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Cited by 19Bone Tissue Engineering MaterialsCalcium Carbonate Crystallization and Inhibition3D Printing in Biomedical Research

Polydopamine regulated hydroxyapatite microspheres grown in the three-dimensional honeycomb-like mollusk shell-derived organic template for osteogenesis

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Zhang, Meng, Zhang, Jiting, Ban, Lina, Qiu, Lingxi, Chen, Jisheng, Zhu, Zhihong, Wan, Ying·2020·Biofabrication
DOI 10.1088/1758-5090/ab8f20
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Abstract

Layered osteochondral composite scaffolds are considered as a promising strategy for the treatment of osteochondral defects. However, the insufficient osseous support and integration of the subchondral bone layer frequently result in the failure of osteochondral repair. Therefore, it is essentially important to explore new subchondral bone constructs tailored to support bone integration and healing. In this study, a novel three-dimensional porous biomimetic construct (HA/pDA-OTMS) was successfully developed by polydopamine (pDA) regulating hydroxyapatite (HA) microspheres grown in the honeycomb-like mollusk shell-derived organic template (OTMS). The biomimetic OTMS had good mechanical properties, high toughness, biodegradability and excellent biocompatibility. Moreover, the long-range ordered cavity structure of OTMS allowed the smallest material to create the largest and most stable geometric space, endowing it high HA loading capacity. The modification of pDA on OTMS surface effectively promoted the mineral nucleation of HA in the micro-nano cavities of OTMS. The compression mechanical tests showed that the combination of inorganic HA and organic pDA-OTMS greatly improved the mechanical properties of the construct. Additionally, the HA/pDA-OTMS composite provided adequate 3-D support for osteoblast cell attachment, proliferation and differentiation, as well as significantly up-regulated the expression of osteogenesis-related genes. These results demonstrated that as-prepared HA/pDA-OTMS constructs with combined mechanical strength and excellent osteogenic activity show great application prospects in subchondral bone regeneration.

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  • HA/PDA-OTMS showed elevated levels of osteogenesis-related genes ALP (~2-fold), OCN (~4.5-fold), and collagen type-1 (~4-fold) after 30 days of culture.

    “It also showed the elevated levels of osteogenesis-related genes such as ALP (~2-fold), OCN (~4.5-fold), collagen type-1 (~4-fold) after 30 days of culture.”

    From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [74]

    Section 3.7 reports significantly higher ALP, OCN, and Col-1 mRNA levels in HA/pDA-OTMS groups than in pure OTMS, but does not give the claimed fold changes. Section 2.12 says the cells were cultured for 7 days before gene-expression testing, not 30 days; “30d HA/pDA-OTMS” names a sample group. Page numbers are not available in our copy. (LLM, conf=0.91)

    Experimental — accuracy not yet measured

  • Immobilization of cellular growth factors within mineral deposit coatings could provide prolonged delivery of these biomolecules, thus supporting bone tissue regeneration.

    “Moreover, it was underscored that immobilization of cellular growth factors within the mineral deposit coatings could provide prolonged delivery of these biomolecules, thus supporting bone tissue regeneration [74].”

    From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [74]

    Section 3.2 reports that the mineralized HA/pDA-OTMS material adsorbed more of a model protein, BSA, and the Discussion links the constructs to bone healing. Neither section reports immobilizing cellular growth factors within mineral deposits or their prolonged delivery; the protein assay measured adsorption, not release. Page numbers were not available in our copy. (LLM, conf=0.79)

    Experimental — accuracy not yet measured

  • The HA/PDA-OTMS 3D-construct composite offered suitable 3D support for osteoblast cell attachment, proliferation, and differentiation.

    “This 3D-construct composite offered suitable 3D support for osteoblast cell attachment, proliferation, and differentiation.”

    From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [74]

  • Biomimetic OTMS allowed the smallest material to generate a stable geometric space, providing elevated HA loading capacity.

    “Due to excellent biocompatibility, this biomimetic OTMS allowed the smallest material to generate a stable geometric space, providing elevated HA loading capacity.”

    From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [74]

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Cited by

Sorted by citations

Authors
Zhang, M., Zhang, J., Ban, L., Qiu, L., Chen, J., Zhu, Z., & Wan, Y.
Affiliations
  • Central China Normal University (CN)
  • Huazhong University of Science and Technology (CN)
Corresponding author
  • Zhu, Zhihong
  • Wan, Ying
ORCID
Venue
Biofabrication
Publisher
IOP Publishing
Published
2020-06-09
Volume
12
Issue
3
Pages
035022
ISSN
  • 1758-5082 (ISSN-L)
  • 1758-5090
DOI
10.1088/1758-5090/ab8f20
OpenAlex
W3020816438
Cited by
19
References
4
Citations by year
2026: 2 · 2025: 3 · 2024: 5 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 1
FWCI
0.71
Type
journal article
Language
en
Open access
Licence
Locations
  • Biofabrication (publishedVersion)
  • PubMed (publishedVersion)
Funders
the National Key R&D Program of China (2017YFC1103800)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
polydopamine, hydroxyapatite microspheres, biomimetic scaffolds, bone formation, subchondral bone regeneration, osteochondral defects, mineral nucleation, osteogenic differentiation, mechanical properties
MeSH terms
Adsorption, Alkaline Phosphatase, Animals, Cell Line, Cell Survival, Indoles, Microspheres, Mollusca, Osteogenesis, Polymers, X-Ray Diffraction, Spectroscopy, Fourier Transform Infrared, Durapatite, Cell Shape, Mice, Animal Shells

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