The effect of age on formation and elimination of autophagic vacuoles in mouse hepatocytes
Abstract
To gain an understanding of the mechanisms of lipofuscin (LF) accumulation in aging cells we studied both the formation and the elimination of autophagic vacuoles (AV) in hepatocytes of adult (5–6 months) and old (20–21 months) CBA male mice. For the evaluation of AV formation we determined the degree of AV accumulation 4 h after the injection of vinblastine, which blocks the fusion of AV with lysosomes. Other animals were treated with Triton X-100, which provokes the appearance of AV, and then, after 4 h, with cycloheximide to block the appearance of newly formed AV. AV elimination was then evaluated during the next 30 minutes. In our quantitative electron microscopic study of the liver tissue of these mice, we found a decrease in AV formation rate as well as a decrease in the intensity of AV elimination in hepatocytes of old versus young adult animals. The results indicate that the slowing down of AV elimination with age may play an important role in LF accumulation. Further, LF accumulation manifests itself despite decreased autophagy in aging.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In old rodent livers, cargo sequestration by double membrane vesicles still takes place, but elimination of these vesicles by fusion with the lysosomal compartment is severely impaired.
“although cargo sequestration by the double membrane vesicles still takes place in the old tissues, the elimination of these vesicles by fusion with the lysosomal compartment was severely impaired (Fig. 3) (Terman, 1995)”
From Protein Homeostasis and Aging: the importance of exquisite quality control (2011) · cites this paper as Terman, 1995
Cited by
Sorted by citations
Selective degradation of mitochondria by mitophagy
Kim, I., Rodriguez-Enriquez, S., & Lemasters, J. J. (2007) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2007.03.034 · Cited by 1,558 · see citing works
Kregel, K. C., & Zhang, H. J. (2007) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00327.2006 · Cited by 883 · see citing works
Autophagy and aging: The importance of maintaining "clean" cells
Cuervo, A. M., Bergamini, E., Brunk, U. T., Dröge, W., Ffrench, M., & Terman, A. (2005) · Autophagy · doi:10.4161/auto.1.3.2017 · Cited by 792 · see citing works
Self-citationAutophagy as a promoter of longevity: insights from model organisms
Hansen, M., Rubinsztein, D. C., & Walker, D. W. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0033-y · Cited by 754 · see citing works
Aging as an event of proteostasis collapse
Taylor, R. C., & Dillin, A. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a004440 · Cited by 538 · see citing works
New insights into the role of mitochondria in aging: mitochondrial dynamics and more
Seo, A. Y., Joseph, A.-M., Dutta, D., Hwang, J. C. Y., Aris, J. P., & Leeuwenburgh, C. (2010) · Journal of Cell Science · doi:10.1242/jcs.070490 · Cited by 522 · see citing works
Terman, A., Kurz, T., Navratil, M., Arriaga, E. A., & Brunk, U. T. (2010) · Antioxidants & Redox Signaling · doi:10.1089/ars.2009.2598 · Cited by 490 · see citing works
Self-citationProtein Homeostasis and Aging: the importance of exquisite quality control
Koga, H., Kaushik, S., & Cuervo, A. M. (2011) · Ageing research reviews · doi:10.1016/j.arr.2010.02.001 · Cited by 476 · see citing works
Inhibition of autophagy in the heart induces age-related cardiomyopathy
Taneike, M., Yamaguchi, O., Nakai, A., Hikoso, S., Takeda, T., Mizote, I., Oka, T., Tamai, T., Oyabu, J., Murakawa, T., et al. (2010) · Autophagy · doi:10.4161/auto.6.5.11947 · Cited by 450 · see citing works
Wohlgemuth, S. E., Seo, A. Y., Marzetti, E., Lees, H. A., & Leeuwenburgh, C. (2010) · Experimental Gerontology · doi:10.1016/j.exger.2009.11.002 · Cited by 397 · see citing works
Lipofuscin: Mechanisms of formation and increase with age
TERMAN, A., & BRUNK, U. T. (1998) · APMIS · doi:10.1111/j.1699-0463.1998.tb01346.x · Cited by 387 · see citing works
Self-citationLysosomes in iron metabolism, ageing and apoptosis
Kurz, T., Terman, A., Gustafsson, B., & Brunk, U. T. (2008) · Histochemistry and Cell Biology · doi:10.1007/s00418-008-0394-y · Cited by 302 · see citing works
Self-citationLysosomes and oxidative stress in aging and apoptosis
Kurz, T., Terman, A., Gustafsson, B., & Brunk, U. T. (2008) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2008.01.009 · Cited by 287 · see citing works
Self-citationAutophagy and the hallmarks of aging
Kaushik, S., Tasset, I., Arias, E., Pampliega, O., Wong, E., Martinez-Vicente, M., & Cuervo, A. M. (2021) · Ageing Research Reviews · doi:10.1016/j.arr.2021.101468 · Cited by 285 · see citing works
Lysosomal cell death mechanisms in aging
Gómez-Sintes, R., Ledesma, M. D., & Boya, P. (2016) · Ageing Research Reviews · doi:10.1016/j.arr.2016.02.009 · Cited by 216 · see citing works
Altered dynamics of the lysosomal receptor for chaperone-mediated autophagy with age
Kiffin, R., Kaushik, S., Zeng, M., Bandyopadhyay, U., Zhang, C., Massey, A. C., Martinez-Vicente, M., & Cuervo, A. M. (2007) · Journal of Cell Science · doi:10.1242/jcs.001073 · Cited by 213 · see citing works
Luo, L., Lu, A.-M., Wang, Y., Hong, A., Chen, Y., Hu, J., Li, X., & Qin, Z.-H. (2013) · Experimental Gerontology · doi:10.1016/j.exger.2013.02.009 · Cited by 192 · see citing works
Salminen, A., Kaarniranta, K., & Kauppinen, A. (2013) · Ageing Research Reviews · doi:10.1016/j.arr.2012.11.004 · Cited by 173 · see citing works
The Role of Tau Oligomers in the Onset of Alzheimer's Disease Neuropathology
Cárdenas-Aguayo, M. D. C., Gómez-Virgilio, L., DeRosa, S., & Meraz-Ríos, M. A. (2014) · ACS Chemical Neuroscience · doi:10.1021/cn500148z · Cited by 120 · see citing works
Age-related changes in the regulation of autophagic proteolysis in rat isolated hepatocytes
Donati, A., Cavallini, G., Paradiso, C., Vittorini, S., Pollera, M., Gori, Z., & Bergamini, E. (2001) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/56.7.b288 · Cited by 110 · see citing works
Mitochondrial function and mitochondrial DNA maintenance with advancing age
Gaziev, A. I., Abdullaev, S., & Podlutsky, A. (2014) · Biogerontology · doi:10.1007/s10522-014-9515-2 · Cited by 77 · see citing works
Transcriptomic profiles of aging in purified human immune cells
Reynolds, L. M., Ding, J., Taylor, J. R., Lohman, K., Soranzo, N., de la Fuente, A., Liu, T. F., Johnson, C., Barr, R. G., Register, T. C., et al. (2015) · BMC Genomics · doi:10.1186/s12864-015-1522-4 · Cited by 62 · see citing works
Maximizing Longevity and Healthspan: Multiple Approaches All Converging on Autophagy
Bareja, A., Lee, D. E., & White, J. P. (2019) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2019.00183 · Cited by 41 · see citing works
Coen, P. M., Huo, Z., Tranah, G. J., Barnes, H. N., Zhang, X., Wolff, C. A., Wu, K., Cawthon, P. M., Hepple, R. T., Toledo, F. G. S., et al. (2024) · Aging Cell · doi:10.1111/acel.14118 · Cited by 17 · see citing works
“In fact, poor clearance of already formed autophagosomes with age was one of the first defects iden - tified in autophagy in model systems (Terman, 1995 ).”
BackgroundWu, Q., E, S., Yamamoto, K., & Tsuduki, T. (2019) · Biogerontology · doi:10.1007/s10522-018-9777-1 · Cited by 11 · see citing works
Retracted
- Authors
- Terman, A.
- Affiliations
- D. F. Chebotarev State Institute of Gerontology of the National Academy of Medical Sciences of Ukraine (UA)
- Corresponding author
- Terman, A.
- Venue
- Gerontology
- Publisher
- S. Karger AG
- Published
- 2009-04-15
- Volume
- 41 Suppl 2
- Pages
- 319-326
- ISSN
- 0304-324X (ISSN-L)
- 1423-0003
- OpenAlex
- W2053394933
- Cited by
- 162
- References
- 0
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 1 · 2022: 3 · 2021: 9 · 2020: 4 · 2019: 9 · 2018: 10 · 2017: 7 · 2016: 5 · 2015: 4 · 2014: 5 · 2013: 7 · 2012: 10
- FWCI
- 8.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gerontology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- autophagosomes, lipofuscin accumulation, aging, hepatocytes, autophagy, vinblastine, Triton X-100, cycloheximide, lysosome fusion, age-related decline
- MeSH terms
- Aging, Antineoplastic Agents, Phytogenic, Autophagy, Cells, Cultured, Lipofuscin, Lysosomes, Microscopy, Electron, Vacuoles, Vinblastine
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
