Lactate is a Preferential Oxidative Energy Substrate over Glucose for Neurons in Culture
Open accessAbstract
The authors investigated concomitant lactate and glucose metabolism in primary neuronal cultures using 13 C- and 1 H-NMR spectroscopy. Neurons were incubated in a medium containing either [1- 13 C]glucose and different unlabeled lactate concentrations, or unlabeled glucose and different [3- 13 C]lactate concentrations. Overall, 13 C-NMR spectra of cellular extracts showed that more 13 C was incorporated into glutamate when lactate was the enriched substrate. Glutamate 13 C-enrichment was also found to be much higher in lactate-labeled than in glucose-labeled conditions. When glucose and lactate concentrations were identical (5.5 mmol/L), relative contributions of glucose and lactate to neuronal oxidative metabolism amounted to 21% and 79%, respectively. Results clearly indicate that when neurons are in the presence of both glucose and lactate, they preferentially use lactate as their main oxidative substrate.
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The original ANLS has been controversially discussed but has also gained considerable experimental support by in vitro and in vivo studies.
“While the original ANLS has been controversially discussed (Albanese et al., 2016;Bosoi & Rose, 2014;Dienel, 2017;Liguori et al., 2015;Yellen, 2018), it has also gained considerable experimental support by in vitro (Bouzier-Sore et al., 2003;Karagiannis et al., 2021) and in vivo studies (Mächler et al., 2016;Suzuki et al., 2011;Wyss et al., 2011;Zuend et al., 2020).”
From Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons (2024) · cites this paper as Bouzier-Sore et al., 2003
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- Authors
- Bouzier-Sore, A.-K., Voisin, P., Canioni, P., Magistretti, P. J., & Pellerin, L.
- Affiliations
- Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS/Université, Bordeaux, France
- Centre National de la Recherche Scientifique (FR)
- Institut de Physiologie, Lausanne, Switzerland
- Venue
- Journal of Cerebral Blood Flow & Metabolism
- Publisher
- SAGE Publications
- Published
- 2003-11
- Volume
- 23
- Issue
- 11
- Pages
- 1298-1306
- ISSN
- 0271-678X (ISSN-L)
- 1559-7016
- OpenAlex
- W2107450931
- Cited by
- 328
- References
- 1
- Citations by year
- 2026: 13 · 2025: 12 · 2024: 10 · 2023: 11 · 2022: 11 · 2021: 10 · 2020: 9 · 2019: 9 · 2018: 17 · 2017: 14 · 2016: 17 · 2015: 24 · 2014: 28 · 2013: 9 · 2012: 16
- FWCI
- 5.50
- Type
- journal article
- Language
- en
- Open access
- green
- infoscience.epfl.ch
- Locations
- Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- lactate, glucose, neuronal energy metabolism, oxidative metabolism, substrate preference, glutamate, 13C NMR, primary neuronal cultures, lactate oxidation, brain metabolism
- MeSH terms
- Animals, Animals, Newborn, Carbon Isotopes, Cells, Cultured, Citric Acid Cycle, Energy Metabolism, Glucose, Lactates, Neurons, Oxidative Phosphorylation, Rats, Wistar, Glutamic Acid, Nuclear Magnetic Resonance, Biomolecular, Rats
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