The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Radioimmunoassay of leptin in human plasma
Abstract
Recent studies suggest that leptin, the ob gene product absent in ob/ob mice, is a negative regulator of adiposity. We developed an RIA to measure human leptin in plasma or serum. The minimum detectable concentration by the assay is 0.5 microg/L leptin and the limit of linearity is 100 microg/L. Recovery of leptin added to serum was 99-104% over by the linear range of the assay. The RIA agreed reasonably well with rough quantification by Western blot (RIA = 0.90 blot + 3.7 microg/L, Sy/x = 10.9 microg/L). CVs within- and between-run ranged from 3.4% to 8.3% and from 3.6% to 6.2%, respectively. Variation in plasma leptin concentrations in specimens collected on consecutive mornings was large (CVs of 10.9% and 22.5%). After an overnight fast, leptin concentrations were similar to those 1-2 h after 1-2 meals. Plasma leptin concentrations in specimens from 83 lean and obese adults correlated directly with body mass index (BMI; kg/m2): r = 0.72, P <0.001. Correlations were significantly improved by separating results by gender (men r = 0.84, women r = 0.87; p <0.001). The increase in leptin concentrations with increasing BMI was greater in women than in men (slope 2.53 vs 0.97 microg/L per unit BMI, respectively). Leptin concentrations determined in lean subjects (BMI between 18 and 25) were higher in women (7.36 +/- 3.73 microg/L) than in men (3.84 +/- 1.79 microg/L) (P <0.001). Plasma leptin varied little with age and no significant difference was observed between whites and blacks. We conclude that: (a) plasma leptin concentrations are accurately and precisely measured by this new RIA; (b) leptin concentrations vary little due to short-term fasting, age, or race; but (c) plasma leptin concentrations are gender specific.
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Cites
Positional cloning of the mouse obese gene and its human homologue
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Serum Immunoreactive-Leptin Concentrations in Normal-Weight and Obese Humans
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Weight-Reducing Effects of the Plasma Protein Encoded by the obese Gene
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Identification and expression cloning of a leptin receptor, OB-R.
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Cited by
Cnop, M., Havel, P. J., Utzschneider, K. M., Carr, D. B., Sinha, M. K., Boyko, E. J., Retzlaff, B. M., Knopp, R. H., Brunzell, J. D., & Kahn, S. E. (2003) · Diabetologia · doi:10.1007/s00125-003-1074-z · Cited by 1,483
Cnop, M., Landchild, M. J., Vidal, J., Havel, P. J., Knowles, N. G., Carr, D. R., Wang, F., Hull, R. L., Boyko, E. J., Retzlaff, B. M., et al. (2002) · Diabetes · doi:10.2337/diabetes.51.4.1005 · Cited by 432
Association of Plasma Leptin Levels With Incident Alzheimer Disease and MRI Measures of Brain Aging
Lieb, W. (2009) · JAMA · doi:10.1001/jama.2009.1836 · Cited by 428
Serum Leptin and Vascular Risk Factors in Obstructive Sleep Apnea
Ip, M. S. M., Lam, K. S. L., Ho, C.-M., Tsang, K. W. T., & Lam, W.-K. (2000) · Chest · doi:10.1378/chest.118.3.580 · Cited by 420
Serum Vaspin Concentrations in Human Obesity and Type 2 Diabetes
Youn, B.-S., Klöting, N., Kratzsch, J., Lee, N., Park, J. W., Song, E.-S., Ruschke, K., Oberbach, A., Fasshauer, M., Stumvoll, M., et al. (2008) · Diabetes · doi:10.2337/db07-1045 · Cited by 419
Metadata
- Authors
- Ma, Z., Gingerich, R. L., Santiago, J. V., Klein, S., Smith, C. H., & Landt, M.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-06-01
- Volume
- 42
- Issue
- 6
- Pages
- 942-946
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2167628665
- Cited by
- 494
- References
- 14
- FWCI
- 16.68
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ma, Z., Gingerich, R. L., Santiago, J. V., Klein, S., Smith, C. H., & Landt, M. (1996). Radioimmunoassay of leptin in human plasma. Clinical Chemistry. https://doi.org/10.1093/clinchem/42.6.942
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