Punica granatum Protects Against Oxidative Stress in PC12 Cells and Oxidative Stress-Induced Alzheimer's Symptoms in Mice
Abstract
Alzheimer's disease (AD) is a progressive degenerative brain disorder that is characterized by neuronal loss, neurofibrillary tangles, and the abnormal deposition of senile plaque and amyloid β peptide (Aβ). The brains of AD patients are under intense oxidative stress. The overproduction of Aβ leads to Aβ-associated free radical oxidative stress. In this study, the antioxidative and neuronal protective effects of Punica granatum extract were investigated against oxidative stress-induced cytotoxicity in PC12 cells. The ethanol extracts of P. granatum protected PC12 cells from hydrogen peroxide (H₂O₂-induced oxidative stress. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assays revealed a significant increase in cell viability when oxidatively stressed PC12 cells were treated with the P. granatum extract. To examine the effects of P. granatum on Aβ₁₋₄₂-induced learning and memory impairment in mice, in vivo behavioral tests were performed. Treatment with the extract of P. granatum increased step-through latency in mice injected with Aβ₁₋₄₂. The results of this study suggest that the ethanol extract of P. granatum mitigated H₂O₂-induced oxidative stress in PC12 cells. In addition, the extract inhibited neuronal cell death caused by Aβ-induced oxidative stress and Aβ-induced learning and memory deficiency.
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Juice and extracts from Punica granatum have shown neuroprotective effects in animal models, potentially by counteracting oxidative damage, minimizing inflammation of the brain and soluble Aβ42 and hippocampal amyloid deposition.
“Likewise, juice and extracts from Punica granatum have also shown neuroprotective effects in animal models, potentially by counteracting oxidative damage, minimizing inflammation of the brain and soluble Aβ 42 and hippocampal amyloid deposition [182,183].”
From The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment (2022) · cites this paper as [182]
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“Likewise, juice and extracts from Punica granatum have also shown neuroprotective effects in animal models, potentially by counteracting oxidative damage, minimizing inflammation of the brain and soluble Aβ 42 and hippocampal amyloid deposition [182,183].”
Background
- Authors
- Choi, S. J., Lee, J.-H., Heo, H. J., Cho, H. Y., Kim, H. K., Kim, C.-J., Kim, M. O., Suh, S. H., & Shin, D.-H.
- Affiliations
- North Carolina State University (US)
- Korea University (KR)
- Gyeongsang National University (KR)
- Hanseo University (KR)
- Kyung Hee University (KR)
- Corresponding author
- Shin, Dong-Hoon
- Venue
- Journal of Medicinal Food
- Publisher
- SAGE Publications
- Published
- 2011-07
- Volume
- 14
- Issue
- 7-8
- Pages
- 695-701
- ISSN
- 1096-620X (ISSN-L)
- 1557-7600
- OpenAlex
- W2000610936
- Cited by
- 57
- References
- 41
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 10 · 2021: 6 · 2020: 3 · 2019: 1 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 6 · 2012: 2
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medicinal Food (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Keywords
- Punica granatum, pomegranate extract, Alzheimer's disease, oxidative stress, amyloid-beta, hydrogen peroxide, PC12 cells, neuronal cell death, learning and memory impairment, neuroprotection, antioxidant activity
- MeSH terms
- Alzheimer Disease, Animals, Antioxidants, Cell Survival, Disease Models, Animal, Hydrogen Peroxide, Male, Memory, Mice, Inbred ICR, Neurons, Plant Extracts, Amyloid beta-Peptides, PC12 Cells, Oxidative Stress, Protective Agents, Lythraceae, Mice, Rats
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