The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
Open accessAbstract
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse Prkdc gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in F0 and F1 mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research.
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Citations
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Cited by
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CRISPR-mediated base editing in mice using cytosine deaminase base editor 4
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Metadata
- Authors
- Sung, Y. H., Kim, J. M., Kim, H.-T., Lee, J., Jeon, J., Jin, Y., Choi, J.-H., Ban, Y. H., Ha, S.-J., Kim, C.-H., Lee, H.-W., & Kim, J.-S.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2013-11-19
- Volume
- 24
- Issue
- 1
- Pages
- 125-131
- ISSN
- 1088-9051 (ISSN-L)
- OpenAlex
- W2138281066
- Cited by
- 283
- References
- 14
- Type
- posted content
- Language
- en
- Open access
- bronze
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