Cited by 110Autophagy in Disease and TherapyGenetics, Aging, and Longevity in Model OrganismsUbiquitin and proteasome pathways

Age-related changes in the regulation of autophagic proteolysis in rat isolated hepatocytes

Donati, A., Cavallini, G., Paradiso, C., Vittorini, S., Pollera, M., Gori, Z., Bergamini, E.·2001·The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

Abstract

During intervals between meals, autophagy is a major source of nutrients and may remove damaged organelles and membranes. Age-related changes in the regulation of autophagic proteolysis were studied by monitoring the rate of valine release from liver cells of 2-, 6-, 12-, 18-, and 24-month-old male Sprague-Dawley rats fed ad libitum, and incubated in vitro with added amino acids and 10(-7) M of insulin or glucagon. The maximum rate of proteolysis and its maximum inhibition by amino acids were reached at 6 months and declined thereafter. In contrast, the rate of protein degradation in the presence of high concentrations of amino acids was not affected by aging. The inhibitor effect of insulin was additive to that of amino acids and was not altered significantly by age. The conclusion is that altered regulation of autophagic proteolysis decreases susceptibility of older cells to lysosomal degradation, and it may lead to the accumulation of altered organelles and membranes.

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Crossref · 10.1093/gerona/56.7.b288

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  • The lysosomal-autophagy system declines in a variety of tissues with age.

    “Although there is conflicting evidence regarding the effect of aging on various proteolytic pathways (for reviews, see Attaix et al., 2005; Combaret et al., 2009), it has been reported that the lysosomal-autophagy system declines in a variety of tissues with age (Cuervo and Dice, 2000; Donati et al., 2001; Wohlgemuth et al., 2010).”

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Authors
Donati, A., Cavallini, G., Paradiso, C., Vittorini, S., Pollera, M., Gori, Z., & Bergamini, E.
Affiliations
University of Pisa (IT)
Corresponding author
Donati, A.
Venue
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Published
2001-07-01
Volume
56
Issue
7
Pages
B288-B293
ISSN
  • 1079-5006 (ISSN-L)
  • 1758-535X
OpenAlex
W2005624025
Cited by
110
References
41
Citations by year
2025: 3 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 12 · 2017: 6 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 5 · 2012: 9
FWCI
2.29
Type
journal article
Language
en
Open access
closed
Locations
  • The Journals of Gerontology Series A (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministero dell'Università e della Ricerca
Topics
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Keywords
autophagy, aging, hepatocytes, lysosomal degradation, insulin, glucagon, amino acids, protein degradation, age-related decline
MeSH terms
Aging, Amino Acids, Analysis of Variance, Animals, Autophagy, Glucagon, Insulin, Liver, Male, Microscopy, Electron, Valine, Rats, Sprague-Dawley, Hepatocytes, Rats, In Vitro Techniques

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