Preputial Separation as an External Sign of Pubertal Development in the Male Rat1
Abstract
The separation of the prepuce from the glans penis, termed preputial separation, has been shown to be androgen dependent and to occur around the time of puberty in rats. In the present studies the mean ages of preputial separation in four experiments were 39, 42, 44 and 45 days of age, just preceding the appearance of mature sperm in the caput epididymis. Preputial separation occurred prior to the significant increase in circulating androgen levels by Day 51 as measured by radioimmunoassay. However, castration as late as Day 35 blocked preputial separation demonstrating that separation of the prepuce was dependent on the continued presence of a low level of androgen. Silastic implants of testosterone or dihydrotestosterone, but not estradiol, reverse the effects of castration. Handling neonatal males every day prior to weaning significantly delayed preputial separation and this delay was correlated with significantly lower circulating levels of androgen and LH at 45 days of age. These data show that preputial separation, an easily determined external sign of sexual development in male rats may be used as an index of change in peripubertal androgen secretion.
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In males, increased testosterone at puberty induces separation of the prepuce from the glans penis accompanied by increasing testicular volumes and eventual attainment of fertility.
“In males, increased testosterone at puberty induces separation of the prepuce from the glans penis (preputial separation; (Korenbrot et al., 1977) accompanied by increasing testicular volumes and eventual attainment of fertility.”
From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Korenbrot et al., 1977
Cites
DÖHLER, K.-D., & Wuttke, W. (1975) · Endocrinology · doi:10.1210/endo-97-4-898 · Cited by 729 · see citing works
Karsch, F. J., Dierschke, D. J., Weick, R. F., YAMAJI, T., Hotchkiss, J., & Knobil, E. (1973) · Endocrinology · doi:10.1210/endo-92-3-799 · Cited by 283 · see citing works
Denenberg, V. H., & ZARROW, M. X. (1971) · Elsevier eBooks · doi:10.1016/b978-0-12-731750-2.50011-0 · Cited by 121 · see citing works
Podesta, E. J., Rivarola, M. A., & Jyujo, T. (1974) · Endocrinology · doi:10.1210/endo-95-2-455 · Cited by 109 · see citing works
SELMANOFF, M. K., Goldman, B. D., & Ginsburg, B. E. (1977) · Endocrinology · doi:10.1210/endo-100-1-122 · Cited by 92 · see citing works
Gupta, D., RAGER, K., ZARZYCKI, J., & Eichner, M. (1975) · Journal of Endocrinology · doi:10.1677/joe.0.0660183 · Cited by 72 · see citing works
Effects of Age and Experience on Plasma Testosterone
Grota, L. J. (2008) · Neuroendocrinology · doi:10.1159/000122001 · Cited by 48 · see citing works
LYONS, W. R., Berlin, I. N., & FRIEDLANDER, S. (1942) · Endocrinology · doi:10.1210/endo-31-6-659 · Cited by 18 · see citing works
The Search for the Determinants of Puberty in the Rat
Moltz, H. (1975) · Springer eBooks · doi:10.1007/978-1-4615-7189-6_3 · Cited by 15 · see citing works
Effect of infantile stimulation on gonadal maturation of rats
Branchey, L., & Schiavi, R. C. (1968) · Journal of Psychosomatic Research · doi:10.1016/0022-3999(68)90046-9 · Cited by 6 · see citing works
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Koss, W. A., Belden, C. E., Hristov, A. D., & Juraska, J. M. (2014) · Synapse · doi:10.1002/syn.21716 · Cited by 228 · see citing works
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“Although androgen-dependent physiological changes (preputial separation) at adolescence are reported in male Sprague-Dawley rats as early as day postnatal day 38 [54] they are more commonly reported between postnatal days 45 and 48 [55].”
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Pereira, O. C. M., & Piffer, R. C. (2005) · Life Sciences · doi:10.1016/j.lfs.2005.04.001 · Cited by 18 · see citing works
Granata, L., Gildawie, K. R., Ismail, N., Brenhouse, H. C., & Kopec, A. M. (2022) · Developmental Cognitive Neuroscience · doi:10.1016/j.dcn.2022.101143 · Cited by 15 · see citing works
- Authors
- Korenbrot, C. C., Huhtaniemi, I. T., & Weiner, R. I.
- Affiliations
- University of California, San Francisco (US)
- ORCID
- Huhtaniemi, I. T.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1977-09-01
- Volume
- 17
- Issue
- 2
- Pages
- 298-303
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W2059223117
- Cited by
- 487
- References
- 10
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 8 · 2023: 11 · 2022: 28 · 2021: 13 · 2020: 33 · 2019: 15 · 2018: 14 · 2017: 26 · 2016: 28 · 2015: 22 · 2014: 27 · 2013: 23 · 2012: 15
- FWCI
- 4.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biology of Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
- Keywords
- preputial separation, puberty, male reproductive development, androgens, testosterone, dihydrotestosterone, castration, luteinizing hormone, neonatal handling
- MeSH terms
- Age Factors, Animals, Body Weight, Castration, Estradiol, Handling, Psychological, Luteinizing Hormone, Male, Penis, Sexual Maturation, Spermatogenesis, Dihydrotestosterone, Testosterone, Rats
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