Cited by 128Ovarian function and disordersSirtuins and Resveratrol in MedicineReproductive Biology and Fertility

Resveratrol treatment in patients with polycystic ovary syndrome decreased pro-inflammatory and endoplasmic reticulum stress markers

Brenjian, Samaneh, Moini, Ashraf, Yamini, Nazila, Kashani, Ladan, Faridmojtahedi, Maryam, Bahramrezaie, Mojdeh, Khodarahmian, Mahshad, Amidi, Fardin·2020·American Journal of Reproductive Immunology

Abstract

PROBLEM: Polycystic ovary syndrome (PCOS) is associated with endoplasmic reticulum (ER) stress and pro-inflammatory condition. The aim of the present study was to evaluate the effect of resveratrol treatment on pro-inflammatory and ER stress markers in patients with PCOS. METHOD OF STUDY: Cumulus cells were obtained from 40 patients with PCOS who were divided into two groups: placebo and resveratrol treatment (receiving 800 mg/d for 40 days) groups. Blood samples were obtained from all patients before and after the procedure to evaluate interleukin (IL)-6, IL-1β, IL-18, TNF-α, NF-κB, and C-reactive protein (CRP). Total RNA was extracted from cumulus cells, and cDNA was synthesized by reverse transcription. Expressions of five genes in ER stress response pathway (ATF4, ATF6, CHOP, GRP78, and XBP1s) were assessed with quantitative real-time PCR. Statistical analysis was performed with Student's t test. RESULTS: After treatment with resveratrol, it was found that serum levels of IL-6, IL-1β, TNF-α, IL-18, NF-κB, and CRP decreased in the treatment group. In addition, gene expression results showed that the expression levels of ATF4 (P < .05) and ATF6 (P < .001) significantly increased in the resveratrol treatment group, while the expression levels of CHOP, GRP78, and XBP1 (P < .001 for all) significantly decreased. CONCLUSION: Results demonstrated that resveratrol has anti-inflammatory effects through the suppression of NF-κB and NF-κB-regulated gene products. On the other hand, resveratrol can modulate ER stress in granulosa cells (GCs) by altering the expression of genes involved in unfolding protein response (UPR) process. Our findings suggest that ER stress is a potential therapeutic target for patients with PCOS.

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Crossref · 10.1111/aji.13186

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Authors
Brenjian, S., Moini, A., Yamini, N., Kashani, L., Faridmojtahedi, M., Bahramrezaie, M., Khodarahmian, M., & Amidi, F.
Affiliations
  • Tehran University of Medical Sciences (IR)
  • Royan Institute (IR)
  • University of Tehran (IR)
  • Department of Gynecology and Obstetrics School of Medicine Tehran University of Medical Science Tehran Iran
  • Shariati Hospital (IR)
Corresponding author
Amidi, Fardin
Venue
American Journal of Reproductive Immunology
Publisher
Wiley
Published
2019-11-03
Volume
83
Issue
1
Pages
e13186-e13186
ISSN
  • 0271-7352 (ISSN-L)
  • 1046-7408
  • 1600-0897
OpenAlex
W2972258394
Cited by
128
References
51
Citations by year
2026: 13 · 2025: 19 · 2024: 20 · 2023: 21 · 2022: 24 · 2021: 21 · 2020: 10
FWCI
13.11
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Reproductive Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Tehran University of Medical Sciences and Health Services (94‐03‐30‐29803)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
Keywords
polycystic ovary syndrome, resveratrol, endoplasmic reticulum stress, unfolded protein response, inflammation, cumulus cells, granulosa cells, NF-κB, inflammatory cytokines, ATF4, ATF6, CHOP, GRP78, XBP1, C-reactive protein
MeSH terms
X-Box Binding Protein 1, Resveratrol, Endoplasmic Reticulum Chaperone BiP, Adolescent, Adult, Anti-Inflammatory Agents, C-Reactive Protein, Cells, Cultured, Double-Blind Method, Female, Heat-Shock Proteins, Humans, Polycystic Ovary Syndrome, Cytokines, NF-kappa B, Activating Transcription Factor 4, Activating Transcription Factor 6, Transcription Factor CHOP, Cumulus Cells, Young Adult, Endoplasmic Reticulum Stress

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