Acute hypoxia attenuates resistance exercise-induced ribosome signaling but does not impact satellite cell pool expansion in human skeletal muscle
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Abstract Cumulative evidence supports the hypothesis that hypoxia acts as a regulator of muscle mass. However, the underlying molecular mechanisms remain incompletely understood, particularly in human muscle. Here we examined the effect of hypoxia on signaling pathways related to ribosome biogenesis and myogenic activity following an acute bout of resistance exercise. We also investigated whether hypoxia influenced the satellite cell response to resistance exercise. Employing a randomized, crossover design, eight men performed resistance exercise in normoxia (FiO 2 21%) or normobaric hypoxia (FiO 2 12%). Muscle biopsies were collected in a time‐course manner (before, 0, 90, 180 min and 24 h after exercise) and were analyzed with respect to cell signaling, gene expression and satellite cell content using immunoblotting, RT‐qPCR and immunofluorescence, respectively. In normoxia, resistance exercise increased the phosphorylation of RPS6, TIF‐1A and UBF above resting levels. Hypoxia reduced the phosphorylation of these targets by ~37%, ~43% and ~ 67% throughout the recovery period, respectively ( p < .05 vs. normoxia). Resistance exercise also increased 45 S pre‐rRNA expression and mRNA expression of c‐Myc, Pol I and TAF‐1A above resting levels, but no differences were observed between conditions. Similarly, resistance exercise increased mRNA expression of myogenic regulatory factors throughout the recovery period and Pax7 + cells were elevated 24 h following exercise in mixed and type II muscle fibers, with no differences observed between normoxia and hypoxia. In conclusion, acute hypoxia attenuates ribosome signaling, but does not impact satellite cell pool expansion and myogenic gene expression following a bout of resistance exercise in human skeletal muscle.
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Acute normobaric hypoxia attenuates resistance-exercise-induced phosphorylation of ribosome-related signaling proteins UBF and TIF-1A.
“Finally, acute normobaric hypoxia has been shown to attenuate resistance-exercise-induced phosphorylation of ribosome-related signaling proteins (UBF and TIF-1A), though its chronic implications for translational capacity remain unresolved [172].”
From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [172]
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“Finally, acute normobaric hypoxia has been shown to attenuate resistance-exercise-induced phosphorylation of ribosome-related signaling proteins (UBF and TIF-1A), though its chronic implications for translational capacity remain unresolved [172].”
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- Authors
- Horwath, O., Nordström, F., von Walden, F., Apró, W., & Moberg, M.
- Affiliations
- Swedish School of Sport and Health Sciences (SE)
- Division of Pediatric Neurology, Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden
- Karolinska Institutet (SE)
- Corresponding author
- Moberg, Marcus
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2023-02-14
- Volume
- 37
- Issue
- 3
- Pages
- e22811-e22811
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W4320710599
- Cited by
- 6
- References
- 58
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The FASEB Journal (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › High Altitude and Hypoxia
- Keywords
- acute hypoxia, resistance training, ribosome biogenesis, satellite cells, skeletal muscle, myogenic regulatory factors, Pax7, S6 phosphorylation, UBF, pre-rRNA, c-Myc, muscle mass regulation
- MeSH terms
- Hypoxia, Humans, Male, Ribosomes, RNA, Messenger, Signal Transduction, Muscle, Skeletal, Satellite Cells, Skeletal Muscle, Resistance Training
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