Tunicamycin Potentiates Antifungal Drug Tolerance via Aneuploidy in Candida albicans
Open accessAbstract
Candida albicans is a prevalent human fungal commensal and also a pathogen that causes life-threatening systemic infections. Treatment failures are frequent because few therapeutic antifungal drug classes are available and because drug resistance and tolerance limit drug efficacy.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Caspofungin heteroresistance in C. glabrata is in sharp contrast to the mechanism described for C. albicans and C. neoformans heteroresistance, where aneuploidy is a common driver.
“This finding positions caspofungin heteroresistance in C. glabrata in sharp contrast to the mechanism described for C. albicans and C. neoformans heteroresistance, where aneuploidy is a common driver [36, 37].”
From Calcineurin‐Dependent Stress Adaptation Enables Caspofungin Heteroresistance Leading to Stable Resistance in <i>Candida Glabrata</i> (2026) · cites this paper as [36]
The Introduction (page not specified) says aneuploidy enables adaptation to caspofungin in C. albicans and to fluconazole and flucytosine in C. neoformans. It supports aneuploidy as an antifungal-adaptation mechanism in both species, but does not identify heteroresistance as the mechanism in either or establish a contrast with C. glabrata caspofungin heteroresistance. (LLM, conf=0.83)
Experimental — accuracy not yet measuredIn C. albicans, TUN induces amplification of chromosome 2, resulting in upregulation of genes associated with caspofungin tolerance, potentiating cross-tolerance to TUN and caspofungin.
“In the diploid fungal pathogen Candida albicans, TUN induces amplification of chromosome 2, which results in the upregulation of several genes associated with tolerance to caspofungin, the first-line antifungal drug. This genetic adaptation potentiates cross-tolerance to both TUN and caspofungin (Yang et al., 2021).”
From Uncovering the connection between tunicamycin-induced respiratory deficiency and reduced fluconazole tolerance in Candida glabrata (2025) · cites this paper as Yang et al., 2021
The Introduction reports that tunicamycin exposure yielded chromosome 2 duplications and that trisomy enabled tolerance to both tunicamycin and caspofungin. However, it identifies only one chromosome 2 gene, MKK2, as associated with tolerance to both drugs; the cited passages do not establish upregulation of several caspofungin-tolerance genes as the mechanism. (LLM, conf=0.86)
Experimental — accuracy not yet measuredAzole tolerance in Candida is typically temperature-independent and frequently associated with aneuploidy, which may be lost upon temperature changes or ploidy recovery.
“This tolerance is typically temperature-independent and frequently associated with aneuploidy, which may be lost upon temperature changes or ploidy recovery [114,115].”
From Insight into the Mechanisms and Clinical Relevance of Antifungal Heteroresistance (2025) · cites this paper as [115]
The Introduction associates aneuploidy with antifungal tolerance and fluconazole adaptation in C. albicans, and the Discussion describes aneuploidy as a reversible stress response (page unavailable in our copy). Neither passage establishes that azole tolerance is typically temperature-independent or that it is lost after temperature changes or ploidy recovery. The Results also caution that the aneuploid strains studied were more susceptible to fluconazole, so the paper does not establish a general association between aneuploidy and azole tolerance. (LLM, conf=0.84)
Experimental — accuracy not yet measuredExposure to hydroxyurea or tunicamycin selects for trisomy of chromosome 2 in C. albicans, which also enables tolerance to caspofungin.
“Recent work in C. albicans demonstrated that exposure to the cancer chemotherapeutic hydroxyurea, or the ER stress-inducing agent tunicamycin selects for trisomy of chromosome 2, which also enables tolerance to the echinocandin caspofungin66,67.”
From Molecular mechanisms governing antifungal drug resistance (2023) · cites this paper as [67]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
seaborn: statistical data visualization
Waskom, M. (2021) · Journal of Open Source Software · doi:10.21105/joss.03021 · Cited by 7,310 · see citing works
Pappas, P. G., Kauffman, C. A., Andes, D. R., Clancy, C. J., Marr, K. A., Ostrosky-Zeichner, L., Reboli, A. C., Schuster, M. G., Vazquez, J. A., Walsh, T. J., et al. (2016) · Clinical Infectious Diseases · doi:10.1093/cid/civ933 · Cited by 4,200 · see citing works
Effects of Aneuploidy on Cellular Physiology and Cell Division in Haploid Yeast
Torres, E. M., Sokolsky, T., Tucker, C. M., Chan, L. Y., Boselli, M., Dunham, M. J., & Amon, A. (2007) · Science · doi:10.1126/science.1142210 · Cited by 935 · see citing works
From acute ER stress to physiological roles of the Unfolded Protein Response
Wu, J., & Kaufman, R. J. (2006) · Cell Death and Differentiation · doi:10.1038/sj.cdd.4401840 · Cited by 868 · see citing works
Levin, D. E. (2011) · Genetics · doi:10.1534/genetics.111.128264 · Cited by 827 · see citing works
Pfaller, M. A., Diekema, D. J., Turnidge, J. D., Castanheira, M., & Jones, R. N. (2019) · Open Forum Infectious Diseases · doi:10.1093/ofid/ofy358 · Cited by 825 · see citing works
Aneuploidy and Isochromosome Formation in Drug-Resistant <i>Candida albicans</i>
Selmecki, A., Forche, A., & Berman, J. (2006) · Science · doi:10.1126/science.1128242 · Cited by 733 · see citing works
Self-citationDrug resistance and tolerance in fungi.
Berman, J., & Krysan, D. J. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0322-2 · Cited by 654 · cited 3× · see citing works
“slowly, in the presence of supra-MIC drug concentrations (5, 6).”
BackgroundMethodInfluentialSelf-citationThe Endoplasmic Reticulum: Folding, Calcium Homeostasis, Signaling, and Redox Control
Görlach, A., Klappa, P., & Kietzmann, T. (2006) · Antioxidants and Redox Signaling · doi:10.1089/ars.2006.8.1391 · Cited by 616 · see citing works
Aneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast
Pavelka, N., Rancati, G., Zhu, J., Bradford, W. D., Saraf, A., Florens, L., Sanderson, B. W., Hattem, G. L., & Li, R. (2010) · Nature · doi:10.1038/nature09529 · Cited by 577 · see citing works
Sionov, E., Lee, H., Chang, Y. C., & Kwon-Chung, K. J. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1000848 · Cited by 479 · see citing works
Stingele, S., Stoehr, G., Peplowska, K., Cox, J., Mann, M., & Štorchová, Z. (2012) · Molecular Systems Biology · doi:10.1038/msb.2012.40 · Cited by 449 · see citing works
Mammalian stress response: induction of the glucose-regulated protein family
Lee, A. S. (1992) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(92)90042-b · Cited by 442 · see citing works
Treatment strategies for cryptococcal infection: challenges, advances and future outlook
Iyer, K. R., Revie, N. M., Fu, C., Robbins, N., & Cowen, L. E. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00511-0 · Cited by 363 · see citing works
Hsp90 Governs Echinocandin Resistance in the Pathogenic Yeast Candida albicans via Calcineurin
Singh, S. D., Robbins, N., Zaas, A. K., Schell, W. A., Perfect, J. R., & Cowen, L. E. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000532 · Cited by 362 · cited 1× · see citing works
“The calcineurin pathway is also required for tolerance to echinocandins (30).”
BackgroundSelmecki, A., Gerami‐Nejad, M., Paulson, C., Forche, A., & Berman, J. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06176.x · Cited by 325 · see citing works
Self-citationAgrawal, A., Singh, A. S., Verma, R., & Murari, A. (2014) · Journal of Oral and Maxillofacial Pathology · doi:10.4103/0973-029x.141325 · Cited by 300 · see citing works
Transcriptional consequences of aneuploidy
Sheltzer, J. M., Torres, E. M., Dunham, M. J., & Amon, A. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1209227109 · Cited by 290 · see citing works
Rosenberg, A., Ene, I. V., Bibi, M., Zakin, S., Segal, E. S., Ziv, N., Dahan, A. M., Colombo, A. L., Bennett, R. J., & Berman, J. (2018) · Nature Communications · doi:10.1038/s41467-018-04926-x · Cited by 270 · see citing works
Self-citationQuantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast
Dephoure, N., Hwang, S., O'Sullivan, C., Dodgson, S. E., Gygi, S. P., Amon, A., & Torres, E. M. (2014) · eLife · doi:10.7554/elife.03023 · Cited by 263 · see citing works
Cellular Stress Associated with Aneuploidy
Zhu, J., Tsai, H.-J., Gordon, M. R., & Li, R. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.02.002 · Cited by 221 · see citing works
Formation of new chromosomes as a virulence mechanism in yeast <i>Candida glabrata</i>
Poláková, S., Blume, C., Zárate, J. Á., Mentel, M., Jørck-Ramberg, D., Stenderup, J., & Piškur, J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0809793106 · Cited by 206 · see citing works
Janbon, G., Sherman, F., & Rustchenko, E. P. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.9.5150 · Cited by 202 · see citing works
Dumetz, F., Imamura, H., Sanders, M. J., Šeblová, V., Myšková, J., Pescher, P., Vanaerschot, M., Meehan, C. J., Cuypers, B., Muylder, G. D., et al. (2017) · mBio · doi:10.1128/mbio.00599-17 · Cited by 196 · see citing works
Reinoso-Martín, C., Schüller, C., Schuetzer-Muehlbauer, M., & Kuchler, K. (2003) · Eukaryotic Cell · doi:10.1128/ec.2.6.1200-1210.2003 · Cited by 193 · see citing works
Protein Kinase Cθ Is Required for Autophagy in Response to Stress in the Endoplasmic Reticulum
Sakaki, K., Wu, J., & Kaufman, R. J. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m710209200 · Cited by 179 · see citing works
Current Antimycotics, New Prospects, and Future Approaches to Antifungal Therapy
Wall, G., & Lopez-Ribot, J. L. (2020) · Antibiotics · doi:10.3390/antibiotics9080445 · Cited by 176 · see citing works
Dynamic ploidy changes drive fluconazole resistance in human cryptococcal meningitis
Stone, N. R. H., Rhodes, J., Fisher, M. C., Mfinanga, S., Kivuyo, S., Rugemalila, J., Segal, E. S., Needleman, L., Molloy, S. F., Kwon-Chung, J., et al. (2019) · Journal of Clinical Investigation · doi:10.1172/jci124516 · Cited by 167 · see citing works
Self-citationControl of the C. albicans Cell Wall Damage Response by Transcriptional Regulator Cas5
Bruno, V. M., Kalachikov, S., Subaran, R., Nobile, C. J., Kyratsous, C., & Mitchell, A. P. (2006) · PLoS Pathogens · doi:10.1371/journal.ppat.0020021 · Cited by 157 · see citing works
Global analysis of mutations driving microevolution of a heterozygous diploid fungal pathogen
Ene, I. V., Farrer, R. A., Hirakawa, M. P., Agwamba, K., Cuomo, C. A., & Bennett, R. J. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1806002115 · Cited by 155 · cited 1× · see citing works
“, passage in mouse blood (70), the oral cavity (71, 72), and the gastrointestinal tract (73).”
BackgroundEvolution in<i>Candida albicans</i>Populations During a Single Passage Through a Mouse Host
Forche, A., Magee, P. T., Selmecki, A., Berman, J., & May, G. (2009) · Genetics · doi:10.1534/genetics.109.103325 · Cited by 147 · see citing works
Self-citationAbbey, D. A., Funt, J., Lurie-Weinberger, M. N., Thompson, D. A., Regev, A., Myers, C. L., & Berman, J. (2014) · Genome Medicine · doi:10.1186/s13073-014-0100-8 · Cited by 147 · see citing works
Self-citationSpecific Chromosome Alterations in Fluconazole-Resistant Mutants of Candida albicans
Perepnikhatka, V. I., Fischer, F. J., Niimi, M., Baker, R. A., Cannon, R. D., Wang, Y.-K., Sherman, F., & Rustchenko, E. P. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.13.4041-4049.1999 · Cited by 141 · see citing works
Longitudinal Survey of Fungi in the Human Gut: ITS Profiling, Phenotyping, and Colonization
Raimondi, S., Amaretti, A., Gozzoli, C., Simone, M., Righini, L., Candeliere, F., Brun, P., Ardizzoni, A., Colombari, B., Paulone, S., et al. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01575 · Cited by 138 · see citing works
Dosage compensation can buffer copy-number variation in wild yeast
Hose, J., Yong, C. M., Sardi, M. I., Wang, Z., Newton, M. A., & Gasch, A. P. (2015) · eLife · doi:10.7554/elife.05462 · Cited by 131 · see citing works
In vivo cellular adaptation to ER stress: survival strategies with double-edged consequences
Tsang, K. Y., Chan, D. T.-M., Bateman, J. F., & Cheah, K. S. E. (2010) · Journal of Cell Science · doi:10.1242/jcs.068833 · Cited by 129 · see citing works
Endoplasmic reticulum stress response in yeast and humans
Wu, H., Ng, B. S. H., & Thibault, G. (2014) · Bioscience Reports · doi:10.1042/bsr20140058 · Cited by 124 · see citing works
Chromosome instability in Candida albicans
Rustchenko, E. P. (2006) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2006.00150.x · Cited by 117 · see citing works
Rustchenko, E. P., Howard, D. H., & Sherman, F. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.11.3231-3241.1994 · Cited by 111 · see citing works
Aneuploidy in yeast: Segregation error or adaptation mechanism?
Gilchrist, C., & Stelkens, R. B. (2019) · Yeast · doi:10.1002/yea.3427 · Cited by 110 · see citing works
Aneuploidy increases resistance to chemotherapeutics by antagonizing cell division
Replogle, J. M., Zhou, W., Amaro, A. E., McFarland, J. M., Villalobos-Ortiz, M., Ryan, J. A., Letai, A. G., Yilmaz, Ö. H., Sheltzer, J. M., Lippard, S. J., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2009506117 · Cited by 110 · see citing works
Aneuploidy Enables Cross-Adaptation to Unrelated Drugs
Yang, F., Teoh, F., Tan, A. S. M., Cao, Y., Pavelka, N., & Berman, J. (2019) · Molecular Biology and Evolution · doi:10.1093/molbev/msz104 · Cited by 101 · see citing works
Self-citationForche, A., Cromie, G., Gerstein, A. C., Solis, N. V., Pisithkul, T., Srifa, W., Jeffery, E., Abbey, D., Filler, S. G., Dudley, A. M., et al. (2018) · Genetics · doi:10.1534/genetics.118.301019 · Cited by 94 · see citing works
Self-citationBing, J., Hu, T., Zheng, Q., Muñoz, J. F., Cuomo, C. A., & Huang, G. (2020) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01466-20 · Cited by 86 · see citing works
Yang, F., Zhang, L., Wakabayashi, H., Myers, J., Jiang, Y., Cao, Y., Jimenez-Ortigosa, C., Perlin, D. S., & Rustchenko, E. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00071-17 · Cited by 81 · see citing works
Self-citationPloidy plasticity: a rapid and reversible strategy for adaptation to stress
Berman, J. G. (2016) · FEMS Yeast Research · doi:10.1093/femsyr/fow020 · Cited by 80 · see citing works
Self-citationDosage compensation and the global re-balancing of aneuploid genomes
Prestel, M., Feller, C., & Becker, P. B. (2010) · Genome biology · doi:10.1186/gb-2010-11-8-216 · Cited by 75 · see citing works
The fitness costs and benefits of trisomy of each <i>Candida albicans</i> chromosome
Yang, F., Todd, R. T., Selmecki, A., Jiang, Y.-Y., Cao, Y.-B., & Berman, J. (2021) · Genetics · doi:10.1093/genetics/iyab056 · Cited by 72 · see citing works
Self-citationGerstein, A. C., Rosenberg, A., Hecht, I., & Berman, J. (2016) · Microbiology · doi:10.1099/mic.0.000295 · Cited by 63 · see citing works
Self-citationYang, F., Kravets, A., Bethlendy, G., Welle, S., & Rustchenko, E. (2013) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00516-13 · Cited by 60 · see citing works
Self-citationA Double-Edged Sword: Aneuploidy is a Prevalent Strategy in Fungal Adaptation
Tsai, H.-J., & Nelliat, A. (2019) · Genes · doi:10.3390/genes10100787 · Cited by 60 · see citing works
Global Analysis of Gene Expression in Response to Whole-Chromosome Aneuploidy in Hexaploid Wheat
Zhang, A., Li, N., Gong, L., Gou, X., Wang, B., Deng, X., Li, C., Dong, Q., Zhang, H., & Liu, B. (2017) · PLANT PHYSIOLOGY · doi:10.1104/pp.17.00819 · Cited by 59 · see citing works
Pre-clinical Imaging of Invasive Candidiasis Using ImmunoPET/MR
Morad, H. O. J., Wild, A., Wiehr, S., Davies, G. E., Maurer, A., Pichler, B. J., & Thornton, C. R. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01996 · Cited by 59 · see citing works
Caplan, T., Polvi, E. J., Xie, J. L., Buckhalter, S., Leach, M. D., Robbins, N., & Cowen, L. E. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.04.084 · Cited by 56 · see citing works
Anderson, M. Z., Saha, A., Haseeb, A., & Bennett, R. J. (2017) · Microbiology · doi:10.1099/mic.0.000478 · Cited by 53 · see citing works
Kabir, M. A., Aḥmad, A., Greenberg, J. R., Wang, Y.-K., & Rustchenko, E. P. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0505625102 · Cited by 51 · see citing works
Genetic screen identifies adaptive aneuploidy as a key mediator of ER stress resistance in yeast
Beaupère, C., Dinatto, L., Wasko, B. M., Chen, R. B., VanValkenburg, L., Kiflezghi, M. G., Lee, M. B., Promislow, D., Dang, W., Kaeberlein, M. R., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1804264115 · Cited by 51 · see citing works
Levinson, T., Dahan, A., Novikov, A., Paran, Y., Berman, J., & Ben‐Ami, R. (2021) · Mycoses · doi:10.1111/myc.13191 · Cited by 50 · see citing works
Self-citationForche, A., Solis, N., Swidergall, M., Thomas, R. W., Guyer, A., Beach, A., Cromie, G., Le, G. T., Lowell, E., Pavelka, N., et al. (2019) · bioRxiv · doi:10.1371/journal.pgen.1008137 · Cited by 47 · see citing works
Self-citationModerate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci
Chang, Y. C., Lamichhane, A. K., Cai, H., Walter, P. J., Bennett, J. E., & Kwon-Chung, K. J. (2021) · Nature Communications · doi:10.1038/s41467-021-23745-1 · Cited by 45 · see citing works
The lipid-translocating exporter family and membrane phospholipid homeostasis in yeast
Manente, M., & Ghislain, M. (2009) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2009.00513.x · Cited by 44 · see citing works
Krysan, D. J. (2009) · Communicative & Integrative Biology · doi:10.4161/cib.2.3.8097 · Cited by 42 · see citing works
Genetic variation in aneuploidy prevalence and tolerance across Saccharomyces cerevisiae lineages
da Costa, E. S. F., Hose, J., Bensasson, D., & Gasch, A. P. (2021) · Genetics · doi:10.1093/genetics/iyab015 · Cited by 42 · see citing works
Aneuploidy and Gene Expression: Is there Dosage Compensation?
Kojima, S., & Cimini, D. (2019) · Epigenomics · doi:10.2217/epi-2019-0135 · Cited by 39 · see citing works
Bretthauer, R. K. (2009) · Current Drug Targets · doi:10.2174/138945009788488369 · Cited by 28 · see citing works
Trisomy of chromosome R confers resistance to triazoles in Candida albicans
Li, X., Yang, F., Li, D., Zhou, M., Wang, X., Xu, Q., Zhang, Y., Yan, L., & Jiang, Y. (2015) · Medical Mycology · doi:10.1093/mmy/myv002 · Cited by 28 · see citing works
Self-citationSuwunnakorn, S., Wakabayashi, H., & Rustchenko, E. (2016) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01888-16 · Cited by 28 · see citing works
Zhang, K., Zhang, L., Fang, Y.-H., Jin, X.-N., Qi, L., Wu, X., & Zheng, D. (2016) · FEMS Yeast Research · doi:10.1093/femsyr/fov118 · Cited by 27 · see citing works
Sah, S. K., Hayes, J. J., & Rustchenko, E. (2021) · PLOS Pathogens · doi:10.1371/journal.ppat.1009564 · Cited by 26 · see citing works
Thomas, E., Sircaik, S., Román, E., Brunel, J. M., Johri, A. K., Plá, J., & Panwar, S. L. (2015) · FEMS Yeast Research · doi:10.1093/femsyr/fov095 · Cited by 18 · see citing works
Kravets, A., Yang, F. J., Bethlendy, G., Cao, Y., Sherman, F., & Rustchenko, E. P. (2014) · FEMS Yeast Research · doi:10.1111/1567-1364.12155 · Cited by 15 · see citing works
Self-citationWidespread Occurrence of Dosage Compensation in Candida albicans
Kravets, A., Qin, H. H., Aḥmad, A., Bethlendy, G., Gao, Q., & Rustchenko, E. P. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010856 · Cited by 14 · see citing works
Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants
Tucker, C., Bhattacharya, S., Wakabayashi, H., Bellaousov, S., Kravets, A., Welle, S. L., Myers, J. R., Hayes, J. J., Bulger, M., & Rustchenko, E. P. (2018) · Scientific Reports · doi:10.1038/s41598-018-20106-9 · Cited by 14 · see citing works
Thomson, G. J., Kakade, P., Hirakawa, M. P., Ene, I. V., & Bennett, R. J. (2021) · G3 Genes Genomes Genetics · doi:10.1093/g3journal/jkab110 · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Molecular mechanisms governing antifungal drug resistance
Lee, Y., Robbins, N., & Cowen, L. E. (2023) · npj Antimicrobials and Resistance · doi:10.1038/s44259-023-00007-2 · Cited by 304 · see citing works
The importance of antimicrobial resistance in medical mycology
Gow, N. A. R., Johnson, C., Berman, J. G., Coste, A. T., Cuomo, C. A., Perlin, D. S., Bicanic, T. A., Harrison, T. S., Wiederhold, N. P., Bromley, M., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-32249-5 · Cited by 228 · see citing works
Self-citationIslam, T., Danishuddin, M., Tamanna, N. T., Matin, M. N., Barai, H. R., & Haque, M. A. (2024) · Plants · doi:10.3390/plants13192737 · Cited by 214 · see citing works
Natural proteome diversity links aneuploidy tolerance to protein turnover
Muenzner, J., Trébulle, P., Agostini, F., Zauber, H., Messner, C. B., Steger, M., Kilian, C., Lau, K., Barthel, N., Lehmann, A., et al. (2024) · Nature · doi:10.1038/s41586-024-07442-9 · Cited by 81 · see citing works
Self-citationYang, F., Scopel, E. F. C., Li, H., Sun, L.-L., Kawar, N., Cao, Y.-B., Jiang, Y.-Y., & Berman, J. (2023) · mBio · doi:10.1128/mbio.00227-23 · Cited by 62 · see citing works
Self-citationBergin, S. A., Zhao, F., Ryan, A. P., Müller, C. A., Nieduszynski, C. A., Zhai, B., Rolling, T., Hohl, T. M., Morio, F., Scully, J., et al. (2022) · mBio · doi:10.1128/mbio.01777-22 · Cited by 44 · see citing works
Beyond resistance: antifungal heteroresistance and antifungal tolerance in fungal pathogens
Yang, F., & Berman, J. (2024) · Current Opinion in Microbiology · doi:10.1016/j.mib.2024.102439 · Cited by 42 · see citing works
Self-citationFeng, Y., Lu, H., Whiteway, M., & Jiang, Y. (2023) · Journal of Global Antimicrobial Resistance · doi:10.1016/j.jgar.2023.10.019 · Cited by 40 · see citing works
Self-citationKukurudz, R. J., Chapel, M., Wonitowy, Q., Adamu Bukari, A.-R., Sidney, B., Sierhuis, R., & Gerstein, A. C. (2022) · G3 · doi:10.1093/g3journal/jkac156 · Cited by 35 · see citing works
A synthetic peptide mimic kills Candida albicans and synergistically prevents infection
Schaefer, S., Vij, R., Sprague, J. L., Austermeier, S., Dinh, H., Judzewitsch, P. R., Müller‐Loennies, S., Silva, T. L., Seemann, E. A., Qualmann, B., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-50491-x · Cited by 38 · see citing works
Götze, S., Vij, R., Burow, K., Thome, N. U., Urbat, L., Schlosser, N., Pflanze, S., Müller, R., Haensch, V. G., Schlabach, K., et al. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.2c11107 · Cited by 32 · see citing works
Aneuploidy Underlies Tolerance and Cross-Tolerance to Drugs in Candida parapsilosis
Yang, F. J., Lu, H., Wu, H., Fang, T., Berman, J. G., & Jiang, Y. (2021) · Microbiology Spectrum · doi:10.1128/spectrum.00508-21 · Cited by 27 · see citing works
Self-citationThe role of gene copy number variation in antimicrobial resistance in human fungal pathogens
Jay, A., Jordan, D., Gerstein, A. C., & Landry, C. R. (2025) · npj Antimicrobials and Resistance · doi:10.1038/s44259-024-00072-1 · Cited by 22 · see citing works
Aneuploidy enables cross-tolerance to unrelated antifungal drugs in Candida parapsilosis
Sun, L.-L., Li, H., Yan, T., Cao, Y., Jiang, Y., & Yang, F. J. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1137083 · Cited by 20 · see citing works
Self-citationAneuploidy Mediates Rapid Adaptation to a Subinhibitory Amount of Fluconazole in Candida albicans
Sun, L.-L., Li, H., Yan, T., Fang, T., Wu, H., Cao, Y., Lu, H., Jiang, Y., & Yang, F. J. (2023) · Microbiology Spectrum · doi:10.1128/spectrum.03016-22 · Cited by 17 · see citing works
Self-citationInsight into the Mechanisms and Clinical Relevance of Antifungal Heteroresistance
Su, Y., Li, Y., Yi, Q., Xu, Y., Sun, T., & Li, Y. (2025) · Journal of Fungi · doi:10.3390/jof11020143 · Cited by 18 · see citing works
“This tolerance is typically temperature-independent and frequently associated with aneuploidy, which may be lost upon temperature changes or ploidy recovery [114,115].”
Aneuploidy Underlies Brefeldin A‐Induced Antifungal Drug Resistance in Cryptococcus Neoformans
Zhang, Z.-H., Sun, L.-L., Fu, B.-Q., Deng, J., Jia, C.-L., Miao, M.-X., Yang, F., Cao, Y.-B., & Yan, T.-H. (2024) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2024.1397724 · Cited by 14 · see citing works
Self-citationCandida albicans’ inorganic phosphate transport and evolutionary adaptation to phosphate scarcity
Acosta-Zaldívar, M., Qi, W., Mishra, A., Roy, U., King, W. R., Li, Y., Patton-Vogt, J., Anderson, M. Z., & Köhler, J. R. (2024) · PLOS Genetics · doi:10.1371/journal.pgen.1011156 · Cited by 14 · see citing works
Amich, J., Bromley, M., Goldman, G. H., & Valero, C. (2025) · mBio · doi:10.1128/mbio.03475-24 · Cited by 14 · see citing works
Research advances and public health strategies in China on WHO priority fungal pathogens
Wang, Y., Han, L., Gong, J., Liu, L., Miao, B., & Xu, J. (2025) · Mycology: An International Journal on Fungal Biology · doi:10.1080/21501203.2025.2561612 · Cited by 13 · see citing works
Zheng, L., Xu, Y., Wang, C., & Guo, L. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1450557 · Cited by 12 · see citing works
David, H., Vasudevan, S., & Solomon, A. P. (2024) · Scientific Reports · doi:10.1038/s41598-024-62684-x · Cited by 12 · see citing works
Evolution and strain diversity advance exploration of Candida albicans biology
Anderson, M. Z., & Dietz, S. M. (2024) · mSphere · doi:10.1128/msphere.00641-23 · Cited by 11 · see citing works
Li, W., Whiteway, M., Hang, S., Yu, J., Lu, H., & Jiang, Y. (2025) · Nature Communications · doi:10.1038/s41467-025-62131-z · Cited by 13 · see citing works
“Elevated levels of misfolded proteins lead to ER stress, as evidenced by the increased susceptibility of the erg11 m / erg11 Δ mutant to tunicamycin, an inducer of ER stress 33 , compared to the ERG11 / erg11 Δ mutant (Supplementary Fig.”
Self-citationSah, S. K., Bhattacharya, S., Yadav, A., Husain, F., Ndiaye, A. B. K. T., Kruppa, M., Hayes, J. J., & Rustchenko, E. P. (2022) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00977-22 · Cited by 9 · see citing works
Rizzo, M., Soisangwan, N., Vega, S. B. P., Price, R. J., Uyl, C., Iracane, E., Shaw, M., Soetaert, J., Selmecki, A. M., & Buscaino, A. (2022) · PLoS Genetics · doi:10.1371/journal.pgen.1010576 · Cited by 8 · see citing works
Molecular mechanisms of neutrophil regulatory network in anti-Candida infection
Xu, Y., Cheng, R., Li, W., Yu, W., & Chen, C. (2025) · Frontiers in Immunology · doi:10.3389/fimmu.2025.1716645 · Cited by 8 · see citing works
Experimental evolution of drug resistance in human fungal pathogens
Gerstein, A. C., & Sethi, P. (2022) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2022.101965 · Cited by 9 · see citing works
Zheng, L., Xu, Y., Wang, C., Dong, Y., & Guo, L. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1470454 · Cited by 7 · see citing works
Li, H., Cao, Y., Yan, T., Jiang, Y., & Yang, F. J. (2022) · Journal of Infection · doi:10.1016/j.jinf.2022.09.028 · Cited by 7 · see citing works
Self-citationChromosome 1 trisomy confers resistance to aureobasidin A in Candida albicans
Zheng, L., Xu, Y., Dong, Y., Ma, X., Wang, C., Yang, F. J., & Guo, L. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1128160 · Cited by 7 · see citing works
Self-citationWang, H., Li, M., Wang, Q., Zhao, H., Jiang, M., Cui, Q., Lei, D., Jia, K., & Wang, F. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1648467 · Cited by 4 · see citing works
Serrano, R., González-Menéndez, V., Tormo, J. R., & Genilloud, O. (2023) · Journal of Fungi · doi:10.3390/jof9090883 · Cited by 6 · see citing works
Aneuploidy enables adaptation to brefeldin A in Candida albicans
Wang, W., Wang, C., Dong, Y., Yang, F. J., & Xu, Y. (2025) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2025.1562726 · Cited by 5 · see citing works
Self-citationBehind the scenes: Centromere-driven genomic innovations in fungal pathogens
Narayanan, A., Reza, M. H., & Sanyal, K. (2024) · PLoS Pathogens · doi:10.1371/journal.ppat.1012080 · Cited by 5 · see citing works
Zheng, L., Dong, Y., Wang, J., Jia, Y., Wang, W., Xu, Y., & Guo, L. (2025) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2025.1519323 · Cited by 6 · see citing works
Zheng, L., Xu, Y., Wang, C., Yang, F. J., Dong, Y., & Guo, L. (2023) · Microbiology Spectrum · doi:10.1128/spectrum.01790-23 · Cited by 3 · see citing works
Self-citationBergin, S. A., Zhao, F., Ryan, A. P., Müller, C. A., Nieduszynski, C. A., Zhai, B., Rolling, T., Hohl, T. M., Morio, F., Scully, J., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.12.16.473093 · Cited by 3 · see citing works
Zheng, L., Dong, Y., Wang, J., Zhang, M., Xu, Y., Ma, L., & Guo, L. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1528341 · Cited by 2 · see citing works
Zhang, M., Ma, W., Wang, J., Yang, F. J., Zhong, J., & Xu, Y. (2025) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2025.1664896 · Cited by 2 · see citing works
Self-citationFleres, G., Cheng, S., Badrane, H., Dupont, C. L., Espinoza, J. L., Abbey, D., Driscoll, E., Newbrough, A., Hao, B., Mansour, A., et al. (2025) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiaf495 · Cited by 2 · see citing works
Dong, Y., Ma, C., Wang, J., Bai, S., Wang, C., & Xu, Y. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1675175 · Cited by 2 · see citing works
Fleres, G., Cheng, S., Badrane, H., Dupont, C. L., Espinoza, J. L., Abbey, D., Driscoll, E., Newbrough, A., Hao, B., Mansour, A., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.16.618724 · Cited by 2 · see citing works
Negreira, G. H., Monsieurs, P., Dujardin, J.-C., & Domagalska, M. A. (2026) · eLife · doi:10.7554/elife.109350.1 · Cited by 2 · see citing works
Su, Y., Li, Y., Xiao, M., Guo, D., Perlin, D. S., Shor, E., Xu, Y., & Li, Y. (2026) · Advanced Science · doi:10.1002/advs.76369 · Cited by 2 · see citing works
Lipid-Based Drug Delivery Systems as Emerging Tools to Overcome Antifungal Resistance
Arana, L., Guridi, A., Sevillano, E., Tamayo, E. H., Eraso, E., Alkorta, I., & Maté, I. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27104487 · Cited by 2 · see citing works
Stack-Couture, S., Marsiglio-Librais, G. N., Lung, B., Genereaux, J. L., Halder, V., Dumeaux, V., Shapiro, R. S., & Lajoie, P. (2026) · Journal of Cell Science · doi:10.1242/jcs.264610 · Cited by 1 · see citing works
Negreira, G. H., Monsieurs, P., Dujardin, J., & Domagalska, M. A. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.10.675331 · Cited by 1 · see citing works
Diffusion-mediated quantification of dose-dependent antifungal drug tolerancePreprint
Koohi, S. R., Galon, C. M., & Charlebois, D. A. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.05.674536
Isolation of Candida albicans and Evaluation of Plant Extracts for Antifungal Activity.
El-Bashic, A. M., & Matter, H. A. (2025) · The Scientific Journal of University of Benghazi · doi:10.37376/sjuob.v38i2.7449
Negreira, G. H., Monsieurs, P., Dujardin, J.-C., & Domagalska, M. A. (2026) · eLife · doi:10.7554/elife.109350
Galon, C. M., & Charlebois, D. A. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.06.04.730223
Vellanki, S., DeMichaelis, N., Liao, C., Stajich, J., & Cramer, R. A. (2026) · mBio · doi:10.1128/mbio.00661-26
Antifungal resistance: from classical mechanisms to the expanding role of acetyltransferases
Werneck, S. M. C., Gouveia‐Eufrásio, L., de Freitas, G. J. C., de Carvalho, D. S., César, I. C., Rossato, L. L., Bastos, R. W., Peres, N. T. A., & Santos, D. A. (2026) · Journal of Biomedical Science · doi:10.1186/s12929-026-01273-8
A multiplex droplet digital PCR assay for chromosome copy-number determination in Candida albicans
Maciel, E. I., Ursuegui, S., Ahmed‐Seghir, S., Maufrais, C., Roy, S., Gautier, C., Brandt, P., Jovelet, C., Pitayu-Nugroho, L., & Ene, I. V. (2026) · G3 Genes Genomes Genetics · doi:10.1093/g3journal/jkag201
Endoplasmic reticulum stress reduces caspofungin resistance in Cryptococcus neoformans
Qu, X., Hu, G., Lee, C. W. J., Horianopoulos, L. C., & Kronstad, J. W. (2026) · mSphere · doi:10.1128/msphere.00451-26
Taş, E., Çetin, B., Yılmazer, A. N., & Işık, T. (2026) · Molecular Biology Reports · doi:10.1007/s11033-026-12737-3
Yang, F., Sun, L., Li, H., Fang, T., Guo, Q., Chen, N., & Jiang, Y. (2026) · Frontiers in Microbiology · doi:10.3389/fmicb.2026.1845986
Self-citationSaxena, A., Javed, M., & Trehanpati, N. (2026) · Journal of Gastroenterology · doi:10.1007/s00535-026-02524-z
Negreira, G. H., Monsieurs, P., Dujardin, J., & Domagalska, M. A. (2026) · eLife · doi:10.7554/elife.109350.2
- Authors
- Yang, F., Gritsenko, V., Slor Futterman, Y., Gao, L., Zhen, C., Lu, H., Jiang, Y.-Y., & Berman, J.
- Affiliations
- Tongji University (CN)
- Tel Aviv University (IL)
- Shmunis School of Biomedical and Cancer Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
- Department of Pharmacy, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China
- Corresponding author
- Berman, Judith
- Venue
- mBio
- Publisher
- American Society for Microbiology
- Published
- 2021-08-31
- Volume
- 12
- Issue
- 4
- Pages
- e0227221-e0227221
- ISSN
- 2150-7511 (ISSN-L)
- PMC
- PMC8406271
- OpenAlex
- W3196781484
- Cited by
- 59
- References
- 74
- Citations by year
- 2026: 13 · 2025: 16 · 2024: 12 · 2023: 9 · 2022: 7 · 2021: 2
- FWCI
- 2.38
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- mBio (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Keywords
- tunicamycin, Candida albicans, antifungal drug tolerance, aneuploidy, antifungal resistance, drug tolerance, fungal pathogens
- MeSH terms
- Aneuploidy, Antifungal Agents, Candida albicans, Stress, Physiological, Tunicamycin, Drug Resistance, Fungal
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
