measured, intracellularly and, especially, in the medium. In vitro, artemisinin rapidly decomposed when incubated with a cell homogenate of A. annua or with spent culture medium, as well as in a solution of commercially obtained horseradish peroxidase (EC 1.11.1.7). In contrast, the peroxidase activity in the intact plant, containing considerable amounts of artemisinin, was low. We suggest that the high peroxidase activity in A. annua cell cultures contributes to their very low artemisinin contents.
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Correlations have been observed between peroxidase activity and artemisinin levels in hairy roots.
“Correlations have been observed between peroxidase activity and artemisinin levels in hairy roots [354]”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [354]
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The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao)
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- Authors
- Woerdenbag, H. J., Pras, N., Uden, W. V., Boer, A. D., Batterman, S., Visser, J. F., & Malingré, T. M.
- Affiliations
- University of Groningen (NL)
- Venue
- Natural product letters
- Publisher
- Informa UK Limited
- Published
- 1992-09
- Volume
- 1
- Issue
- 2
- Pages
- 121-128
- ISSN
- 1026-8049 (ISSN-L)
- 1057-5634
- OpenAlex
- W2068187368
- Cited by
- 24
- References
- 29
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2014: 1 · 2012: 1
- FWCI
- 0.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Natural product letters (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Keywords
- Artemisia annua, artemisinin, peroxidase activity, cell suspension culture, callus culture, horseradish peroxidase, in vitro biosynthesis
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