Interplay between the temporal dynamics of the vaginal microbiota and human papillomavirus detection
Open accessAbstract
BACKGROUND: We sought to describe the temporal relationship between vaginal microbiota and human papillomavirus (HPV) detection. METHODS: Thirty-two reproductive-age women self-collected midvaginal swabs twice weekly for 16 weeks (937 samples). Vaginal bacterial communities were characterized by pyrosequencing of barcoded 16S rRNA genes and clustered into 6 community state types (CSTs). Each swab was tested for 37 HPV types. The effects of CSTs on the rate of transition between HPV-negative and HPV-positive states were assessed using continuous-time Markov models. RESULTS: Participants had an average of 29 samples, with HPV point prevalence between 58%-77%. CST was associated with changes in HPV status (P<.001). Lactobacillus gasseri-dominated CSTs had the fastest HPV remission rate, and a low Lactobacillus community with high proportions of the genera Atopobium (CST IV-B) had the slowest rate compared to L. crispatus-dominated CSTs (adjusted transition rate ratio [aTRR], 4.43, 95% confidence interval [CI], 1.11-17.7; aTRR, 0.33, 95% CI, .12-1.19, respectively). The rate ratio of incident HPV for low Lactobacillus CST IV-A was 1.86 (95% CI, .52-6.74). CONCLUSIONS: Vaginal microbiota dominated by L. gasseri was associated with increased clearance of detectable HPV. Frequent longitudinal sampling is necessary for evaluation of the association between HPV detection and dynamic microbiota.
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CSTs III and IV are frequently linked with presence of HPV infection and development of preinvasive cervical disease states.
“Recent observational cross-sectional studies support the concept that CSTs III and IV, in particular, are frequently linked with the presence of HPV infection and development of preinvasive cervical disease states (Lee J.E. et al., 2013; Brotman et al., 2014; Mitra et al., 2015).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Brotman et al., 2014
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Viral Infections, the Microbiome, and Probiotics
Harper, A., Vijayakumar, V., Ouwehand, A. C., ter Haar, J., Obis, D., Espadaler, J., Binda, S., Desiraju, S., & Day, R. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.596166 · Cited by 177 · see citing works
- Authors
- Brotman, R. M., Shardell, M. D., Gajer, P., Tracy, J. K., Zenilman, J. M., Ravel, J., & Gravitt, P. E.
- Affiliations
- University of Maryland, Baltimore (US)
- Johns Hopkins University (US)
- Corresponding author
- Brotman, R. M.
- Venue
- Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-06-18
- Volume
- 210
- Issue
- 11
- Pages
- 1723-1733
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- PMC
- PMC4296189
- OpenAlex
- W2152821355
- Cited by
- 411
- References
- 51
- Citations by year
- 2026: 33 · 2025: 39 · 2024: 35 · 2023: 50 · 2022: 56 · 2021: 55 · 2020: 40 · 2019: 35 · 2018: 18 · 2017: 21 · 2016: 16 · 2015: 11 · 2014: 1 · 2013: 1
- FWCI
- 13.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (K25AG034216)
- National Cancer Institute (R01CA123467)
- National Institute of Allergy and Infectious Diseases (R03AI061131, UH2AI083264, K01AI080974)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- vaginal microbiota, human papillomavirus, HPV clearance, Lactobacillus gasseri, Lactobacillus crispatus, community state types, Atopobium, HPV incidence, temporal dynamics, continuous-time Markov model
- MeSH terms
- Adolescent, Adult, Female, Humans, Markov Chains, Middle Aged, Risk Factors, Vagina, Cluster Analysis, Vaginosis, Bacterial, Papillomavirus Infections, Alphapapillomavirus, Metagenome, Young Adult, Microbiota
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