Cited by 216Endometriosis Research and TreatmentEctopic Pregnancy Diagnosis and ManagementPlant and fungal interactions

Epithelial to mesenchymal transition-like and mesenchymal to epithelial transition-like processes might be involved in the pathogenesis of pelvic endometriosis†

Matsuzaki, Sachiko, Darcha, Claude·2012·Human Reproduction

Abstract

BACKGROUND: Endometrium is derived from intermediate mesoderm via mesenchymal to epithelial transition (MET) during development of the urogenital system. By retaining some imprint of their mesenchymal origin, endometrial epithelial cells may be particularly prone to return to this state, via epithelial to mesenchymal transition (EMT). We hypothesized that pelvic endometriosis originates from retrograde menstruation of endometrial tissue and that EMT-like and MET-like processes might be involved in the pathogenesis of pelvic endometriosis. METHODS: We investigated commonly used molecular markers for EMT, including cytokeratin, E-cadherin, N-cadherin, vimentin, S100A4 and dephosphorylated beta-catenin by immunohistochemistry in different forms of pelvic endometriosis: deep infiltrating endometriosis, ovarian endometriosis and superficial peritoneal endometriosis (red and black lesions), as well as samples of menstrual endometrium, other benign ovarian cysts (mucinous and serous cyst adenoma), and abdominal scar endometriosis for comparison. RESULTS: Epithelial cells of red peritoneal lesions and ovarian endometriosis showed less epithelial marker (cytokeratin, P < 0.0001) expression and more mesenchymal marker (vimentin and/or S100A4, P < 0.0001) expression than those of menstrual endometrium. In contrast, epithelial cells of black peritoneal lesions and deep infiltrating endometriosis showed more epithelial marker (E-cadherin) expression than those of menstrual endometrium (P < 0.03), red peritoneal lesions (P < 0.0001) and ovarian endometriosis (P< 0.0001), but maintained expression of some mesenchymal markers (vimentin, S100A4). In addition, dephosphorylated beta-catenin protein expression was significantly higher in epithelial cells of deep infiltrating endometriosis (P < 0.0001) than in epithelial cells of red and black peritoneal lesions and ovarian endometriosis. CONCLUSIONS: EMT-like and MET-like processes might be involved in the pathogenesis of pelvic endometriosis.

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Crossref · 10.1093/humrep/der442

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Authors
Matsuzaki, S., & Darcha, C.
Affiliations
Centre Hospitalier Universitaire de Clermont-Ferrand (FR)
Venue
Human Reproduction
Published
2012-01-02
Volume
27
Issue
3
Pages
712-721
ISSN
  • 0268-1161 (ISSN-L)
  • 1460-2350
OpenAlex
W2171875732
Cited by
216
References
42
Citations by year
2026: 5 · 2025: 17 · 2024: 14 · 2023: 15 · 2022: 16 · 2021: 23 · 2020: 23 · 2019: 13 · 2018: 21 · 2017: 18 · 2016: 14 · 2015: 16 · 2014: 10 · 2013: 8 · 2012: 3
FWCI
13.83
Type
journal article
Language
en
Open access
closed
Locations
  • Human Reproduction (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
Keywords
epithelial-mesenchymal transition, mesenchymal-epithelial transition, endometriosis pathogenesis, retrograde menstruation, deep infiltrating endometriosis, endometrioma, peritoneal endometriosis, E-cadherin, N-cadherin, vimentin, S100A4, cytokeratin, endometrial epithelial cells
MeSH terms
S100 Calcium-Binding Protein A4, Adult, Cell Differentiation, Cicatrix, Endometriosis, Epithelial Cells, Female, Humans, Keratins, Menstrual Cycle, S100 Proteins, Ovarian Cysts, Vimentin, Biomarkers, Cadherins, beta Catenin, Epithelial-Mesenchymal Transition

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