The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Non-viral methods lack the potential to deliver CRISPR/Cas9 in clinical settings in the near future due to difficulties in yielding proper ratio for gene editing and lack of transduction efficacy.
“However, due to difficulties in yielding proper ratio for gene editing and the lack of transduction efficacy, these methods lack the potential to deliver CRISPR/Cas9 in clinical settings in the near future [65].”
From Viral Delivery Systems for CRISPR (2019) · cites this paper as [65]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Genome-scale CRISPR-Cas9 knockout screening in human cells
Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
CRISPR-Cas systems for editing, regulating and targeting genomes
Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2842 · Cited by 3,153
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937
Crystal structure of Cas9 in complex with guide RNA and target DNA
Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306
Mali, P., Aach, J., Stranges, P. B., Esvelt, K. M., Moosburner, M., Kosuri, S., Yang, L., & Church, G. M. (2013) · Nature Biotechnology · doi:10.1038/nbt.2675 · Cited by 1,752
Koike-Yusa, H., Li, Y., Tan, E.-P., Velasco-Herrera, M. D. C., & Yusa, K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2800 · Cited by 1,064
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
Perez, E. E., Wang, J., Miller, J. C., Jouvenot, Y., Kim, K. A., Liu, O., Wang, N., Lee, G., Bartsevich, V. V., Lee, Y.-L., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1410 · Cited by 999
Heckl, D., Kowalczyk, M. S., Yudovich, D., Belizaire, R., Puram, R. V., McConkey, M. E., Thielke, A., Aster, J. C., Regev, A., & Ebert, B. L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2951 · Cited by 555
In vivo genome editing restores haemostasis in a mouse model of haemophilia
Li, H., Haurigot, V., Doyon, Y., Li, T., Wong, S. Y., Bhagwat, A. S., Malani, N., Anguela, X. M., Sharma, R., Ivanciu, L., et al. (2011) · Nature · doi:10.1038/nature10177 · Cited by 548
Flagged first, then by citations
Research works printed but not linked: 47
- Gaj, 2013, ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering, Trends Biotechnol., 31, 397, 10.1016/j.tibtech.2013.04.004 · DOI · Paper page
- Perez-Pinera, 2012, Advances in targeted genome editing, Curr. Opin. Chem. Biol., 16, 268, 10.1016/j.cbpa.2012.06.007 · DOI
- Gersbach, 2014, Synthetic zinc finger proteins: the advent of targeted gene regulation and genome modification technologies, Acc. Chem. Res., 10.1021/ar500039w · DOI
- Urnov, 2005, Highly efficient endogenous human gene correction using designed zinc-finger nucleases, Nature, 435, 646, 10.1038/nature03556 · DOI
- Zou, 2011, Site-specific gene correction of a point mutation in human iPS cells derived from an adult patient with sickle cell disease, Blood, 118, 4599, 10.1182/blood-2011-02-335554 · DOI
- Sebastiano, 2011, In situ genetic correction of the sickle cell anemia mutation in human induced pluripotent stem cells using engineered zinc finger nucleases, Stem Cells, 29, 1717, 10.1002/stem.718 · DOI
- Osborn, 2013, TALEN-based gene correction for epidermolysis bullosa, Mol. Ther., 21, 1151, 10.1038/mt.2013.56 · DOI · Paper page
- Ousterout, 2013, Reading frame correction by targeted genome editing restores dystrophin expression in cells from Duchenne muscular dystrophy patients, Mol. Ther., 21, 1718, 10.1038/mt.2013.111 · DOI
- Tebas, 2014, Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV, N. Engl. J. Med., 370, 901, 10.1056/NEJMoa1300662 · DOI · Paper page
- Zhang, 2000, Synthetic zinc finger transcription factor action at an endogenous chromosomal site. Activation of the human erythropoietin gene, J. Biol. Chem., 275, 33850, 10.1074/jbc.M005341200 · DOI
- Liu, 2001, Regulation of an endogenous locus using a panel of designed zinc finger proteins targeted to accessible chromatin regions. Activation of vascular endothelial growth factor A, J. Biol. Chem., 276, 11323, 10.1074/jbc.M011172200 · DOI
- Maeder, 2013, CRISPR RNA-guided activation of endogenous human genes, Nat. Methods, 10, 977, 10.1038/nmeth.2598 · DOI · Paper page
- Maeder, 2013, Robust, synergistic regulation of human gene expression using TALE activators, Nat. Methods, 10, 243, 10.1038/nmeth.2366 · DOI
- Perez-Pinera, 2013, Synergistic and tunable human gene activation by combinations of synthetic transcription factors, Nat. Methods, 10, 239, 10.1038/nmeth.2361 · DOI
- Perez-Pinera, 2013, RNA-guided gene activation by CRISPR-Cas9-based transcription factors, Nat. Methods, 10, 973, 10.1038/nmeth.2600 · DOI · Paper page
- Gersbach, 2014, Activating human genes with zinc finger proteins, transcription activator-like effectors and CRISPR/Cas9 for gene therapy and regenerative medicine, Expert Opin. Ther. Targets, 18, 835, 10.1517/14728222.2014.913572 · DOI
- Kabadi, 2014, Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression, Methods, 10.1016/j.ymeth.2014.06.014 · DOI
- Maeder, 2013, Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins, Nat. Biotechnol., 31, 1137, 10.1038/nbt.2726 · DOI
- Konermann, 2013, Optical control of mammalian endogenous transcription and epigenetic states, Nature, 500, 472, 10.1038/nature12466 · DOI · Paper page
- Siddique, 2013, Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity, J. Mol. Biol., 425, 479, 10.1016/j.jmb.2012.11.038 · DOI
- Mendenhall, 2013, Locus-specific editing of histone modifications at endogenous enhancers, Nat. Biotechnol., 31, 1133, 10.1038/nbt.2701 · DOI
- Jinek, 2013, RNA-programmed genome editing in human cells, eLife, 2, e00471, 10.7554/eLife.00471 · DOI · Paper page
- Cho, 2013, Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease, Nat. Biotechnol., 31, 230, 10.1038/nbt.2507 · DOI · Paper page
- Cheng, 2013, Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system, Cell Res., 23, 1163, 10.1038/cr.2013.122 · DOI
- Farzadfard, 2013, Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas, ACS Synthet. Biol., 2, 604, 10.1021/sb400081r · DOI
- Kearns, 2014, Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells, Development, 141, 219, 10.1242/dev.103341 · DOI
- Ohshima, 1981, Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA, Nucleic Acids Res., 9, 5145, 10.1093/nar/9.19.5145 · DOI
- Myslinski, 2001, An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene, Nucleic Acids Res., 29, 2502, 10.1093/nar/29.12.2502 · DOI
- Murphy, 1987, The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter, Cell, 51, 81, 10.1016/0092-8674(87)90012-2 · DOI
- Salmon, 2006, Production and titration of lentiviral vectors, Curr. Protoc. Neurosci., 4, 4.21.1, 10.1002/0471142301.ns0421s37 · DOI
- Guschin, 2010, A rapid and general assay for monitoring endogenous gene modification, Methods Mol. Biol., 649, 247, 10.1007/978-1-60761-753-2_15 · DOI
- Perez-Pinera, 2012, Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases, Nucleic Acids Res., 40, 3741, 10.1093/nar/gkr1214 · DOI
- Duda, 2014, High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs, Nucleic Acids Res., 42, e84, 10.1093/nar/gku251 · DOI
- Gou, 2007, A novel approach for the construction of multiple shRNA expression vectors, J. Gene Med., 9, 751, 10.1002/jgm.1080 · DOI
- Hockemeyer, 2009, Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases, Nat. Biotechnol., 27, 851, 10.1038/nbt.1562 · DOI
- DeKelver, 2010, Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome, Genome Res., 20, 1133, 10.1101/gr.106773.110 · DOI
- Wang, 2014, Genetic screens in human cells using the CRISPR-Cas9 system, Science, 343, 80, 10.1126/science.1246981 · DOI · Paper page
- Esvelt, 2013, Orthogonal Cas9 proteins for RNA-guided gene regulation and editing, Nat. Methods, 10, 1116, 10.1038/nmeth.2681 · DOI · Paper page
- Fonfara, 2013, Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems, Nucleic Acids Res., 42, 2577, 10.1093/nar/gkt1074 · DOI · Paper page
- Beerli, 2000, Chemically regulated zinc finger transcription factors, J. Biol. Chem., 275, 32617, 10.1074/jbc.M005108200 · DOI
- Mercer, 2013, Regulation of endogenous human gene expression by ligand-inducible TALE transcription factors, ACS Synthet. Biol.
- Polstein, 2012, Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors, J. Am. Chem. Soc., 134, 16480, 10.1021/ja3065667 · DOI
- Lombardo, 2007, Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery, Nat. Biotechnol., 25, 1298, 10.1038/nbt1353 · DOI · Paper page
- Tsai, 2014, Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing, Nat. Biotechnol., 32, 569, 10.1038/nbt.2908 · DOI · Paper page
- Nissim, 2014, Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells, Mol. Cell., 54, 698, 10.1016/j.molcel.2014.04.022 · DOI
- Gao, 2014, Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing, J. Integr. Plant Biol., 56, 343, 10.1111/jipb.12152 · DOI
- Persons, 2010, Lentiviral vector gene therapy: effective and safe, Mol. Ther., 18, 861, 10.1038/mt.2010.70 · DOI
Cited by
Sorted by citations
Adamson, B., Norman, T. M., Jost, M., Cho, M. Y., Nuñez, J. K., Chen, Y., Villalta, J. E., Gilbert, L. A., Horlbeck, M. A., Hein, M. Y., et al. (2016) · Cell · doi:10.1016/j.cell.2016.11.048 · Cited by 1,193
Delivering CRISPR: a review of the challenges and approaches
Lino, C. A., Harper, J. C., Carney, J. P., & Timlin, J. A. (2018) · Drug Delivery · doi:10.1080/10717544.2018.1474964 · Cited by 1,185
Thakore, P. I., D'Ippolito, A. M., Song, L., Safi, A., Shivakumar, N. K., Kabadi, A. M., Reddy, T. E., Crawford, G. E., & Gersbach, C. A. (2015) · Nature Methods · doi:10.1038/nmeth.3630 · Cited by 1,012
Horlbeck, M. A., Gilbert, L. A., Villalta, J. E., Adamson, B., Pak, R. A., Chen, Y., Fields, A. P., Park, C. Y., Corn, J. E., Kampmann, M., et al. (2016) · eLife · doi:10.7554/elife.19760 · Cited by 894
Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
Zetsche, B., Heidenreich, M., Mohanraju, P., Fedorova, I., Kneppers, J., DeGennaro, E. M., Winblad, N., Choudhury, S. R., Abudayyeh, O. O., Gootenberg, J. S., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3737 · Cited by 926
Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications
Liu, C., Zhang, L., Liu, H., & Cheng, K. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.09.012 · Cited by 607
Combinatorial CRISPR–Cas9 screens for de novo mapping of genetic interactions
Shen, J. P., Zhao, D., Sasik, R., Luebeck, J., Birmingham, A., Bojorquez-Gomez, A., Licon, K., Klepper, K., Pekin, D., Beckett, A. N., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4225 · Cited by 359
Viral Delivery Systems for CRISPR
Xu, C. L., Ruan, M. Z. C., Mahajan, V. B., & Tsang, S. H. (2019) · Viruses · doi:10.3390/v11010028 · Cited by 277
Lau, C.-H., & Suh, Y. (2017) · F1000Research · doi:10.12688/f1000research.11243.1 · Cited by 161
Viral Vectors for Gene Transfer
Chen, Y. H., Keiser, M. S., & Davidson, B. L. (2018) · Current Protocols in Mouse Biology · doi:10.1002/cpmo.58 · Cited by 146
One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment
Grav, L. M., Lee, J. S., Gerling, S., Kallehauge, T. B., Hansen, A. H., Kol, S., Lee, G. M., Pedersen, L. E., & Kildegaard, H. F. (2015) · Biotechnology Journal · doi:10.1002/biot.201500027 · Cited by 131
The potential of organoids in urological cancer research
Wang, S., Gao, D., & Chen, Y. (2017) · Nature Reviews Urology · doi:10.1038/nrurol.2017.65 · Cited by 87
Gao, Z., Harwig, A., Berkhout, B., & Herrera-Carrillo, E. (2017) · Transcription · doi:10.1080/21541264.2017.1322170 · Cited by 47
Vijayraghavan, S., & Kantor, B. (2017) · Journal of Visualized Experiments · doi:10.3791/56915 · Cited by 20
Metadata
- Authors
- Kabadi, A. M., Ousterout, D. G., Hilton, I. B., & Gersbach, C. A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-08-13
- Volume
- 42
- Issue
- 19
- Pages
- e147-e147
- ISSN
- 1362-4962
- 0305-1048
- PMC
- PMC4231726
- Cited by
- 370
- References
- 14
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review