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Electrospun Silk Fibroin Scaffolds with Macropores for Bone Regeneration: An In Vitro and In Vivo Study
Abstract
We developed three-dimensional electrospun silk fibroin (ESF) scaffolds with controllable pore size. The purpose of this study was to evaluate ESF scaffolds with pores (P-ESF) for bone regeneration via in vitro and in vivo studies, with a comparison to a commercially available porous three-dimensional polylactic acid (PLA) scaffold. P-ESF supported significantly higher proliferation and alkaline phosphatase activity of osteoblasts than PLA in vitro (p < 0.05). Moreover, higher expression levels of activated adhesion-related proteins, including focal adhesion kinase, were observed in the P-ESF than in PLA, as confirmed by western blot analyses. Microcomputed tomography revealed that 78.30% of the original bone volume was attained in the P-ESF implantation group at 7 weeks after critical bone defect formation in rat calvaria. Comparatively, the PLA implantation group showed only 49.31%. Histological evaluation also showed new bone tissue formation upon P-ESF implantation. Taken together, the P-ESF scaffold may be a good bone substitute for bone regeneration.
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“The researchers used the three-dimensional porous scaffolds of silk fibroin prepared by electrostatic spinning and three-dimensional PLA scaffolds to carry out experimental comparative studies, and the results showed that the silk fibroin scaffolds could significantly promote the ALP activity and osteogenic differentiation ability of mouse embryonic osteoblast precursor cells (MC3T3-E1);”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [75]
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“moreover, the silk fibroin scaffolds were significantly superior to the PLA scaffolds in terms of the ability to form new bone in the animal experiments [75], which suggests that electrostatically spun silk fibroin scaffolds have good potential for bone tissue repair.”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [75]
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Metadata
- Authors
- Park, S. Y., Ki, C. S., Park, Y. H., Jung, H. M., Woo, K. M., & Kim, H. J.
- Affiliations
- Seoul National University (KR)
- Corresponding author
- Kim, Hyun Jeong
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2010-04
- Volume
- 16
- Issue
- 4
- Pages
- 1271-1279
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- OpenAlex
- W2066288600
- Cited by
- 120
- References
- 44
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 5 · 2023: 2 · 2022: 6 · 2021: 7 · 2020: 8 · 2019: 11 · 2018: 7 · 2017: 9 · 2016: 12 · 2015: 10 · 2014: 11 · 2013: 5 · 2012: 7
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Tissue Engineering Part A (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Funders
- Korea Science and Engineering Foundation
- European Social Fund
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- silk fibroin, electrospun scaffolds, macropores, bone regeneration, bone substitutes, polylactic acid scaffolds, osteoblast proliferation, alkaline phosphatase activity, focal adhesion kinase, critical-size calvarial defect, new bone formation, pore size control
- MeSH terms
- Animals, Biocompatible Materials, Bone Regeneration, Fibroins, Male, Materials Testing, Microscopy, Electron, Scanning, Polyesters, Polymers, Radiography, Bombyx, Skull, 3T3 Cells, Rats, Sprague-Dawley, Lactic Acid, Tissue Engineering, Mice, Rats, Tissue Scaffolds
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