Cited by 1,152Chemokine receptors and signalingImmune cells in cancerInflammation biomarkers and pathways

Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites

Open access
Tsou, Chia-Lin, Peters, Wendy, Si, Yue, Slaymaker, Sarah, Aslanian, Ara M., Weisberg, Stuart P., Mack, Matthias, Charo, Israel F.·2007·Journal of Clinical Investigation

Abstract

Monocyte recruitment to sites of inflammation is regulated by members of the chemokine family of chemotactic cytokines. However, the mechanisms that govern the migration of monocytes from bone marrow to blood and from blood to inflamed tissues are not well understood. Here we report that CC chemokine receptor 2 (CCR2) is highly expressed on a subpopulation of blood monocytes whose numbers are markedly decreased in CCR2(-/-) mice. In bone marrow, however, CCR2(-/-) mice had an increased number of monocytes, suggesting that CCR2 is critical for monocyte egress. Intravenous infusion of ex vivo-labeled WT or CCR2(-/-) bone marrow into WT recipient mice demonstrated that CCR2 is necessary for efficient monocyte recruitment from the blood to inflamed tissue. Analysis of mice lacking monocyte chemoattractant protein-1 (MCP-1), MCP-3, MCP-5, or MCP-2 plus MCP-5 revealed that MCP-3 and MCP-1 are the CCR2 agonists most critical for the maintenance of normal blood monocyte counts. These findings provide evidence that CCR2 and MCP-3/MCP-1 are critical for monocyte mobilization and suggest new roles for monocyte chemoattractants in leukocyte homeostasis.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1172/jci29919

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The CCL2-CCR2 and CX3CL1-CX3CR1 chemotactic axes are two ligand-receptor signaling pathways that recruit monocytes to sites of inflammation, including the brain.

    “the CCL2-CCR2 and CX3CL1-CX3CR1 chemotactic axis are two ligand-receptor signaling pathways that recruit monocytes to sites of inflammation (Auffray et al, 2007;Tsou et al, 2007), including the brain (D' Mello et al, 2009).”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Tsou, C.-L., Peters, W., Si, Y., Slaymaker, S., Aslanian, A. M., Weisberg, S. P., Mack, M., & Charo, I. F.
Affiliations
  • University of California, San Francisco (US)
  • Gladstone Institutes (US)
  • Columbia University Irving Medical Center (US)
  • Columbia University (US)
Corresponding author
Charo, Israel F.
Venue
Journal of Clinical Investigation
Published
2007-04-02
Volume
117
Issue
4
Pages
902-909
ISSN
0021-9738 (ISSN-L)
OpenAlex
W2010472544
Cited by
1,152
References
34
Citations by year
2026: 53 · 2025: 55 · 2024: 75 · 2023: 54 · 2022: 70 · 2021: 64 · 2020: 61 · 2019: 46 · 2018: 57 · 2017: 68 · 2016: 80 · 2015: 59 · 2014: 66 · 2013: 69 · 2012: 55
FWCI
26.15
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Investigation (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (R01HL063894, R01HL052773, 5R01 HL52773-12, R01 HL63894-07)
Topics
  • Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
  • Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
Keywords
CCR2, MCP-3, MCP-1, monocyte mobilization, monocyte recruitment, chemokines, bone marrow, inflammation, CCR2-deficient mice
MeSH terms
Animals, Blood Cell Count, Bone Marrow Cells, Humans, Inflammation, Monocytes, Polymorphism, Genetic, Bone Marrow Transplantation, Mice, Knockout, Chemokines, Monocyte Chemoattractant Proteins, Adoptive Transfer, Receptors, Chemokine, Mice, Receptors, CCR2, Chemokine CCL7

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review