Housing Density Does Not Influence the Longevity Effect of Calorie Restriction
Abstract
This study examined the effect of housing density on the longevity-extending and disease-delaying actions of calorie restriction (CR). Singly or multiply housed (four per cage) mice were either fed ad libitum (AL) or were on CR beginning at 2 months. All CR mice were fed 40% less food than were multiply housed AL mice. CR increased median longevity by 19%, and housing density had no effect on this increase. CR also reduced neoplastic lesions in both housing groups, but lymphoma, the most common neoplasm, was reduced more in singly than in multiply housed mice. Singly housed AL mice ate 40% more food than did multiply housed AL mice, but weighed the same and lived as long as multiply housed AL mice. These results indicate that CR can extend life span as effectively in multiply as in singly housed mice, even though housing density can differentially affect the cancer-reducing effect of CR.
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Median lifespan of vehicle-treated C57BL/6 males, but not females, was short of the normal range of lifespans for unmanipulated males of this strain at different laboratories.
“We note that the median lifespan of vehicle-treated C57BL/6 males, but not females, was short of the normal range of lifespans for unmanipulated males of this strain at different laboratories (Extended Data Fig. 4c, d) 15 - 28 , suggesting that repetitive vehicle injection stress may have negatively impacted C57BL/6 male longevity (Supplemenary Text).”
From Naturally occurring p16Ink4a-positive cells shorten healthy lifespan (2016) · cites this paper as [21]
Animal Husbandry (p. 2) identifies the study animals as male C57BL/6J mice, and Effect of Food Intake & Housing Density on Life Span (p. 4) reports a median survival of approximately 930 days for its ad-libitum groups. This provides a male lifespan benchmark for the citing paper’s comparison, but this source does not assess vehicle-treated mice, females, or whether either sex falls outside a normal range across laboratories. (LLM, conf=0.84)
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- Authors
- Ikeno, Y., Hubbard, G. B., Lee, S., Richardson, A., Strong, R., Diaz, V., & Nelson, J. F.
- Affiliations
- South Texas Veterans Health Care System (US)
- Texas Biomedical Research Institute (US)
- The University of Texas at San Antonio Health Science Center (US)
- Corresponding author
- Ikeno, Y.
- Venue
- The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-12-01
- Volume
- 60
- Issue
- 12
- Pages
- 1510-1517
- ISSN
- 1079-5006 (ISSN-L)
- 1758-535X
- OpenAlex
- W2114331328
- Cited by
- 83
- References
- 41
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2018: 8 · 2017: 2 · 2016: 2 · 2015: 7 · 2014: 2 · 2013: 7 · 2012: 9
- FWCI
- 0.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journals of Gerontology Series A (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (AG 19899, 1P30- AG13319, R01AG019899, P30AG013319)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Physiology › Dietary Effects on Health
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Keywords
- caloric restriction, housing density, longevity, lifespan extension, neoplastic lesions, lymphoma, mice, ad libitum feeding, cancer prevention
- MeSH terms
- Animals, Crowding, Longevity, Male, Mice, Inbred C57BL, Caloric Restriction, Mice
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