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PreprintCited by 54Cellular transport and secretionPlant Molecular Biology ResearchLipid metabolism and biosynthesis

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Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant Specific Components

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Dahhan, Dana A., Reynolds, Gregory D., Cárdenas, Jessica J., Eeckhout, Dominique, Johnson, Alexander, Yperman, Klaas, Kaufmann, Walter A., Vang, Nou, Yan, Xu, Hwang, Inhwan, Heese, Antje, De Jaeger, Geert, Friml, Jiri, Van Damme, Daniel, Pan, Jianwei, Bednarek, Sebastian Y.·2021·The Plant Cell
DOI 10.1101/2021.09.16.460678PMC PMC9134090

Preprint — published as 10.1093/plcell/koac071 · Paper page

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Abstract

In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from the cell surface as well as the movement of cargo in post-Golgi trafficking pathways. This diversity of functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment and cargo selectivity. The adaptor- protein AP-1 complex operates as part of the secretory pathway at the trans -Golgi network, while the AP-2 complex and the TPLATE complex (TPC) jointly operate at the plasma membrane to execute clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated trafficking in plants will be the comprehensive identification and characterization of the network of evolutionarily conserved and plant- specific core and accessory machinery involved in the formation and targeting of CCVs. To facilitate these studies, we have analyzed the proteome of enriched trans -Golgi network/early endosome-derived and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis cells. Tandem mass spectrometry analysis results were validated by differential chemical labeling experiments to identify proteins co-enriching with CCVs. Proteins enriched in CCVs included previously characterized CCV components and cargos such as the vacuolar sorting receptors in addition to conserved and plant-specific components whose function in clathrin-mediated trafficking has not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis CCVs is further supported via additional biochemical data.

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Metadata

Authors
Dahhan, D. A., Reynolds, G. D., Cárdenas, J. J., Eeckhout, D., Johnson, A., Yperman, K., Kaufmann, W. A., Vang, N., Yan, X., Hwang, I., Heese, A., De Jaeger, G., Friml, J., Van Damme, D., Pan, J., & Bednarek, S. Y.
Affiliations
  • University of Wisconsin–Madison (US)
  • Ghent University (BE)
  • Institute of Science and Technology Austria (AT)
  • Lanzhou University (CN)
  • Pohang University of Science and Technology (KR)
  • University of Missouri (US)
Corresponding author
Bednarek, Sebastian Y.
ORCID
Venue
The Plant Cell
Publisher
openRxiv
Published
2021-09-17
Volume
34
Issue
6
Pages
2150-2173
ISSN
1040-4651 (ISSN-L)
DOI
10.1101/2021.09.16.460678
PMC
PMC9134090
OpenAlex
W4213418499
Cited by
54
References
1
Citations by year
2026: 7 · 2025: 6 · 2024: 9 · 2023: 15 · 2022: 16 · 2021: 1
FWCI
6.15
Type
journal article
Language
en
Open access
Locations
  • The Plant Cell (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Lanzhou University Institutional Repository (submittedVersion, cc-by-nc-sa)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
  • Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
Funders
  • National Science Foundation (1147032, 1121998, 1025837, 1614915)
  • Michigan State University
  • Institute of Science and Technology Austria
  • National Research Foundation
  • National Natural Science Foundation of China (31670283, 91754104, 31820103008)
  • Austrian Science Fund (I3630B25)
  • National Research Foundation of Korea (2019R1A2B5B03099982, No. 2019R1A2B5B03099982)
  • Universiteit Gent
  • College of Engineering, Michigan State University
  • Division of Integrative Organismal Systems (1025837)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
clathrin-coated vesicles, clathrin adaptor complexes, AP-1 complex, AP-2, clathrin-mediated endocytosis, trans-Golgi network, early endosomes, post-Golgi trafficking, vacuolar sorting receptor, plant-specific proteins, Arabidopsis thaliana, suspension-cultured cells
MeSH terms
Clathrin, Endocytosis, Arabidopsis, Transcription Factor AP-1, Proteome, Clathrin-Coated Vesicles, Proteomics

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