The active spatial organization of the β-globin locus requires the transcription factor EKLF
Open accessAbstract
Three-dimensional organization of a gene locus is important for its regulation, as recently demonstrated for the β-globin locus. When actively expressed, the cis-regulatory elements of the β-globin locus are in proximity in the nuclear space, forming a compartment termed the Active Chromatin Hub (ACH). However, it is unknown which proteins are involved in ACH formation. Here, we show that EKLF, an erythroid transcription factor required for adult β-globin gene transcription, is also required for ACH formation. We conclude that transcription factors can play an essential role in the three-dimensional organization of gene loci.
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Several TFs were required for looping between the LCR and the distal genes, including GATA-1, FOG-1, EKLF and NLI/Ldb1.
“Following the discovery of chromatin looping in the β-globin locus, it was ascertained that several TFs were required for looping between the LCR and the distal genes, including GATA-1, FOG-1, EKLF and NLI/Ldb1 [315–317].”
From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [316]
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Cites
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- Authors
- Drissen, R., Palstra, R.-J., Gillemans, N., Splinter, E., Grosveld, F., Philipsen, S., & de Laat, W.
- Affiliations
- Erasmus MC (NL)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-10-15
- Volume
- 18
- Issue
- 20
- Pages
- 2485-2490
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC529536
- OpenAlex
- W2109264912
- Cited by
- 352
- References
- 4
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 6 · 2023: 8 · 2022: 6 · 2021: 9 · 2020: 6 · 2019: 13 · 2018: 16 · 2017: 14 · 2016: 17 · 2015: 19 · 2014: 19 · 2013: 22 · 2012: 26
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
- Funders
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Kruppel-like factors research
- Keywords
- beta-globin locus, EKLF, erythroid transcription factor, cis-regulatory elements, nuclear compartmentalization
- MeSH terms
- Animals, Chromatin, Cross-Linking Reagents, DNA, DNA-Binding Proteins, Formaldehyde, Gene Expression Regulation, Globins, Liver, Mice, Mutant Strains, Transcription Factors, Restriction Mapping, Polymerase Chain Reaction, DNA Primers, DNA, Complementary, Reverse Transcriptase Polymerase Chain Reaction, Mice, Kruppel-Like Transcription Factors
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