The fitness costs and benefits of trisomy of each Candida albicans chromosome
Open accessPublished version of a preprint
Abstract
Candida albicans is a prevalent human fungal pathogen. Rapid genomic change, due to aneuploidy, is a common mechanism that facilitates survival from multiple types of stresses including the few classes of available antifungal drugs. The stress survival of aneuploids occurs despite the fitness costs attributed to most aneuploids growing under idealized lab conditions. Systematic study of the aneuploid state in C. albicans has been hindered by the lack of a comprehensive collection of aneuploid strains. Here, we describe a collection of diploid C. albicans aneuploid strains, each carrying one extra copy of each chromosome, all from the same genetic background. We tested the fitness of this collection under several physiological conditions including shifts in pH, low glucose, oxidative stress, temperature, high osmolarity, membrane stress, and cell wall stress. We found that most aneuploids, under most conditions, were less fit than their euploid parent, yet there were specific conditions under which specific aneuploid isolates provided a fitness benefit relative to the euploid parent strain. Importantly, this fitness benefit was attributable to the change in the copy number of specific chromosomes. Thus, C. albicans can tolerate aneuploidy of each chromosome and some aneuploids confer improved growth under conditions that the yeast encounters in its host niches.
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DNA-seq library preparation was performed as described previously.
“Total DNA extraction and genomic DNA library preparation were performed as described previously (Yang et al., 2021).”
From Miconazole induces aneuploidy-mediated tolerance in Candida albicans that is dependent on Hsp90 and calcineurin (2024) · cites this paper as Yang et al., 2021
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- Authors
- Yang, F., Todd, R. T., Selmecki, A., Jiang, Y.-Y., Cao, Y.-B., & Berman, J.
- Affiliations
- Tongji University (CN)
- University of Minnesota (US)
- Shanghai University of Traditional Chinese Medicine (CN)
- Tel Aviv University (IL)
- Corresponding author
- Berman, Judith
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2021-04-09
- Volume
- 218
- Issue
- 2
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- PMC
- PMC8225349
- OpenAlex
- W3144914243
- Cited by
- 72
- References
- 35
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 25 · 2023: 14 · 2022: 9 · 2021: 5
- FWCI
- 4.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (82020108032, 81673478, 81872910)
- Shanghai Key Basic Research Project (19JC1414900)
- National Institute of Health (R01AI143689)
- Burroughs Wellcome Fund Investigators in the Pathogenesis of Infectious Diseases (1020388)
- Israel Science foundation (997/18)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida albicans, aneuploidy, trisomy, fitness cost, fitness benefits, chromosome copy number, stress tolerance, antifungal resistance, osmotic stress, oxidative stress, pH stress, glucose limitation, temperature stress, membrane stress, cell wall stress, fungal pathogens
- MeSH terms
- Host Microbial Interactions, Antifungal Agents, Candida albicans, Candidiasis, Humans, Trisomy, Chromosomes, Fungal, Genome, Fungal, Drug Resistance, Fungal, Genetic Fitness
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