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Study of methanogen communities associated with different rumen protozoal populations
Open accessAbstract
Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganisms) were generated by sequential filtration. Holotrich-monofaunated sheep were also used to investigate the holotrich-associated methanogens. Protozoal size determined the number of PAM as big protozoa had 1.7-3.3 times more methanogen DNA than smaller protozoa, but also more endosymbiotic bacteria (2.2- to 3.5-fold times). Thus, similar abundance of methanogens with respect to total bacteria were observed across all protozoal fractions and free-living microorganisms, suggesting that methanogens are not accumulated within rumen protozoa in a greater proportion to that observed in the rumen as a whole. All rumen methanogen communities had similar diversity (22.2 ± 3.4 TRFs). Free-living methanogens composed a conserved community (67% similarity within treatment) in the rumen with similar diversity but different structures than PAM (P < 0.05). On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P < 0.001). Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.
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“rumen protozoal hydrogenosomes produce H2, which then serves as a substrate for methanogens to reduce CO2 to methane via the hydrogenotrophic pathway (Vogels et al., 1980; Belanche et al., 2014).”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Belanche et al., 2014
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Metadata
- Authors
- Belanche, A., de la Fuente, G., & Newbold, C. J.
- Affiliations
- Aberystwyth University (GB)
- Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK
- Corresponding author
- Newbold, Charles J.
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-12-01
- Volume
- 90
- Issue
- 3
- Pages
- 663-677
- ISSN
- 0168-6496 (ISSN-L)
- 1574-6941
- PMC
- PMC4286163
- OpenAlex
- W2018339579
- Cited by
- 147
- References
- 60
- Citations by year
- 2026: 6 · 2025: 16 · 2024: 20 · 2023: 11 · 2022: 11 · 2021: 19 · 2020: 12 · 2019: 16 · 2018: 10 · 2017: 7 · 2016: 10 · 2015: 8 · 2014: 1
- FWCI
- 17.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Ecology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- RECERCAT (Consorci de Serveis Universitaris de Catalunya) (publishedVersion, cc-by-nc)
- Repositori ObertUDL (University of Lleida) (publishedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc)
- Funders
- European Commission (FP7, REDNEX project)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- rumen methanogenesis, rumen protozoa, endosymbiotic bacteria, methane mitigation, sheep
- MeSH terms
- Animals, Bacteria, Methane, Polymorphism, Restriction Fragment Length, Rumen, Symbiosis, Trichostomatida, Euryarchaeota, Sheep, Domestic
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