Cited by 242Oral microbiology and periodontitis researchBacterial biofilms and quorum sensingMicrobial infections and disease research

Fusobacterium nucleatum supports the growth of Porphyromonas gingivalis in oxygenated and carbon-dioxide-depleted environments

Diaz, P. I, Zilm, P. S, Rogers, A. H·2002·Microbiology

Abstract

The authors compared the differences in tolerance to oxygen of the anaerobic periodontopathic bacteria Fusobacterium nucleatum and Porphyromonas gingivalis, and explored the possibility that F. nucleatum might be able to support the growth of P. gingivalis in aerated and CO 2-depleted environments. Both micro-organisms were grown as monocultures and in co-culture in the presence and absence of CO 2 and under different aerated conditions using a continuous culture system. At steady state, viable counts were performed and the activities of the enzymes superoxide dismutase and NADH oxidase/peroxidase were assayed in P. gingivalis. In co-culture,F. nucleatum was able to support the growth of P. gingivalis in aerated and CO 2-depleted environments in which P. gingivalis, as a monoculture, was not able to survive.F. nucleatum not only appeared to have a much higher tolerance to oxygen than P. gingivalis, but a significant increase in its numbers occurred under moderately oxygenated conditions.F. nucleatum might have an additional indirect role in dental plaque maturation, contributing to the reducing conditions necessary for the survival of P. gingivalis and possibly other anaerobes less tolerant to oxygen. Additionally,F. nucleatum is able to generate a capnophilic environment essential for the growth of P. gingivalis.

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Registry

Crossref · 10.1099/00221287-148-2-467

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  • Fusobacterium nucleatum and A actinomycetemcomitans are well recognized in periodontal disease, and F nucleatum is noted for its role in the development of anaerobic microenvironments that facilitate the outgrowth of anaerobic periodontal pathogens.

    “Fusobacterium nucleatum and A actinomycetemcomitans are well recognized in periodontal disease,220, 221 and F nucleatum is noted for its role in the development of anaerobic microenvironments that facilitate the outgrowth of anaerobic periodontal pathogens.222”

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Research works printed but not linked: 1

  1. Massol-Deya, 1994, Biofilm architecture: a fortuitous engineering feature, ASM News, 60, 406

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Authors
Diaz, P. I., Zilm, P. S., & Rogers, A. H.
Affiliations
Adelaide University (AU)
Corresponding author
Rogers, A. H
Venue
Microbiology
Published
2002-02-01
Volume
148
Issue
2
Pages
467-472
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
OpenAlex
W2125265838
Cited by
241
References
23
Citations by year
2026: 8 · 2025: 20 · 2024: 16 · 2023: 19 · 2022: 12 · 2021: 18 · 2020: 18 · 2019: 19 · 2018: 12 · 2017: 6 · 2016: 11 · 2015: 11 · 2014: 10 · 2013: 3 · 2012: 10
FWCI
5.95
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
Funders
Australian Dental Research Foundation
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Fusobacterium nucleatum, Porphyromonas gingivalis, bacterial co-culture, oxygen tolerance, periodontal pathogens, superoxide dismutase, NADH oxidase, peroxidase, anaerobic bacteria
MeSH terms
Adaptation, Physiological, Anaerobiosis, Carbon Dioxide, Dental Plaque, Humans, Microscopy, Electron, Scanning, Multienzyme Complexes, NADH, NADPH Oxidoreductases, Oxygen, Peroxidases, Superoxide Dismutase, Porphyromonas gingivalis, Fusobacterium nucleatum, Biofilms

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