Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 665

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

NPAT links cyclin E–Cdk2 to the regulation of replication-dependent histone gene transcription

Open access
Zhao, Jiyong, Kennedy, Brian K., Lawrence, Brandon D., Barbie, David A., Matera, A. Gregory, Fletcher, Jonathan A., Harlow, Ed·2000·Genes & Development
DOI 10.1101/gad.827700
Open this paper in CoChat — free account

Abstract

In eukaryotic cells, histone gene expression is one of the major events that mark entry into S phase. While this process is tightly linked to cell cycle position, how it is regulated by the cell cycle machinery is not known. Here we show that NPAT, a substrate of the cyclin E–Cdk2 complex, is associated with human replication-dependent histone gene clusters on both chromosomes 1 and 6 in S phase. We demonstrate that NPAT activates histone gene transcription and that this activation is dependent on the promoter elements (SSCSs) previously proposed to mediate cell cycle–dependent transcription. Cyclin E is also associated with the histone gene loci, and cyclin E–Cdk2 stimulates the NPAT-mediated activation of histone gene transcription. Thus, our results both show that NPAT is involved in a key S phase event and provide a link between the cell cycle machinery and activation of histone gene transcription.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/gad.827700

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 1 references

Research works printed but not linked: 77

  1. 10.1007/s004390050630 · DOI
  2. 10.1016/0888-7543(91)90337-E · DOI
  3. 10.1083/jcb.142.4.899 · doi:10.1083/jcb.142.4.899
  4. 10.1084/jem.173.6.1407 · DOI · Paper page
  5. 10.1038/328823a0 · doi:10.1038/328823a0
  6. 10.1101/gad.7.4.592 · DOI · Paper page
  7. Clarke, 1992, Developmental regulation of chromatin composition during mouse embryogenesis: Somatic histone H1 is first detectable at the 4-cell stage., Development, 115, 791, 10.1242/dev.115.3.791 · doi:10.1242/dev.115.3.791
  8. 10.1016/S0168-9525(98)01621-7 · doi:10.1016/s0168-9525(98)01621-7
  9. Dailey, 1987, RNA polymerase II transcription factors H4TF-1 and H4TF-2 require metal to bind specific DNA sequences., Mol. Cell. Biol., 7, 4582
  10. 10.1101/gad.2.12b.1700 · DOI
  11. 10.1128/MCB.20.10.3715-3727.2000 · DOI
  12. Di Fruscio, 1997, Proviral inactivation of the Npat gene of Mpv 20 mice results in early embryonic arrest., Mol. Cell. Biol., 17, 4080, 10.1128/MCB.17.7.4080 · doi:10.1128/mcb.17.7.4080
  13. 10.1016/S0378-1119(99)00367-4 · DOI · Paper page
  14. Dominski, 1999, The stem-loop binding protein facilitates 3′ end formation by stabilizing U7 snRNP binding to the histone pre-mRNA., Mol. Cell. Biol., 19, 3561, 10.1128/MCB.19.5.3561 · DOI
  15. 10.1126/science.1329201 · DOI · Paper page
  16. 10.1016/0092-8674(94)90340-9 · DOI · Paper page
  17. 10.1016/0092-8674(91)90117-H · DOI · Paper page
  18. 10.1016/0092-8674(87)90100-0 · DOI · Paper page
  19. 10.1073/pnas.92.13.5915 · DOI · Paper page
  20. 10.1038/343437a0 · DOI · Paper page
  21. 10.1073/pnas.82.17.5685 · doi:10.1073/pnas.82.17.5685
  22. Hanly, 1985, Identification of promoter elements necessary for transcriptional regulation of a human histone H4 gene in vitro., Mol. Cell. Biol., 5, 380
  23. 10.1016/S0092-8674(00)81519-6 · DOI · Paper page
  24. Heintz, 1991, The regulation of histone gene expression during the cell cycle., Biochim. Biophys. Acta, 1088, 327, 10.1016/0167-4781(91)90122-3 · DOI
  25. 10.1073/pnas.93.21.11510 · DOI
  26. 10.1126/science.283.5403.851 · DOI · Paper page
  27. 10.1016/0092-8674(92)90249-C · DOI · Paper page
  28. 10.1073/pnas.89.1.305 · DOI
  29. 10.1083/jcb.130.4.755 · DOI · Paper page
  30. 10.1038/344830a0 · DOI · Paper page
  31. 10.1016/S1097-2765(00)80302-0 · DOI · Paper page
  32. 10.1126/science.1388288 · DOI · Paper page
  33. 10.1016/S0092-8674(00)81958-3 · DOI · Paper page
  34. 10.1101/gad.2.1.32 · DOI · Paper page
  35. 10.1101/gad.3.12a.1982 · doi:10.1101/gad.3.12a.1982
  36. 10.1073/pnas.96.6.2817 · DOI · Paper page
  37. Lam, 1993, An E2F-binding site mediates cell-cycle regulated repression of mouse B-myb transcription., EMBO J., 12, 2705, 10.1002/j.1460-2075.1993.tb05932.x · DOI · Paper page
  38. 10.1126/science.280.5363.547 · DOI · Paper page
  39. Liu, 2000, Cell cycle-dependent localization of the CDK2–cyclin E complex in Cajal (coiled) bodies., J. Cell Sci., 113, 1543, 10.1242/jcs.113.9.1543 · DOI
  40. 10.1016/S0092-8674(00)80940-X · DOI · Paper page
  41. Martinelli, 1994, H1TF2A, the large subunit of a heterodimeric, glutamine-rich CCAAT-binding transcription factor involved in histone H1 cell cycle regulation., Mol. Cell. Biol., 14, 8322
  42. Ma, T., Van Tine, B.A., Yue, W., Garrett, M., Nelson, D., Adams, P.D., Wang, J., Qin, J., Chow, L.T., and Harper, J.W. 2000. Cell cycle–regulated phosphorylation of p220NPAT by cyclin E/Cdk2 in cajal bodies promotes histone gene transcription. Genes & Dev. 14: (this issue).. · DOI · Paper page
  43. Marzluff, 1992, Histone 3′ ends: Essential and regulatory functions., Gene Expression., 2, 93
  44. 10.1016/S0962-8924(99)01606-2 · DOI · Paper page
  45. Melin, 1992, Biochemical demonstration of complex formation of histone pre-mRNA with U7 small unclear ribonucleoprotein and hairpin binding factors., EMBO J., 11, 691, 10.1002/j.1460-2075.1992.tb05101.x · DOI
  46. 10.1126/science.8384376 · DOI · Paper page
  47. Ohtsubo, 1995, Human cyclin E, a nuclear protein essential for the G1-to-S phase transition., Mol. Cell. Biol., 15, 2612, 10.1128/MCB.15.5.2612 · DOI · Paper page
  48. 10.1146/annurev.bi.60.070191.004143 · DOI · Paper page
  49. Oswald, 1996, The E2F transcription factor activates a replication-dependent human H2A gene in early S phase of the cell cycle., Mol. Cell. Biol., 16, 1889, 10.1128/MCB.16.5.1889 · DOI
  50. 10.1126/science.275.5299.523 · DOI · Paper page
  51. 10.1016/0092-8674(94)90572-X · DOI · Paper page
  52. 10.1073/pnas.91.10.4475 · DOI
  53. 10.1016/1047-8477(90)90066-L · DOI
  54. Resnitzky, 1994, Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system., Mol. Cell. Biol., 14, 1669
  55. 10.1073/pnas.57.2.409 · DOI · Paper page
  56. Ruffner, 1999, BRCA1 is phosphorylated at serine 1497 in vivo at a cyclin-dependent kinase 2 phosphorylation site., Mol. Cell. Biol., 19, 4843, 10.1128/MCB.19.7.4843 · DOI
  57. Schumperli, 1986, Cell-cycle regulation of histone gene expression., Cell, 45, 471, 10.1016/0092-8674(86)90277-1 · DOI · Paper page
  58. Seghezzi, 1998, Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells., Mol. Cell. Biol., 18, 4526, 10.1128/MCB.18.8.4526 · doi:10.1128/mcb.18.8.4526
  59. 10.1126/science.1684878 · DOI · Paper page
  60. Shanahan, 1999, Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI–SNF complex, and alters the ability of BRG1 to induce growth arrest., Mol. Cell. Biol., 19, 1460, 10.1128/MCB.19.2.1460 · DOI
  61. 10.1101/gad.11.11.1464 · DOI · Paper page
  62. 10.1093/nar/11.20.7069 · DOI
  63. Sive, 1986, Multiple sequence elements are required for maximal in vitro transcription of a human histone H2B gene., Mol. Cell. Biol., 6, 3329
  64. 10.1016/0955-0674(92)90028-B · doi:10.1016/0955-0674(92)90028-b
  65. 10.1016/0092-8674(94)90573-8 · DOI · Paper page
  66. Tsai, 1993, The cdk2 kinase is required for the G1-to-S transition in mammalian cells., Oncogene, 8, 1593
  67. 10.1126/science.8266103 · DOI · Paper page
  68. van Wijnen, 1992, Overlapping and CpG methylation-sensitive protein-DNA interactions at the histone H4 transcriptional cell cycle domain: Distinction between two human H4 gene promoters., Mol. Cell. Biol., 12, 3273
  69. 10.1073/pnas.93.21.11516 · DOI
  70. 10.1038/377362a0 · DOI · Paper page
  71. 10.1093/nar/13.8.2731 · DOI
  72. 10.1074/jbc.272.7.4483 · DOI
  73. Wiekowski, 1997, Changes in histone synthesis and modification at the beginning of mouse development correlate with the establishment of chromatin mediated repression of transcription., J. Cell Sci., 110, 1147, 10.1242/jcs.110.10.1147 · DOI
  74. 10.1093/nar/26.21.4837 · DOI
  75. 10.1074/jbc.270.32.18759 · DOI
  76. 10.1101/gad.12.4.456 · DOI · Paper page
  77. 10.1101/gad.9.14.1740 · DOI · Paper page

Cited by

See all 665

Metadata

Authors
Zhao, J., Kennedy, B. K., Lawrence, B. D., Barbie, D. A., Matera, A. G., Fletcher, J. A., & Harlow, E.
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
2000-09-15
Volume
14
Issue
18
Pages
2283-2297
ISSN
  • 0890-9369 (ISSN-L)
  • 1549-5477
DOI
10.1101/gad.827700
OpenAlex
W2100287099
Cited by
665
References
1
Type
posted content
Language
en
Open access
diamond

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: NPAT links cyclin E–Cdk2 to the regulation of replication-dependent histone gene transcription. https://app.cochat.ai/papers/10.1101/gad.827700
The paper
Zhao, J., Kennedy, B. K., Lawrence, B. D., Barbie, D. A., Matera, A. G., Fletcher, J. A., & Harlow, E. (2000). NPAT links cyclin E–Cdk2 to the regulation of replication-dependent histone gene transcription. Genes & Development. https://doi.org/10.1101/gad.827700

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.