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PreprintCited by 4Bacterial Genetics and BiotechnologyCRISPR and Genetic EngineeringBiochemical and Structural Characterization

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Structure of a Type II SMC Wadjet Complex from Neobacillus vireti

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Roisné-Hamelin, Florian, Liu, Hon Wing, Gruber, Stephan·2025·Structure
DOI 10.1101/2025.03.10.642339

Preprint — published as 10.1016/j.str.2025.06.004

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Abstract

Summary: Structural Maintenance of Chromosomes proteins are essential DNA-folding motors that facilitate critical cellular functions, including chromosome segregation and DNA repair. Wadjet systems are prokaryotic SMC complexes specialized in cellular immunity against plasmids. Type I Wadjet systems restrict plasmids via a DNA extrusion-cleavage reaction. Two other Wadjet types (II and III) have also been identified, however, their molecular characteristics are unclear. Here, we reconstituted a representative type II Wadjet system from Neobacillus vireti . We show that this system shares substrate selection and cleavage properties with type I but exhibits distinctive structural features, including a long elbow-distal coiled coil, a channel-less hinge, and a tandem KITE subunit. These features help identify the common and distinguishing architectural elements in the family of Wadjet systems and raise intriguing questions about the evolution of prokaryotic SMC complexes.

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Metadata

Authors
Roisné-Hamelin, F., Liu, H. W., & Gruber, S.
Affiliations
University of Lausanne (CH)
Corresponding author
Gruber, Stephan
ORCID
Venue
Structure
Publisher
openRxiv
Published
2025-03-11
Volume
33
Issue
9
Pages
1498-1509.e5
ISSN
0969-2126 (ISSN-L)
DOI
10.1101/2025.03.10.642339
OpenAlex
W4412087254
Cited by
4
References
1
Citations by year
2025: 4
FWCI
2.33
Type
journal article
Language
en
Open access
Licence
Locations
  • Structure (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • IRIS (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion)
Funders
  • Washington D.C. Scottish Rite
  • European Molecular Biology Organization (ATLF 302-2022, ALTF 490-2021)
  • École Polytechnique Fédérale de Lausanne
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (10001017)
  • Université de Genève
  • European Research Council
  • H2020 European Research Council (724482)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
SMC complex, coiled coil, protein complex structure, DNA repair
MeSH terms
Bacillaceae, Bacterial Proteins, Chromosomal Proteins, Non-Histone, DNA, Bacterial, DNA, Circular, Cryoelectron Microscopy, Molecular Motor Proteins

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