Streptococcus mutans Competence-Stimulating Peptide Inhibits Candida albicans Hypha Formation
Open accessAbstract
The oral cavity is colonized by microorganisms growing in biofilms in which interspecies interactions take place.Streptococcus mutans grows in biofilms on enamel surfaces and is considered one of the main etiological agents of human dental caries.Candida albicans is also commonly found in the human oral cavity, where it interacts with S. mutans. C. albicans is a polymorphic fungus, and the yeast-to-hypha transition is involved in virulence and biofilm formation. The aim of this study was to investigate interkingdom communication between C. albicans and S. mutans based on the production of secreted molecules.S. mutans UA159 inhibited C. albicans germ tube (GT) formation in cocultures even when physically separated from C. albicans. Only S. mutans spent medium collected in the early exponential phase (4-h-old cultures) inhibited the GT formation of C. albicans. During this phase,S. mutans UA159 produces a quorum-sensing molecule, competence-stimulating peptide (CSP). The role of CSP in inhibiting GT formation was confirmed by using synthetic CSP and a comC deletion strain of S. mutans UA159, which lacks the ability to produce CSP. Other S. mutans strains and other Streptococcus spp. also inhibited GT formation but to different extents, possibly reflecting differences in CSP amino acid sequences among Streptococcus spp. or differences in CSP accumulation in the media. In conclusion, CSP, an S. mutans quorum-sensing molecule secreted during the early stages of growth, inhibits the C. albicans morphological switch.
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Several studies have investigated dual-species biofilms comprising Candida albicans and a bacterium commonly isolated from denture stomatitis, periodontitis, or dental caries, such as Streptococcus mutans, Streptococcus gordonii, Actinomyces viscosus, or Fusobacterium species.
“Several studies have investigated such dual-species biofilms comprising C. albicans and a bacterium commonly isolated from denture stomatitis, periodontitis, or dental caries, such as Streptococcus mutans, Streptococcus gordonii, Actinomyces viscosus, or Fusobacterium species (12, 81, 82).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [82]
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- Authors
- Jarosz, L. M., Deng, D. M., van der Mei, H. C., Crielaard, W., & Krom, B. P.
- Affiliations
- University Medical Center Groningen (NL)
- Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Free University Amsterdam, Amsterdam, The Netherlands
- Department of Biomedical Engineering, the Kolff Institute, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands
- ORCID
- Krom, Bastiaan P.
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2009-11
- Volume
- 8
- Issue
- 11
- Pages
- 1658-1664
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- PMC
- PMC2772401
- OpenAlex
- W2103333884
- Cited by
- 187
- References
- 41
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 5 · 2023: 15 · 2022: 12 · 2021: 5 · 2020: 17 · 2019: 11 · 2018: 10 · 2017: 14 · 2016: 20 · 2015: 9 · 2014: 15 · 2013: 13 · 2012: 10
- FWCI
- 3.76
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Eukaryotic Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion)
- VU Research Portal (publishedVersion)
- UvA-DARE (University of Amsterdam) (submittedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Streptococcus mutans, Candida albicans, competence-stimulating peptide, germ tube formation, hyphal morphogenesis, yeast-to-hypha transition, quorum sensing, interspecies communication, dental caries, dental plaque, fungal-bacterial interactions
- MeSH terms
- Bacterial Proteins, Candida albicans, Humans, Streptococcal Infections, Streptococcus mutans, Down-Regulation, Protein Transport, Hyphae
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