Identification of key bacteria involved in the induction of incident bacterial vaginosis: a prospective study.
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Background: The sequence of events preceding incident bacterial vaginosis (iBV) is unclear. Methods: African American women who have sex with women, who had no Amsel criteria and Nugent scores of 0-3, were followed for 90 days to detect iBV (defined as a Nugent score of 7-10 on at least 2-3 consecutive days), using self-collected vaginal swab specimens. For women with iBV (cases) and women maintaining normal vaginal flora (healthy women), 16S ribosomal RNA gene sequencing targeting V4 was performed. Longitudinal vaginal microbiome data were analyzed. Results: Of 204 women screened, 42 enrolled; of these, 45% developed iBV. Sequencing was performed on 448 specimens from 14 cases and 8 healthy women. Among healthy women, Lactobacillus crispatus dominated the vaginal microbiota in 75%. In contrast, prior to iBV, the vaginal microbiota in 79% of cases was dominated by Lactobacillus iners and/or Lactobacillus jensenii/Lactobacillus gasseri. The mean relative abundance of Prevotella bivia, Gardnerella vaginalis, Atopobium vaginae, and Megasphaera type I became significantly higher in cases 4 days before (P. bivia), 3 days before (G. vaginalis), and on the day of (A. vaginae and Megasphaera type I) iBV onset. The mean relative abundance of Sneathia sanguinegens, Finegoldia magna, BV-associated bacteria 1-3, and L. iners was not significantly different between groups before onset of iBV. Conclusion: G. vaginalis, P. bivia, A. vaginae, and Megasphaera type I may play significant roles in iBV.
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These studies have continued to support, although have not proven, the hypothesis that sexual behaviors, specific BV-associated bacteria, and possibly exchange of vaginal or extravaginal microbiota between partners might be involved in the pathogenesis of BV among WSW.
“These studies have continued to support, although have not proven, the hypothesis that sexual behaviors, specific BV-associated bacteria, and possibly exchange of vaginal or extravaginal microbiota (e.g., oral bacterial communities) between partners might be involved in the pathogenesis of BV among WSW.”
From Sexually Transmitted Infections Treatment Guidelines, 2021 (2021) · cites this paper as [323]
The paper’s introduction says BV epidemiology strongly indicates sexual transmission, and its Discussion reports that sexual activity coincided with changes in vaginal bacterial communities. Its conclusions also suggest that specific bacteria may help induce incident BV in women who have sex with women. It does not examine microbiota exchange between partners, including oral communities, so that part of the hypothesis remains unsupported by this paper (section labels provided; pages unavailable). (LLM, conf=0.86)
Experimental — accuracy not yet measuredA BV pathogenesis study in WSW reported that Prevotella bivia, Gardnerella vaginalis, and Atopobium vaginae might have substantial roles in development of incident BV.
“A BV pathogenesis study in WSW reported that Prevotella bivia, Gardnerella vaginalis, and Atopobium vaginae might have substantial roles in development of incident BV (323).”
From Sexually Transmitted Infections Treatment Guidelines, 2021 (2021) · cites this paper as [323]
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Self-citation
- Authors
- Muzny, C. A., Blanchard, E., Taylor, C. M., Aaron, K. J., Talluri, R., Griswold, M. E., Redden, D. T., Luo, M., Welsh, D. A., Van Der Pol, W. J., Lefkowitz, E. J., Martin, D. H., & Schwebke, J. R.
- Affiliations
- Division of Infectious Diseases, New Orleans, Louisiana
- Department of Microbiology, Immunology, and Parasitology, New Orleans, Louisiana
- University of Mississippi Medical Center (US)
- Department of Biostatistics, School of Public Health, New Orleans, Louisiana
- Microbial Genomics Resource Group, School of Medicine, New Orleans, Louisiana
- Center for Clinical and Translational Science, New Orleans, Louisiana
- University of Alabama at Birmingham (US)
- Tulane University (US)
- Corresponding author
- Muzny, Christina A
- ORCID
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2018-05-25
- Volume
- 218
- Issue
- 6
- Pages
- 966-978
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- PMC
- PMC6093354
- OpenAlex
- W2801244393
- Cited by
- 145
- References
- 50
- Citations by year
- 2026: 10 · 2025: 15 · 2024: 25 · 2023: 25 · 2022: 16 · 2021: 23 · 2020: 16 · 2019: 13 · 2018: 2
- FWCI
- 10.90
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (K23AI106957)
- National Institute of General Medical Sciences (P20GM103424)
- Louisiana Biomedical Research Network (UL1TR001417)
- University of Alabama-Birmingham Center for Clinical and Translational Science
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- bacterial vaginosis, vaginal microbiota, Gardnerella vaginalis, Prevotella bivia, Atopobium vaginae, Megasphaera, Lactobacillus iners, Lactobacillus crispatus, Lactobacillus jensenii, Lactobacillus gasseri, African American women, women who have sex with women, vaginal dysbiosis, disease onset
- MeSH terms
- Adult, Black or African American, DNA, Bacterial, DNA, Ribosomal, Female, Gardnerella vaginalis, Humans, Longitudinal Studies, Prospective Studies, RNA, Ribosomal, 16S, Vagina, Vaginosis, Bacterial, Sequence Analysis, DNA, Prevotella, Megasphaera, Young Adult, Microbiota
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