Neutrophil activation by C andida glabrata but not C andida albicans promotes fungal uptake by monocytes: C. albicans , C. glabrata and neutrophils
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Candida albicans and Candida glabrata account for the majority of candidiasis cases worldwide. Although both species are in the same genus, they differ in key virulence attributes. Within this work, live cell imaging was used to examine the dynamics of neutrophil activation after confrontation with either C. albicans or C. glabrata. Analyses revealed higher phagocytosis rates of C. albicans than C. glabrata that resulted in stronger PMN (polymorphonuclear cells) activation by C. albicans. Furthermore, we observed differences in the secretion of chemokines, indicating chemotactic differences in PMN signalling towards recruitment of further immune cells upon confrontation with Candida spp. Supernatants from co-incubations of neutrophils with C. glabrata primarily attracted monocytes and increased the phagocytosis of C. glabrata by monocytes. In contrast, PMN activation by C. albicans resulted in recruitment of more neutrophils. Two complex infection models confirmed distinct targeting of immune cell populations by the two Candida spp.: In a human whole blood infection model, C. glabrata was more effectively taken up by monocytes than C. albicans and histopathological analyses of murine model infections confirmed primarily monocytic infiltrates in C. glabrata kidney infection in contrast to PMN-dominated infiltrates in C. albicans infection. Taken together, our data demonstrate that the human opportunistic fungi C. albicans and C. glabrata are differentially recognized by neutrophils and one outcome of this differential recognition is the preferential uptake of C. glabrata by monocytes.
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C. glabrata was preferentially taken up by monocytes in the whole blood infection model.
“Intriguingly, C. glabrata was preferentially taken up by monocytes in the whole blood infection model, and infiltration of predominantly monocytes was observed in mouse kidneys [143].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [143]
Infiltration of predominantly monocytes was observed in mouse kidneys.
“Intriguingly, C. glabrata was preferentially taken up by monocytes in the whole blood infection model, and infiltration of predominantly monocytes was observed in mouse kidneys [143].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [143]
C. glabrata-activated neutrophils secreted monocyte chemoattractants MIP-1α and MIP-1β, leading to increased migration of monocytes to the site of neutrophil-C. glabrata confrontation.
“Consistent with this, C. glabrata-activated neutrophils secreted monocyte chemoattractants, MIP-1α and MIP-1β, leading to increased migration of monocytes to the site of neutrophil-C. glabrata confrontation [143].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [143]
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Candida glabrata: A Lot More Than Meets the Eye
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- Authors
- Duggan, S., Essig, F., Hünniger, K., Mokhtari, Z., Bauer, L., Lehnert, T., Brandes, S., Häder, A., Jacobsen, I. D., Martin, R., Figge, M. T., & Kurzai, O.
- Affiliations
- Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI) (DE)
- Jena University Hospital (DE)
- Corresponding author
- Kurzai, Oliver
- ORCID
- Venue
- Cellular Microbiology
- Publisher
- Wiley
- Published
- 2015-05-05
- Volume
- 17
- Issue
- 9
- Pages
- 1259-1276
- ISSN
- 1462-5814 (ISSN-L)
- OpenAlex
- W1785625320
- Cited by
- 60
- References
- 50
- Citations by year
- 2026: 7 · 2025: 2 · 2024: 6 · 2023: 5 · 2022: 4 · 2021: 13 · 2020: 7 · 2019: 6 · 2018: 4 · 2017: 1 · 2016: 2 · 2015: 3
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cellular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Bristol Research (University of Bristol) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (CRC124-B4, C3)
- Federal Ministry for Science and Education (03Z2JN21)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida glabrata, Candida albicans, neutrophil activation, monocyte phagocytosis, chemokine secretion, neutrophil recruitment, candidiasis, fungal virulence, pyelonephritis, immune cell infiltration, host-pathogen interaction
- MeSH terms
- Animals, Candida albicans, Candidiasis, Cells, Cultured, Disease Models, Animal, Histocytochemistry, Humans, Kidney, Monocytes, Phagocytosis, Cytokines, Neutrophil Activation, Candida glabrata, Mice
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