The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Silencing in Arabidopsis T-DNA Transformants: The Predominant Role of a Gene-Specific RNA Sensing Mechanism versus Position Effects
Open accessAbstract
Pronounced variability of transgene expression and transgene silencing are commonly observed among independent plant lines transformed with the same construct. Single-copy T-DNA lines harboring reporter genes of various kind and number under the control of a strong promoter were established in Arabidopsis thaliana for a comprehensive analysis of transgene expression. Characterization of 132 independent transgenic lines revealed no case of silencing as a result of site of T-DNA integration. Below a certain number of identical transgenes in the genome, gene copy number and expression were positively correlated. Expression was high, stable over all generations analyzed, and of a comparable level among independent lines harboring the same copy number of a particular transgene. Conversely, RNA silencing was triggered if the transcript level of a transgene surpassed a gene-specific threshold. Transcript level-mediated silencing effectively accounts for the pronounced transgene expression variability seen among transformants. It is proposed that the RNA sensing mechanism described is a genome surveillance system that eliminates RNA corresponding to excessively transcribed genes, including transgenes, and so plays an important role in genome defense.
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Cites
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Evolution of metal hyperaccumulation required cis-regulatory changes and triplication of HMA4
Hanikenne, M., Talke, I. N., Haydon, M. J., Lanz, C., Nolte, A., Motte, P., Kroymann, J., Weigel, D., & Krämer, U. (2008) · Nature · doi:10.1038/nature06877 · Cited by 812
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Rapp, R. A., & Wendel, J. F. (2005) · New Phytologist · doi:10.1111/j.1469-8137.2005.01491.x · Cited by 391
Regulating transgenic crops sensibly: Lessons from plant breeding, biotechnology and genomics
Bradford, K. J., Van Deynze, A., Gutterson, N., Parrott, W., & Strauss, S. H. (2005) · Nature Biotechnology · doi:10.1038/nbt1084 · Cited by 243
Salmon, J., Ward, S. P., Hanley, S. J., Leyser, O., & Karp, A. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12154 · Cited by 16
Metadata
- Authors
- Schubert, D., Lechtenberg, B., Forsbach, A., Gils, M., Bahadur, S., & Schmidt, R.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-09-14
- Volume
- 16
- Issue
- 10
- Pages
- 2561-2572
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2168626355
- Cited by
- 287
- References
- 45
- FWCI
- 19.87
- Type
- journal article
- Language
- en
- Open access
- green
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- Schubert, D., Lechtenberg, B., Forsbach, A., Gils, M., Bahadur, S., & Schmidt, R. (2004). Silencing in Arabidopsis T-DNA Transformants: The Predominant Role of a Gene-Specific RNA Sensing Mechanism versus Position Effects. The Plant Cell. https://doi.org/10.1105/tpc.104.024547
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