Cited by 53Endometriosis Research and TreatmentAutophagy in Disease and TherapyEndometrial and Cervical Cancer Treatments

Autophagy contributes to hypoxia-induced epithelial to mesenchymal transition of endometrial epithelial cells in endometriosis

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Liu, Hengwei, Du, Yu, Zhang, Zhibing, Lv, Liqun, Xiong, Wenqian, Zhang, Ling, Li, Na, He, Haitang +2 more·2018·Biology of Reproduction

Abstract

Endometriosis is a benign gynecologic disorder, and presents with malignant characteristics, such as migration and invasion. Hypoxia has been implicated in triggering epithelial-mesenchymal transition (EMT). Hypoxia is also known to induce autophagy. However, the relationship between autophagy and EMT under hypoxia conditions in endometriosis remains unknown. In the present study, we found that the expression of hypoxia-inducible factor-1α (HIF-1α), microtubule associated protein light chain 3 (LC3), and mesenchymal cell marker vimentin was significantly higher in ectopic endometrium from patients with endometriosis, along with decreased expression of epithelial cell marker E-cadherin. After hypoxia treatment, endometrial epithelial cells exhibited enhanced migration and invasion abilities, as well as promoted autophagy and the EMT phenotype. Our analyses also show that HIF-1α was responsible for induction of autophagy. Moreover, inhibition of autophagy by chemical or genetic approaches suppressed hypoxia triggered EMT and reduced cell migration and invasion. Collectively, our findings identify that autophagy is critical for the migration and invasion of endometrial cells through the induction of EMT and indicate that inhibition of autophagy may be a novel useful strategy in the treatment of endometriosis.

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  • In endometrial epithelial cells, hypoxia-induced EMT is facilitated by enhanced autophagy.

    “In endometrial epithelial cells, the hypoxia-induced EMT is facilitated by enhanced autophagy [95].”

    From The Pathogenesis of Adenomyosis vis-à-vis Endometriosis (2020) · cites this paper as [95]

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  1. [3]Bassi MA, Podgaec S, Dias Junior JA, Sobrado CW, N DAF. Bowel endometriosis: a benign disease?. Rev Assoc Med Bras 2009; 55:611–616. [DOI] [PubMed] [Google Scholar]

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Authors
Liu, H., Du, Y., Zhang, Z., Lv, L., Xiong, W., Zhang, L., Li, N., He, H., Li, Q., & Liu, Y.
Affiliations
  • Wuhan Union Hospital (CN)
  • Virginia Commonwealth University (US)
  • Department of Obstetrics and Gynecology, Wu Han Kang Jian Fu Ying Hospital, Wuhan, China
Corresponding author
Liu, Yi
Venue
Biology of Reproduction
Published
2018-05-31
Volume
99
Issue
5
Pages
968-981
ISSN
  • 0006-3363 (ISSN-L)
  • 1529-7268
OpenAlex
W2807648383
Cited by
53
References
50
Citations by year
2026: 4 · 2025: 7 · 2024: 5 · 2023: 6 · 2022: 10 · 2021: 4 · 2020: 9 · 2019: 5 · 2018: 3
FWCI
5.63
Type
journal article
Language
en
Open access
Locations
  • Biology of Reproduction (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81471439)
  • National Institutes of Health (HD076257)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Health Sciences › Medicine › Obstetrics and Gynecology › Endometrial and Cervical Cancer Treatments
Keywords
endometriosis, autophagy, epithelial-mesenchymal transition, hypoxia, HIF-1α, endometrial epithelial cells, cell migration, cell invasion, E-cadherin, vimentin, LC3, autophagy inhibition
MeSH terms
S100 Calcium-Binding Protein A4, Adult, Hypoxia, Autophagy, Cell Line, Cell Movement, Endometriosis, Endometrium, Epithelial Cells, Female, Humans, Microtubule-Associated Proteins, Middle Aged, Vimentin, Cadherins, Hypoxia-Inducible Factor 1, alpha Subunit, Epithelial-Mesenchymal Transition

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