Antifungal effect of organic acids from lactic acid bacteria on Penicillium nordicum
Open accessAbstract
The control of fungal contamination is particularly important to avoid both spoilage of food and feed products and the occurrence of toxic compounds, known as mycotoxins. Some lactic acid bacteria (LAB) strains have shown the capacity to inhibit fungal growth and the production of mycotoxins. In this work, cell-free supernatants (CFS) of Lactobacillus plantarum UM55 and Lactobacillus buchneri UTAD104 were tested against Penicillium nordicum radial growth and OTA production. When CFS of these strains were used, the radial growth of the fungus was inhibited by less than 20%, but the production of OTA was reduced by approx. 60%. These antifungal effects resulted from organic acids produced by LAB. The CFS of L. plantarum UM55 contained lactic acid, phenyllactic acid (PLA), hydroxyphenyllactic acid (OH-PLA) and indole lactic acid (ILA), while L. buchneri UTAD104 CFS contained acetic acid, lactic acid and PLA. These organic acids were further tested individually for their inhibitory capacity. Calculation of the inhibitory concentrations (ICs) showed that acetic acid, ILA and PLA were the most effective in inhibiting P. nordicum growth and OTA production. When the inhibitory activity of LAB cells incorporated into the culture medium was tested, L. buchneri UTAD104 inhibited the production of OTA entirely in all conditions tested, but fungal growth was only inhibited completely by the highest concentrations of cells. Acetic acid production was primarily responsible for this effect.…
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Guimarães and Venancio (2018) found that 8 mg/mL PLA completely inhibited Penicillium nordicum.
“Similarly, Guimarães and Venancio (2018) reported complete inhibition of Penicillium nordicum at 8 mg/mL of PLA, while Cortés-Zavaleta et al. (2014) determined the minimum inhibitory concentration (MIC) of PLA against C. gloeosporioides to be 0.5 mg/mL.”
From Antifungal Potential of Chitosan and Phenyllactic Acid against Colletotrichum gloeosporioides, the Anthracnose Pathogen in Avocados (2025) · cites this paper as Guimarães and Venancio 2018
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“As mentioned before, acetic acid is a more potent antifungal metabolite than lactic acid (Ranjit and Kung 2000).”
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Antifungal 3-Hydroxy Fatty Acids from <i>Lactobacillus plantarum</i> MiLAB14
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BackgroundMethodMetabolite profiles of lactic acid bacteria in grass silage
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Anti-aflatoxigenic effect of organic acids produced by Lactobacillus plantarum
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Lactic acid bacteria as antifungal and anti-mycotoxigenic agents: A comprehensive review
Sadiq, F. A., Yan, B., Tian, F., Zhao, J., Zhang, H., & Chen, W. (2019) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12481 · Cited by 313 · see citing works
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- Authors
- Guimarães, A., Venancio, A., & Abrunhosa, L.
- Affiliations
- University of Minho (PT)
- CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal
- Venue
- Food Additives & Contaminants: Part A
- Publisher
- Informa UK Limited
- Published
- 2018-08-06
- Volume
- 35
- Issue
- 9
- Pages
- 1803-1818
- ISSN
- 1944-0049 (ISSN-L)
- 1944-0057
- OpenAlex
- W2767922233
- Cited by
- 122
- References
- 9
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 19 · 2023: 23 · 2022: 22 · 2021: 14 · 2020: 16 · 2019: 4
- FWCI
- 7.51
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- other-oa
- Locations
- Food Additives & Contaminants Part A (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Funders
- Fundação para a Ciência e a Tecnologia (UMINHO/BPD/51/2015, UID/BIO/04469/2013)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- lactic acid bacteria, Penicillium nordicum, organic acids, antifungal activity, mycotoxin production, ochratoxin A, Lactobacillus plantarum, Lactobacillus buchneri, phenyllactic acid, acetic acid, lactic acid, cell-free supernatant, fungal growth inhibition, dry-cured meat, cheese
- MeSH terms
- Antifungal Agents, Microbial Sensitivity Tests, Penicillium, Acetic Acid, Lactic Acid, Lactobacillales
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