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PreprintCited by 40Extracellular vesicles in diseaseAdvanced Proteomics Techniques and ApplicationsProstate Cancer Treatment and Research

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Prostate Cancer Reshapes the Secreted and Extracellular Vesicle Urinary Proteomes

Open access
Khoo, Amanda, Govindarajan, Meinusha, Qiu, Zhuyu, Liu, Lydia Y., Ignatchenko, Vladimir, Waas, Matthew, Macklin, Andrew, Keszei, Alexander, Main, Brian P., Yang, Lifang, Lance, Raymond S., Downes, Michelle R., Semmes, O. John, Vesprini, Danny, Liu, Stanley K., Nyalwidhe, Julius O., Boutros, Paul C., Kislinger, Thomas·2023·Nature Communications
DOI 10.1101/2023.07.23.550214PMC PMC11176296

Preprint — published as 10.1038/s41467-024-49424-5 · Paper page

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Abstract

Urine is a complex biofluid that reflects both overall physiologic state and the state of the genitourinary tissues through which it passes. It contains both secreted proteins and proteins encapsulated in tissue-derived extracellular vesicles (EVs). To understand the population variability and clinical utility of urine, we quantified the secreted and EV proteomes from 190 men, including a subset with prostate cancer. We demonstrate that a simple protocol enriches prostatic proteins in urine. Secreted and EV proteins arise from different subcellular compartments. Urinary EVs are faithful surrogates of tissue proteomes, but secreted proteins in urine or cell line EVs are not. The urinary proteome is longitudinally stable over several years. It can accurately and non-invasively distinguish malignant from benign prostatic lesions, and can risk-stratify prostate tumors. This resource quantifies the complexity of the urinary proteome, and reveals the synergistic value of secreted and EV proteomes for translational and biomarker studies.

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Metadata

Authors
Khoo, A., Govindarajan, M., Qiu, Z., Liu, L. Y., Ignatchenko, V., Waas, M., Macklin, A., Keszei, A., Main, B. P., Yang, L., Lance, R. S., Downes, M. R., Semmes, O. J., Vesprini, D., Liu, S. K., Nyalwidhe, J. O., Boutros, P. C., & Kislinger, T.
Affiliations
  • University Health Network (CA)
  • University of California, Los Angeles (US)
  • Eastern Virginia Medical School (US)
  • Sunnybrook Health Science Centre (CA)
  • University of Toronto (CA)
Corresponding author
  • Boutros, Paul C.
  • Kislinger, Thomas
ORCID
Venue
Nature Communications
Publisher
openRxiv
Published
2023-07-25
Volume
15
Issue
1
Pages
5069-5069
ISSN
2041-1723 (ISSN-L)
DOI
10.1101/2023.07.23.550214
PMC
PMC11176296
OpenAlex
W4399622071
Cited by
40
References
1
Citations by year
2026: 18 · 2025: 18 · 2024: 4
FWCI
5.43
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • PubMed Central (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • U.S. Department of Health and Human Services (U01CA214194)
  • Prostate Cancer Foundation (U2CCA271894, 20CHAS01)
  • Prostate Cancer Canada
  • Hospital for Sick Children
  • National Institutes of Health (UM1TR004360)
  • Canadian Institutes of Health Research (PJT156357, 62384_1)
  • National Heart, Lung, and Blood Institute
  • National Institute of Mental Health
  • National Institute on Drug Abuse
  • National Human Genome Research Institute
  • NIH Office of the Director
  • National Cancer Institute (P20CA252717, U24CA248265, U01CA214194, U2CCA271894, R01CA272678)
  • National Institute of Neurological Disorders and Stroke
  • National Center for Advancing Translational Sciences
  • Common Fund
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Prostate Cancer Treatment and Research
Keywords
prostate cancer, urinary proteome, extracellular vesicles, secreted proteins, urinary biomarkers, non-invasive diagnosis, risk stratification, longitudinal stability, benign prostatic lesions

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