Cited by 185Acute Ischemic Stroke ManagementNeuroinflammation and Neurodegeneration MechanismsCell Adhesion Molecules Research

Antibodies against adhesion molecules reduce apoptosis after transient middle cerebral artery occlusion in rat brain

Chopp, Michael, Li, Yi, Jiang, Ning, Zhang, Rui Lan, Prostak, Jonathan·1996·Journal of Cerebral Blood Flow & Metabolism

Abstract

We tested the hypothesis that treatment of transient focal cerebral ischemia in rat with antibodies directed against adhesion molecules reduces apoptosis. Rats (n = 31) were subjected to 2 h of middle cerebral artery (MCA) occlusion induced by intraluminal insertion of a nylon monofilament into the internal carotid artery. Upon reperfusion, animals were treated with monoclonal antibodies directed against intercellular adhesion molecule (ICAM)-1) (n = 8) or integrin CD11b/CD18 (n = 10), or administered IgG1 as a control (n = 13). At 48 h after ischemia, animals were killed and the brains analyzed for ischemic cell damage, using hematoxylin and eosin (H/E); apoptosis, using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) method; and inflammatory cells, using immunohistochemistry with an anti-myeloperoxidase (MPO) antibody. Data revealed a significant reduction in the volume of infarction (p < 0.01) and a decline in the absolute (p < 0.001), and normalized (to the ischemic areas, p < 0.05) numbers of apoptotic cells in both animals treated with anti-ICAM-1 and anti-CD11b antibodies compared to control animals. The numbers of immunoreactive MPO cells were also reduced in the treatment groups compared to those in the control group (p < 0.05). These data suggest that treatment with anti-adhesion molecule antibodies selectively reduce apoptosis, and that a contributing factor to the beneficial effect of antibody treatment for reducing ischemic cell damage may be a reduction in numbers of apoptotic cells.

Integrity signals

Registry

Crossref · 10.1097/00004647-199607000-00007

Cites

76 of 76 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Chopp, M., Li, Y., Jiang, N., Zhang, R. L., & Prostak, J.
Affiliations
Henry Ford Health (US)
Venue
Journal of Cerebral Blood Flow & Metabolism
Published
1996-07
Volume
16
Issue
4
Pages
578-584
ISSN
  • 0271-678X (ISSN-L)
  • 1559-7016
OpenAlex
W2056824541
Cited by
185
References
76
Citations by year
2025: 1 · 2022: 3 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 6 · 2013: 6 · 2012: 3
FWCI
13.52
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Hort Innovation
  • National Institutes of Health (5r01ns034184-04, 5r01ns033627-08)
  • National Institute of Neurological Disorders and Stroke (R01NS-33627, P01NS023393, R01NS-34184)
Topics
  • Health Sciences › Medicine › Epidemiology › Acute Ischemic Stroke Management
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
adhesion molecules, apoptosis, transient middle cerebral artery occlusion, focal cerebral ischemia, ICAM-1, CD11b/CD18 integrin, infarct size, ischemia-reperfusion injury, neuroprotection
MeSH terms
Animals, Antibodies, Monoclonal, Arterial Occlusive Diseases, Brain, Cell Count, Cerebrovascular Disorders, Male, Peroxidase, Cell Adhesion Molecules, Macrophage-1 Antigen, Apoptosis, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review