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Cited by 349

The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.

Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum.

Open access
Stirling, C J, Rothblatt, J, Hosobuchi, M, Deshaies, R, Schekman, R·1992·Molecular Biology of the Cell
DOI 10.1091/mbc.3.2.129
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Abstract

Yeast mutants defective in the translocation of soluble secretory proteins into the lumen of the endoplasmic reticulum (sec61, sec62, sec63) are not impaired in the assembly and glycosylation of the type II membrane protein dipeptidylaminopeptidase B (DPAPB) or of a chimeric membrane protein consisting of the multiple membrane-spanning domain of yeast hydroxymethylglutaryl CoA reductase (HMG1) fused to yeast histidinol dehydrogenase (HIS4C). This chimera is assembled in wild-type or mutant cells such that the His4c protein is oriented to the ER lumen and thus is not available for conversion of cytosolic histidinol to histidine. Cells harboring the chimera have been used to select new translocation defective sec mutants. Temperature-sensitive lethal mutations defining two complementation groups have been isolated: a new allele of sec61 and a single isolate of a new gene sec65. The new isolates are defective in the assembly of DPAPB, as well as the secretory protein alpha-factor precursor. Thus, the chimeric membrane protein allows the selection of more restrictive sec mutations rather than defining genes that are required only for membrane protein assembly. The SEC61 gene was cloned, sequenced, and used to raise polyclonal antiserum that detected the Sec61 protein. The gene encodes a 53-kDa protein with five to eight potential membrane-spanning domains, and Sec61p antiserum detects an integral protein localized to the endoplasmic reticulum membrane. Sec61p appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum.

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Metadata

Authors
Stirling, C. J., Rothblatt, J., Hosobuchi, M., Deshaies, R., & Schekman, R.
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
1992-02
Volume
3
Issue
2
Pages
129-142
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.3.2.129
OpenAlex
W2009168935
Cited by
349
References
37
FWCI
11.54
Type
journal article
Language
en
Open access
green

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