Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle
Abstract
The aim of this study was to examine the time course activation of select myogenic (MRF4, Myf5, MyoD, myogenin) and metabolic (CD36, CPT1, HKII, and PDK4) genes after an acute bout of resistance (RE) or run (Run) exercise. Six RE subjects [25 ± 4 yr (mean ± SD), 74 ± 14 kg, 1.71 ± 0.11 m] and six Run subjects (25 ± 4 yr, 72 ± 5 kg, 1.81 ± 0.07 m, 63 ± 8 ml·kg−1·min−1) were studied. Eight muscle biopsies were taken from the vastus lateralis (RE) and gastrocnemius (Run) before, immediately after, and 1, 2, 4, 8, 12 and 24 h after exercise. RE increased mRNA of MRF4 (3.7- to 4.5-fold 2–4 h post), MyoD (5.8-fold 8 h post), myogenin (2.6- and 3.5-fold 8–12 h post), HKII (3.6- to 10.5-fold 2–12 h post), and PDK4 (14- to 26-fold 2–8 h post). There were no differences in Myf5, CD36, and CPT1 mRNA levels 0–24 h post-RE. Run increased mRNA of MyoD (5.0- to 8.0-fold), HKII (12- to 16-fold), and PDK4 (32- to 52-fold) at 8–12 h postexercise. There were no differences in MRF4, Myf5, myogenin, CD36 and CPT1 mRNA levels 0–24 h post-Run. These data indicate a myogenic and metabolic gene induction with RE and Run exercise. The timing of the gene induction is variable and generally peaks 4–8 h postexercise with all gene expression not significantly different from the preexercise levels by 24 h postexercise. These data provide basic information for the timing of human muscle biopsy samples for gene-expression studies involving exercise.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Gene profiles after endurance running and resistance exercise are remarkably comparable.
“Yang and co-workers (Yang et al., 2005) and Louis and colleagues (Louis et al., 2007) compared the acute time-course of mRNA content of metabolic, myogenic and proteolytic mRNA targets after bouts of divergent exercise and show gene profiles that are remarkably comparable for endurance running and resistance exercise.”
From Concurrent exercise training: do opposites distract? (2017) · cites this paper as Yang et al., 2005
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · see citing works
Transcriptional regulation of gene expression in human skeletal muscle during recovery from exercise
Pilegaard, H., Ordway, G. A., Saltin, B., & Neufer, P. D. (2000) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.2000.279.4.e806 · Cited by 563 · see citing works
Exercise training increases lipid metabolism gene expression in human skeletal muscle
Tunstall, R. J., Mehan, K. A., Wadley, G. D., Collier, G. R., Bonen, A., Hargreaves, M., & Cameron-Smith, D. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00475.2001 · Cited by 292 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Integrative Biology of Exercise
Hawley, J. A., Hargreaves, M., Joyner, M. J., & Zierath, J. R. (2014) · Cell · doi:10.1016/j.cell.2014.10.029 · Cited by 1,152 · see citing works
The Molecular Bases of Training Adaptation
Coffey, V. G., & Hawley, J. A. (2007) · Sports Medicine · doi:10.2165/00007256-200737090-00001 · Cited by 756 · see citing works
Concurrent exercise training: do opposites distract?
Coffey, V. G., & Hawley, J. A. (2017) · The Journal of Physiology · doi:10.1113/jp272270 · Cited by 351 · see citing works
Fyfe, J. J., Bishop, D. J., & Stepto, N. K. (2014) · Sports Medicine · doi:10.1007/s40279-014-0162-1 · Cited by 347 · see citing works
Early signaling responses to divergent exercise stimuli in skeletal muscle from well-trained humans
Coffey, V. G., Zhong, Z., Shield, A., Canny, B. J., Chibalin, A. V., Zierath, J. R., & Hawley, J. A. (2006) · The FASEB Journal · doi:10.1096/fj.05-4809fje · Cited by 336 · see citing works
Egan, B., & Sharples, A. P. (2023) · Physiological Reviews · doi:10.1152/physrev.00054.2021 · Cited by 278 · see citing works
The role of exercise-induced myokines in regulating metabolism
Huh, J. Y. (2018) · Archives of Pharmacal Research · doi:10.1007/s12272-017-0994-y · Cited by 242 · see citing works
Bamman, M. M., Petrella, J. K., Kim, J.-S., Mayhew, D. L., & Cross, J. M. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00024.2007 · Cited by 216 · see citing works
Hawley, J. A. (2009) · Applied Physiology Nutrition and Metabolism · doi:10.1139/h09-023 · Cited by 203 · see citing works
Nutritional modulation of training-induced skeletal muscle adaptations
Hawley, J. A., Burke, L. M., Phillips, S. M., & Spriet, L. L. (2011) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00949.2010 · Cited by 205 · see citing works
Training with Low Muscle Glycogen Enhances Fat Metabolism in Well-Trained Cyclists
HULSTON, C. J., VENABLES, M. C., MANN, C. H., MARTIN, C., PHILP, A., BAAR, K., & JEUKENDRUP, A. E. (2010) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e3181dd5070 · Cited by 196 · see citing works
Rapid exercise-induced changes in PGC-1α mRNA and protein in human skeletal muscle
Mathai, A. S., Bonen, A., Benton, C. R., Robinson, D. L., & Graham, T. E. (2008) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00847.2007 · Cited by 158 · see citing works
Fatty acid-inducible ANGPTL4 governs lipid metabolic response to exercise
Catoire, M., Alex, S., Paraskevopulos, N., Mattijssen, F., Evers-van Gogh, I., Schaart, G., Jeppesen, J., Kneppers, A., Mensink, M., Voshol, P. J., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1400889111 · Cited by 150 · see citing works
Breen, L., Philp, A., Witard, O. C., Jackman, S. R., Selby, A., Smith, K., Baar, K., & Tipton, K. D. (2011) · The Journal of Physiology · doi:10.1113/jphysiol.2011.211888 · Cited by 143 · see citing works
Skeletal muscle nNOSμ protein content is increased by exercise training in humans
McConell, G. K., Bradley, S. J., Stephens, T. J., Canny, B. J., Kingwell, B. A., & Lee-Young, R. S. (2007) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00796.2006 · Cited by 136 · see citing works
Skeletal muscle gene expression in response to resistance exercise: sex specific regulation
Liu, D., Sartor, M. A., Nader, G. A., Gutmann, L., Treutelaar, M. K., Pistilli, E. E., IglayReger, H. B., Burant, C. F., Hoffman, E. P., & Gordon, P. M. (2010) · BMC Genomics · doi:10.1186/1471-2164-11-659 · Cited by 122 · see citing works
Coffey, V. G., Jemiolo, B., Edge, J., Garnham, A. P., Trappe, S. W., & Hawley, J. A. (2009) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00351.2009 · Cited by 120 · see citing works
Self-citationEgan, B., O’Connor, P. L., Zierath, J. R., & O’Gorman, D. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0074098 · Cited by 120 · see citing works
Catoire, M., Mensink, M., Boekschoten, M. V., Hangelbroek, R., Müller, M., Schrauwen, P., & Kersten, S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0051066 · Cited by 116 · see citing works
Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle
Coffey, V. G., Pilegaard, H., Garnham, A. P., O'Brien, B. J., & Hawley, J. A. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.91221.2008 · Cited by 116 · see citing works
Resistance exercise-induced changes of inflammatory gene expression within human skeletal muscle
Buford, T. W., Cooke, M. B., & Willoughby, D. S. (2009) · European Journal of Applied Physiology · doi:10.1007/s00421-009-1145-z · Cited by 111 · see citing works
Civitarese, A. E., Hesselink, M. K. C., Russell, A. P., Ravussin, E., & Schrauwen, P. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00193.2005 · Cited by 99 · see citing works
Effects of sleeping with reduced carbohydrate availability on acute training responses
Lane, S. C., Camera, D. M., Lassiter, D. G., Areta, J. L., Bird, S. R., Yeo, W. K., Jeacocke, N. A., Krook, A., Zierath, J. R., Burke, L. M., et al. (2015) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00857.2014 · Cited by 102 · see citing works
Churchley, E. G., Coffey, V. G., Pedersen, D. J., Shield, A., Carey, K. A., Cameron-Smith, D., & Hawley, J. A. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01260.2006 · Cited by 80 · see citing works
Pugh, J. K., Faulkner, S. H., Jackson, A. P., King, J. A., & Nimmo, M. A. (2015) · Physiological Reports · doi:10.14814/phy2.12364 · Cited by 63 · see citing works
Effects of skeletal muscle energy availability on protein turnover responses to exercise
Smiles, W. J., Hawley, J. A., & Camera, D. M. (2016) · Journal of Experimental Biology · doi:10.1242/jeb.125104 · Cited by 48 · see citing works
Kuang, J., McGinley, C., Lee, M. J.-C., Saner, N. J., Garnham, A., & Bishop, D. J. (2022) · PeerJ · doi:10.7717/peerj.12856 · Cited by 45 · see citing works
Hawley, J. A., Gibala, M. J., & Bermon, S. (2007) · Journal of Sports Sciences · doi:10.1080/02640410701607411 · Cited by 43 · see citing works
Mazo, C. E., D’Lugos, A. C., Sweeney, K. R., Haus, J. M., Angadi, S. S., Carroll, C. C., & Dickinson, J. M. (2021) · European Journal of Applied Physiology · doi:10.1007/s00421-021-04758-6 · Cited by 36 · see citing works
Dalbo, V. J., Roberts, M. D., Hassell, S., & Kerksick, C. M. (2013) · European Journal of Nutrition · doi:10.1007/s00394-012-0349-x · Cited by 35 · see citing works
Pugh, J. K., Faulkner, S. H., Turner, M. C., & Nimmo, M. A. (2018) · European Journal of Applied Physiology · doi:10.1007/s00421-017-3721-y · Cited by 27 · see citing works
Howard, E. E., Margolis, L. M., Berryman, C. E., Lieberman, H. R., Karl, J. P., Young, A. J., Montano, M. A., Evans, W. J., Rodriguez, N. R., Johannsen, N. M., et al. (2020) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00157.2020 · Cited by 28 · see citing works
Gallagher, P. M., Touchberry, C. D., Teson, K., McCabe, E., Tehel, M., & Wacker, M. J. (2013) · Applied Physiology Nutrition and Metabolism · doi:10.1139/apnm-2012-0301 · Cited by 17 · see citing works
Horwath, O., Nordström, F., von Walden, F., Apró, W., & Moberg, M. (2023) · The FASEB Journal · doi:10.1096/fj.202202065rr · Cited by 6 · see citing works
- Authors
- Yang, Y., Creer, A., Jemiolo, B., & Trappe, S.
- Affiliations
- Ball State University (US)
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 2005-05
- Volume
- 98
- Issue
- 5
- Pages
- 1745-1752
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2187195351
- Cited by
- 290
- References
- 3
- Citations by year
- 2026: 4 · 2025: 15 · 2024: 12 · 2023: 14 · 2022: 14 · 2021: 6 · 2020: 10 · 2019: 15 · 2018: 12 · 2017: 13 · 2016: 13 · 2015: 16 · 2014: 18 · 2013: 13 · 2012: 13
- FWCI
- 2.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agencia Estatal de Investigación
- National Institute on Aging (AG 18409, R01AG018409)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- acute exercise, resistance training, running, skeletal muscle gene expression, myogenic genes, metabolic genes, MyoD, myogenin, MRF4, Myf5, CD36, carnitine palmitoyltransferase 1, hexokinase 2, PDK4, vastus lateralis, gastrocnemius
- MeSH terms
- Adult, Female, Gene Expression Regulation, Humans, Male, Muscle Proteins, RNA, Messenger, Time Factors, Exercise, Transcriptional Activation, Muscle, Skeletal
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
