Polydopamine-Templated Hydroxyapatite Reinforced Polycaprolactone Composite Nanofibers with Enhanced Cytocompatibility and Osteogenesis for Bone Tissue Engineering
Abstract
Nanohydroxyapatite (HA) synthesized by biomimetic strategy is a promising nanomaterial as bone substitute due to its physicochemical features similar to those of natural nanocrystal in bone tissue. Inspired by mussel adhesive chemistry, a novel nano-HA was synthesized in our work by employing polydopamine (pDA) as template under weak alkaline condition. Subsequently, the as-prepared pDA-templated HA (tHA) was introduced into polycaprolactone (PCL) matrix via coelectrospinning, and a bioactive tHA/PCL composite nanofiber scaffold was developed targeted at bone regeneration application. Our research showed that tHA reinforced PCL composite nanofibers exhibited favorable cytocompatibility at given concentration of tHA (0-10 w.t%). Compared to pure PCL and traditional nano-HA enriched PCL (HA/PCL) composite nanofibers, enhanced cell adhesion, spreading and proliferation of human mesenchymal stem cells (hMSCs) were observed on tHA/PCL composite nanofibers on account of the contribution of pDA present in tHA. More importantly, tHA nanoparticles exposed on the surface of composite nanofibers could further promote osteogenesis of hMSCs in vitro even in the absence of osteogenesis soluble inducing factors when compared to traditional HA/PCL scaffolds, which was supported by in vivo test as well according to the histological analysis. Overall, our study demonstrated that the developed tHA/PCL composite nanofibers with enhanced cytocompatibility and osteogenic capacity hold great potent…
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PDA coating on Ti offers strong corrosive resistance.
“which can offer the biosubstitute a strong corrosive resistance, an improved mechanical properties, and a high superficial energy [87].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [87]
PDA coating on Ti offers improved mechanical properties.
“which can offer the biosubstitute a strong corrosive resistance, an improved mechanical properties, and a high superficial energy [87].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [87]
PDA coating on Ti offers high superficial energy.
“which can offer the biosubstitute a strong corrosive resistance, an improved mechanical properties, and a high superficial energy [87].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [87]
Once Ti is coated by a PDA layer, a special strong charge transfer interaction is formed between the PDA coating layer and Ti.
“Once it is coated by the PDA layer, a special strong charge transfer interaction would be formed between the formed PDA coating layer and Ti”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [87]
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- Authors
- Gao, X., Song, J., Ji, P., Zhang, X., Li, X., Xu, X., Wang, M., Zhang, S., Deng, Y., Deng, F., & Wei, S.
- Affiliations
- Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
- Peking University (CN)
- Chongqing Medical University (CN)
- Corresponding author
- Deng, Feng
- Wei, Shicheng
- ORCID
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-01-26
- Volume
- 8
- Issue
- 5
- Pages
- 3499-3515
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2235206546
- Cited by
- 170
- References
- 65
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 14 · 2023: 13 · 2022: 17 · 2021: 23 · 2020: 25 · 2019: 24 · 2018: 17 · 2017: 17 · 2016: 7
- FWCI
- 10.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81271183, 81371697, 81570979)
- Outstanding Doctoral Scientific Research Fund, Chongqing Medical University
- Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education
- Program for Innovation Team Building at Institutions of Higher Education in Chongqing
- Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University (ODBS-2014-001)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Keywords
- hydroxyapatite nanoparticles, polycaprolactone, composite nanofibers, bone tissue engineering, bone formation, human mesenchymal stem cells, cytocompatibility, biomimetic synthesis, mussel-inspired materials, bone regeneration, scaffolds
- MeSH terms
- Biocompatible Materials, Bone Regeneration, Cell Adhesion, Cell Differentiation, Cells, Cultured, Humans, Indoles, Osteogenesis, Polyesters, Polymers, Durapatite, Tissue Engineering, Nanocomposites, Nanofibers, Mesenchymal Stem Cells
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