Cited by 167Epigenetics and DNA MethylationFolate and B Vitamins ResearchNeurogenesis and neuroplasticity mechanisms

Choline deficiency alters global histone methylation and epigenetic marking at the Rel site of the calbindin 1 gene

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Mehedint, Mihai G., Niculescu, Mihai D., Craciunescu, Corneliu N., Zeisel, Steven H.·2010·The FASEB Journal

Abstract

Maternal choline availability is essential for fetal neurogenesis. Choline deprivation (CD) causes hypomethylation of specific CpG islands in genes controlling cell cycling in fetal hippocampus. We now report that, in C57BL/6 mice, CD during gestational days 12-17 also altered methylation of the histone H3 in E17 fetal hippocampi. In the ventricular and subventricular zones, monomethyl-lysine 9 of H3 (H3K9me1) was decreased by 25% (P<0.01), and in the pyramidal layer, dimethyl-lysine 9 of H3 (H3K9me2) was decreased by 37% (P<0.05). These changes were region specific and were not observed in whole-brain preparations. Also, the same effects of CD on H3 methylation were observed in E14 neural progenitor cells (NPCs) in culture. Changes in G9a histone methyltransferase might mediate altered H3K9me2,1. Gene expression of G9a was decreased by 80% in CD NPCs (P<0.001). In CD, H3 was hypomethylated upstream of the RE1 binding site in the calbindin 1 promoter, and 1 CpG site within the calbindin1 promoter was hypermethylated. REST binding to RE1 (recruits G9a) was decreased by 45% (P<0.01) in CD. These changes resulted in increased expression of calbindin 1 in CD (260%; P<0.05). Thus, CD modulates histone methylation in NPCs, and this could underlie the observed changes in neurogenesis.

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Crossref · 10.1096/fj.09-140145

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  • Diets deficient in choline and betaine directly affect histone and DNA methylation in several animal models.

    “Furthermore, diets deficient in choline and betaine were shown to directly affect histone and DNA methylation in a number animal models (Du et al., 2009; Mehedint et al., 2010; Niculescu et al., 2006), suggesting that alterations in exogenous methyl donor availability may have strong effects on cellular epigenetic state and thus contribute to tumorigenesis.”

    From The emerging hallmarks of cancer metabolism (2016) · cites this paper as Mehedint et al., 2010

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Authors
Mehedint, M. G., Niculescu, M. D., Craciunescu, C. N., & Zeisel, S. H.
Affiliations
University of North Carolina at Chapel Hill (US)
Corresponding author
Zeisel, Steven H.
Venue
The FASEB Journal
Publisher
Wiley
Published
2009-09-14
Volume
24
Issue
1
Pages
184-195
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
OpenAlex
W2070463636
Cited by
167
References
77
Citations by year
2026: 3 · 2025: 1 · 2024: 9 · 2023: 2 · 2022: 13 · 2021: 12 · 2020: 12 · 2019: 11 · 2018: 4 · 2017: 15 · 2016: 11 · 2015: 8 · 2014: 12 · 2013: 7 · 2012: 27
FWCI
6.31
Type
journal article
Language
en
Open access
Locations
  • The FASEB Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (AG-09525, DK-55865)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Health Sciences › Medicine › Rheumatology › Folate and B Vitamins Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
choline deficiency, histone H3 methylation, H3K9me1, H3K9me2, G9a, rest, neural progenitor cells, fetal hippocampus, neurogenesis, epigenetic regulation, CpG methylation, fetal development
MeSH terms
RE1-Silencing Transcription Factor, Animals, Base Sequence, Binding Sites, Cells, Cultured, Choline Deficiency, DNA, Female, Hippocampus, Histones, Methylation, Mice, Inbred C57BL, Mitosis, Models, Biological, Pregnancy, Promoter Regions, Genetic, Histone-Lysine N-Methyltransferase, Repressor Proteins, Apoptosis, CpG Islands, Epigenesis, Genetic, Mice, Embryonic Stem Cells, Calbindins, S100 Calcium Binding Protein G, Calbindin 1

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