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Cited by 422Chemokine receptors and signalingImmune cells in cancerImmune Response and Inflammation

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CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation

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Auffray, Cedric, Fogg, Darin K., Narni-Mancinelli, Emilie, Senechal, Brigitte, Trouillet, Celine, Saederup, Noah, Leemput, Julia, Bigot, Karine, Campisi, Laura, Abitbol, Marc, Molina, Thierry, Charo, Israel, Hume, David A., Cumano, Ana, Lauvau, Gregoire, Geissmann, Frederic·2009·Journal of Experimental Medicine
DOI 10.1084/jem.20081385PMC PMC2699130
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Abstract

CX3CR1 expression is associated with the commitment of CSF-1R+ myeloid precursors to the macrophage/dendritic cell (DC) lineage. However, the relationship of the CSF-1R+ CX3CR1+ macrophage/DC precursor (MDP) with other DC precursors and the role of CX3CR1 in macrophage and DC development remain unclear. We show that MDPs give rise to conventional DCs (cDCs), plasmacytoid DCs (PDCs), and monocytes, including Gr1+ inflammatory monocytes that differentiate into TipDCs during infection. CX3CR1 deficiency selectively impairs the recruitment of blood Gr1+ monocytes in the spleen after transfer and during acute Listeria monocytogenes infection but does not affect the development of monocytes, cDCs, and PDCs.

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Figure 1. MDP and CDP share a similar phenotype. BM cells from Cx3cr1gfp/+ reporter mice were labeled with antibodies against lineage markers (CD11c, CD11b, NK1.1, CD3, Ter119, CD19, and Gr1), ckit (CD117), FLT3 (CD135), and CSF-1-R (CD115) and analyzed by FACS. CDP and MDP are overlapping populations; lineage− CD117low CD135+ CD115+ cells (CDP) express CX3CR1 (gfp), whereas CD117int CX3CR1 CD115+ cells (MDP) express CD135. Results are representative of >10 experiments.
Figure 1. MDP and CDP share a similar phenotype. BM cells from Cx3cr1gfp/+ reporter mice were labeled with antibodies against lineage markers (CD11c, CD11b, NK1.1, CD3, Ter119, CD19, and Gr1), ckit (CD117), FLT3 (CD135), and CSF-1-R (CD115) and analyzed by FACS. CDP and MDP are overlapping populations; lineage− CD117low CD135+ CD115+ cells (CDP) express CX3CR1 (gfp), whereas CD117int CX3CR1 CD115+ cells (MDP) express CD135. Results are representative of >10 experiments.CC BY-NC-SA · PubMed Central
Figure 2. Differentiation potential of MDP in vivo. (a–c) MDPs from Cd45.1/2 or Cd45.2 Cx3cr1gfp/+ reporter mice were purified as described in Fig. S2 (available at http://www.jem.org/cgi/content/full/jem.20081385/DC1) and were adoptively transferred into irradiated (900 rad) C57BL/6 Cd45.2 congenic recipients. Spleens of recipient mice were analyzed at day 7 after transfer by flow cytometry, using lineage marker (NK1.1 CD3 CD19), CD11b, CD11c, and CD8-α antibodies. R1 corresponds to PDCs (Lin− CD11cint CD11b− CX3CR1+ cells), R2 and R3 correspond to cDCs (lin− CD11chigh CD11b− CX3CR1− cells and lin− CD11chigh CD11b+ CX3CR1+ cells), and R4 corresponds to monocytes (lin− CD11b+ CD11c− CX3CR1+ cells). The experiment was repeated five times with two to three mice per group and with similar results. (b) Donor-derived Lin− CD11cint CD11b− CX3CR1+ cells express PDCA1. (c) Role of anti-CD115 Ab. The number of donor-derived cells per spleen are represented after adoptive transfer of MDP, purified with or without CD115 antibody (n = at least 3 mice per group from two experiments). Error bars show SD. (d) The flow diagram represent the Lin− CD117int CD115+ CD135+ CX3CR1+ MDPs that give rise to monocytes, cDCs, and PDCs and their putative relationship with other myeloid precursors.
Figure 2. Differentiation potential of MDP in vivo. (a–c) MDPs from Cd45.1/2 or Cd45.2 Cx3cr1gfp/+ reporter mice were purified as described in Fig. S2 (available at http://www.jem.org/cgi/content/full/jem.20081385/DC1) and were adoptively transferred into irradiated (900 rad) C57BL/6 Cd45.2 congenic recipients. Spleens of recipient mice were analyzed at day 7 after transfer by flow cytometry, using lineage marker (NK1.1 CD3 CD19), CD11b, CD11c, and CD8-α antibodies. R1 corresponds to PDCs (Lin− CD11cint CD11b− CX3CR1+ cells), R2 and R3 correspond to cDCs (lin− CD11chigh CD11b− CX3CR1− cells and lin− CD11chigh CD11b+ CX3CR1+ cells), and R4 corresponds to monocytes (lin− CD11b+ CD11c− CX3CR1+ cells). The experiment was repeated five times with two to three mice per group and with similar results. (b) Donor-derived Lin− CD11cint CD11b− CX3CR1+ cells express PDCA1. (c) Role of anti-CD115 Ab. The number of donor-derived cells per spleen are represented after adoptive transfer of MDP, purified with or without CD115 antibody (n = at least 3 mice per group from two experiments). Error bars show SD. (d) The flow diagram represent the Lin− CD117int CD115+ CD135+ CX3CR1+ MDPs that give rise to monocytes, cDCs, and PDCs and their putative relationship with other myeloid precursors.CC BY-NC-SA · PubMed Central
Figure 3. CX3CR1 is important for the development of CD11b+ CD11c− monocytes. (a) MDP numbers in BM from CX3CR1+/− (black) and CX3CR1−/− (white) mice. Data are the mean ± SD of five mice per group. (b–d) Competitive adoptive transfer of MDP into irradiated host (900 rad). Equal numbers (104) of MDPs from Cd45.1/2 mice (blue) and Cd45.2/2 mice (red) were mixed and adoptively transferred into a Cd45.1 congenic recipient. Spleens of recipient mice were analyzed at day 7 after transfer by flow cytometry, using lineage marker (NK1.1 CD3 CD19), CD11b, CD11c, and CD8-α antibodies. (b and c) When both Cd45.1/2 and Cd45.2/2 donor mice were of the Cx3cr1+/− genotype, both donors contributed equally to NK1.1− CD3− CD19− CD11chigh CD11b− (R1, cDC) and NK1.1− CD3− CD19− CD11chigh CD11b+ (R2, cDC), NK1.1− CD3− CD19− CD11cint CD11b− (R3, PDC), and NK1.1− CD3− CD19− CD11b+ CD11c− splenocytes (R4). (b and d) In contrast, when Cd45.1/2 donor mice were of the Cx3cr1+/− genotype and Cd45.2/2 donor mice were of the Cx3cr1−/− genotype (CX3CR1 deficient), both donors contributed equally to CD11c+ cDCs in R1 and R2 but Cd45.1/2 Cx3cr1+/− MDPs were 10× more efficient than Cd45.2/2 Cx3cr1-deficient MDPs in generating CD11b+ CD11c− splenocytes in R3. Results in b are from one representative experiment out of three, with two to three mice per experimental group, and bar graphs in c and d represent the mean and SD from three independent experiments. The asterisk indicates a significative difference between groups (P < 0.05 using the Wilcoxon test).
Figure 3. CX3CR1 is important for the development of CD11b+ CD11c− monocytes. (a) MDP numbers in BM from CX3CR1+/− (black) and CX3CR1−/− (white) mice. Data are the mean ± SD of five mice per group. (b–d) Competitive adoptive transfer of MDP into irradiated host (900 rad). Equal numbers (104) of MDPs from Cd45.1/2 mice (blue) and Cd45.2/2 mice (red) were mixed and adoptively transferred into a Cd45.1 congenic recipient. Spleens of recipient mice were analyzed at day 7 after transfer by flow cytometry, using lineage marker (NK1.1 CD3 CD19), CD11b, CD11c, and CD8-α antibodies. (b and c) When both Cd45.1/2 and Cd45.2/2 donor mice were of the Cx3cr1+/− genotype, both donors contributed equally to NK1.1− CD3− CD19− CD11chigh CD11b− (R1, cDC) and NK1.1− CD3− CD19− CD11chigh CD11b+ (R2, cDC), NK1.1− CD3− CD19− CD11cint CD11b− (R3, PDC), and NK1.1− CD3− CD19− CD11b+ CD11c− splenocytes (R4). (b and d) In contrast, when Cd45.1/2 donor mice were of the Cx3cr1+/− genotype and Cd45.2/2 donor mice were of the Cx3cr1−/− genotype (CX3CR1 deficient), both donors contributed equally to CD11c+ cDCs in R1 and R2 but Cd45.1/2 Cx3cr1+/− MDPs were 10× more efficient than Cd45.2/2 Cx3cr1-deficient MDPs in generating CD11b+ CD11c− splenocytes in R3. Results in b are from one representative experiment out of three, with two to three mice per experimental group, and bar graphs in c and d represent the mean and SD from three independent experiments. The asterisk indicates a significative difference between groups (P < 0.05 using the Wilcoxon test).CC BY-NC-SA · PubMed Central
Figure 4. CX3CR1 is important for the recruitment of CD11b+ CD11c− monocytes to the spleen during infection. (a and b) Mouse fractalkine/CX3CL1 transcripts are detected in spleen tissue sections from WT mice around B cells follicles in the marginal zone/T cell area. Bars: (a) 500 µm; (b) 200 µm. (c) Short-term adoptive transfer of BM monocytes into irradiated WT recipient mice. Equal numbers (2 × 105) of BM monocytes from Cx3cr1+/− Cd45.1/2 mice (blue) and Cx3cr1−/− Cd45.2/2 mice (red) were mixed and adoptively transferred into a Cd45.1 congenic recipient. CX3CR1-expressing CD45.1/2 Gr1+ monocytes are recruited in the spleen of irradiated host 10× more efficiently than CX3CR1-deficient monocytes. The experiment was performed three times with two to three mice per group with similar results. (d–f) Monocyte recruitment in the spleen of infected mice. (d) BALB/c Cx3cr1+/− and Cx3cr1−/− mice (seven per group) were infected i.v. with live Lm (3 × 105), and monocytes (CX3CR1− gfp+ CD11b+ Ly-6C+ Ly-6G−, CD19−, CD3−, and NK1.1−) were enumerated after 24 h in the blood and spleen of infected and control uninfected mice. (e) C57BL/6 Cx3cr1+/− and Cx3cr1−/− mice (seven per group) were injected with 7 × 105 Lm, and monocytes were enumerated after 24 h in the blood and spleen of infected mice. (f) C57BL/6 Cx3cr1+/− and Cx3cr1−/− mice (seven per group) were injected with 7 × 103 Lm, and monocytes were enumerated after 48 h in the spleen of infected mice. Lm growth in the spleen of infected mice is shown. The asterisks indicate a significative difference between groups (P < 0.05). (g) Absence of proliferation of monocytes in the spleen of Lm-infected mice. BALB/c mice (n = 6 per group) were infected with 3 × 103 bacteria, spleen and leg bones were harvested 16 and 48 h later, and cells were processed as indicated in Fig. S5 (available at http://www.jem.org/cgi/content/full/jem.20081385/DC1). Data indicate the percentage of BM precursors and spleen monocytes in G0/G1 and in G2+S as analyzed by flow cytometry after DNA labeling with PI. The experiment was performed three times with similar results. (h) BALB/c Cx3cr1+/+ and Cx3cr1−/− mice were infected i.v. with live Lm (3 × 103). Data show the number of bacteria (mean ± SE) in the spleen 24 h after infection. (i) C57BL/6 Cx3cr1+/+ and Cx3cr1−/− mice were injected with 104 Lm. Data show the number of bacteria (mean ± SE) in the spleen 24 h after infection. Circles represent individual mice.
Figure 4. CX3CR1 is important for the recruitment of CD11b+ CD11c− monocytes to the spleen during infection. (a and b) Mouse fractalkine/CX3CL1 transcripts are detected in spleen tissue sections from WT mice around B cells follicles in the marginal zone/T cell area. Bars: (a) 500 µm; (b) 200 µm. (c) Short-term adoptive transfer of BM monocytes into irradiated WT recipient mice. Equal numbers (2 × 105) of BM monocytes from Cx3cr1+/− Cd45.1/2 mice (blue) and Cx3cr1−/− Cd45.2/2 mice (red) were mixed and adoptively transferred into a Cd45.1 congenic recipient. CX3CR1-expressing CD45.1/2 Gr1+ monocytes are recruited in the spleen of irradiated host 10× more efficiently than CX3CR1-deficient monocytes. The experiment was performed three times with two to three mice per group with similar results. (d–f) Monocyte recruitment in the spleen of infected mice. (d) BALB/c Cx3cr1+/− and Cx3cr1−/− mice (seven per group) were infected i.v. with live Lm (3 × 105), and monocytes (CX3CR1− gfp+ CD11b+ Ly-6C+ Ly-6G−, CD19−, CD3−, and NK1.1−) were enumerated after 24 h in the blood and spleen of infected and control uninfected mice. (e) C57BL/6 Cx3cr1+/− and Cx3cr1−/− mice (seven per group) were injected with 7 × 105 Lm, and monocytes were enumerated after 24 h in the blood and spleen of infected mice. (f) C57BL/6 Cx3cr1+/− and Cx3cr1−/− mice (seven per group) were injected with 7 × 103 Lm, and monocytes were enumerated after 48 h in the spleen of infected mice. Lm growth in the spleen of infected mice is shown. The asterisks indicate a significative difference between groups (P < 0.05). (g) Absence of proliferation of monocytes in the spleen of Lm-infected mice. BALB/c mice (n = 6 per group) were infected with 3 × 103 bacteria, spleen and leg bones were harvested 16 and 48 h later, and cells were processed as indicated in Fig. S5 (available at http://www.jem.org/cgi/content/full/jem.20081385/DC1). Data indicate the percentage of BM precursors and spleen monocytes in G0/G1 and in G2+S as analyzed by flow cytometry after DNA labeling with PI. The experiment was performed three times with similar results. (h) BALB/c Cx3cr1+/+ and Cx3cr1−/− mice were infected i.v. with live Lm (3 × 103). Data show the number of bacteria (mean ± SE) in the spleen 24 h after infection. (i) C57BL/6 Cx3cr1+/+ and Cx3cr1−/− mice were injected with 104 Lm. Data show the number of bacteria (mean ± SE) in the spleen 24 h after infection. Circles represent individual mice.CC BY-NC-SA · PubMed Central
Figure 5. Functions of spleen CD115+ Gr1+/TipDCs. (a) CD11b+ spleen cells from Cx3cr1gfp/+ reporter mice negative for lineage marker (NK1.1, CD3, B220, CD19, and CD11c) were analyzed for Ly6G, Ly6C, F4/80, CD115, and Cx3CR1 (gfp) expression by multicolor flow cytometry. (b) TNF production by lineage− CD11clow CD11b+ cells 24 h after i.v. infection with Lm. Gated cells (circled) are displayed in red. Data show representative FACS profiles from three experiments. (c and d) Production of TNF-α, iNOS, and ROI by lineage− CD11clow CD11b+ Gr1+/Ly6C+ spleen cells from C57BL/6 mice 24 (c) and 48 h (d) after i.v. infection with 7 × 105 bacteria and 7 × 103 bacteria, respectively. The experiment was repeated five times with similar results. Error bars show SD. (e) iNOS+ cells in the spleen of BALB/c mice 24 h after infection with 105 WT Lm (red) express CD11b and Ly6C and are found in the perifollicular area and T cell area. B cell follicles are labeled with anti-B220 antibodies (green) and T cells are labeled with anti-CD3 antibodies (blue). Bar, 100 µm. The experiment was repeated five times with similar results.
Figure 5. Functions of spleen CD115+ Gr1+/TipDCs. (a) CD11b+ spleen cells from Cx3cr1gfp/+ reporter mice negative for lineage marker (NK1.1, CD3, B220, CD19, and CD11c) were analyzed for Ly6G, Ly6C, F4/80, CD115, and Cx3CR1 (gfp) expression by multicolor flow cytometry. (b) TNF production by lineage− CD11clow CD11b+ cells 24 h after i.v. infection with Lm. Gated cells (circled) are displayed in red. Data show representative FACS profiles from three experiments. (c and d) Production of TNF-α, iNOS, and ROI by lineage− CD11clow CD11b+ Gr1+/Ly6C+ spleen cells from C57BL/6 mice 24 (c) and 48 h (d) after i.v. infection with 7 × 105 bacteria and 7 × 103 bacteria, respectively. The experiment was repeated five times with similar results. Error bars show SD. (e) iNOS+ cells in the spleen of BALB/c mice 24 h after infection with 105 WT Lm (red) express CD11b and Ly6C and are found in the perifollicular area and T cell area. B cell follicles are labeled with anti-B220 antibodies (green) and T cells are labeled with anti-CD3 antibodies (blue). Bar, 100 µm. The experiment was repeated five times with similar results.CC BY-NC-SA · PubMed Central

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Authors
Auffray, C., Fogg, D. K., Narni-Mancinelli, E., Senechal, B., Trouillet, C., Saederup, N., Leemput, J., Bigot, K., Campisi, L., Abitbol, M., Molina, T., Charo, I., Hume, D. A., Cumano, A., Lauvau, G., & Geissmann, F.
Affiliations
  • Laboratory of Biology of the Mononuclear Phagocyte System, Institut National de la Santé et de la Recherche Médicale (INSERM) U838, Université Paris-Descartes, 75015 Paris, France 1
  • Inserm (FR)
  • INSERM U924, Université de Nice-Sophia Antipolis, 06560 Valbonne, France 2
  • University of California, San Francisco (US)
  • Université Paris Descartes (FR)
  • Roslin Institute (GB)
ORCID
Venue
Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
2009-03-09
Volume
206
Issue
3
Pages
595-606
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.20081385
PMC
PMC2699130
OpenAlex
W2142750845
Cited by
422
References
50
Citations by year
2026: 4 · 2025: 7 · 2024: 14 · 2023: 17 · 2022: 16 · 2021: 21 · 2020: 17 · 2019: 26 · 2018: 21 · 2017: 28 · 2016: 32 · 2015: 24 · 2014: 33 · 2013: 43 · 2012: 36
FWCI
19.67
Type
journal article
Language
en
Open access
Locations
  • The Journal of Experimental Medicine (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, other-oa)
  • HAL (submittedVersion, other-oa)
  • Research Portal (King's College London) (submittedVersion)
Topics
  • Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
CX3CR1, CSF1R, myeloid dendritic cells, plasmacytoid dendritic cells, monocytes, Listeria monocytogenes infection, monocyte recruitment, spleen, inflammation, myeloid development
MeSH terms
CX3C Chemokine Receptor 1, Animals, Bone Marrow Cells, Cell Differentiation, Cell Movement, Cell Survival, Dendritic Cells, Inflammation, Listeriosis, Listeria monocytogenes, Macrophages, Monocytes, Phenotype, Spleen, Stem Cells, Tumor Necrosis Factor-alpha, Receptor, Macrophage Colony-Stimulating Factor, Reactive Oxygen Species, Receptors, Chemokine, Cell Proliferation, Mice, fms-Like Tyrosine Kinase 3, Nitric Oxide Synthase Type II

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