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Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels
Open accessAbstract
Fabrication of three dimensional (3D) organoids with controlled microarchitectures has been shown to enhance tissue functionality. Bioprinting can be used to precisely position cells and cell-laden materials to generate controlled tissue architecture. Therefore, it represents an exciting alternative for organ fabrication. Despite the rapid progress in the field, the development of printing processes that can be used to fabricate macroscale tissue constructs from ECM-derived hydrogels has remained a challenge. Here we report a strategy for bioprinting of photolabile cell-laden methacrylated gelatin (GelMA) hydrogels. We bioprinted cell-laden GelMA at concentrations ranging from 7 to 15% with varying cell densities and found a direct correlation between printability and the hydrogel mechanical properties. Furthermore, encapsulated HepG2 cells preserved cell viability for at least eight days following the bioprinting process. In summary, this work presents a strategy for direct-write bioprinting of a cell-laden photolabile ECM-derived hydrogel, which may find widespread application for tissue engineering, organ printing and the development of 3D drug discovery platforms.
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“By adapting this method, we avoided potentially harsh printing conditions including, low temperature for a prolonged printing period [51] and undesirable shear stress along the length of the nozzle [30].”
From A liver-on-a-chip platform with bioprinted hepatic spheroids (2016) · cites this paper as [30]
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Metadata
- Authors
- Bertassoni, L. E., Cardoso, J. C., Manoharan, V., Cristino, A. L., Bhise, N. S., Araujo, W. A., Zorlutuna, P., Vrana, N. E., Ghaemmaghami, A. M., Dokmeci, M. R., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- University of Nottingham (GB)
- Corresponding author
- Dokmeci, Mehmet R
- Khademhosseini, Ali
- ORCID
- Venue
- Biofabrication
- Publisher
- IOP Publishing
- Published
- 2014-04-03
- Volume
- 6
- Issue
- 2
- Pages
- 024105
- ISSN
- 1758-5082 (ISSN-L)
- 1758-5090
- PMC
- PMC4040163
- OpenAlex
- W2100081159
- Cited by
- 642
- References
- 37
- Citations by year
- 2026: 17 · 2025: 36 · 2024: 34 · 2023: 44 · 2022: 55 · 2021: 69 · 2020: 72 · 2019: 80 · 2018: 74 · 2017: 68 · 2016: 56 · 2015: 32 · 2014: 4
- FWCI
- 27.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biofabrication (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
- Repository@Nottingham (University of Nottingham) (submittedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- National Institutes of Health (EB02597)
- Defense Threat Reduction Agency
- Biotechnology and Biological Sciences Research Council (BB/I02643X/1)
- Australian Research Council (DP120104837)
- National Heart, Lung, and Blood Institute (HL-099073, R01HL099073)
- National Institute of General Medical Sciences (R01GM095906, GM095906)
- National Institute of Allergy and Infectious Diseases (R01AI081534, AI081534)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR057837, R01AR057837)
- National Institute of Biomedical Imaging and Bioengineering (EB02597)
- National Institute of Dental and Craniofacial Research (DE021468, R01DE021468)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- gelatin methacryloyl, cell-laden hydrogels, 3D organoids, printability, hydrogel mechanical properties, HepG2 cells, cell viability, tissue engineering, organ printing
- MeSH terms
- Animals, Biocompatible Materials, Cell Survival, Gelatin, Humans, Methacrylates, Hydrogels, Tissue Engineering, NIH 3T3 Cells, Mice, Tissue Scaffolds, Elastic Modulus, Hep G2 Cells, Bioprinting
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