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Trimethoprim resistance transposon Tn 4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS 257

Rouch, D. A., Messerotti, L. J., Loo, L. S. L., Jackson, C. A., Skurray, R. A.·1989·Molecular Microbiology
DOI 10.1111/j.1365-2958.1989.tb01805.x
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Abstract

Summary: Trimethoprim resistance mediated by the Staphylococcus aureus multi‐resistance plasmid pSK1 is encoded by a structure with characteristics of a composite transposon which we have designated Tn 4003. Nucleotide sequence analysis of Tn 4003 revealed it to be 4717 bp in length and to contain three copies of the insertion element IS 257 (789‐790 bp), the outside two of which are flanked by directly repeated 8‐bp target sequences. IS 257 has imperfect terminal inverted repeats of 27‐28 bp and encodes for a putative transposase with two potential α‐helix‐turn‐α‐helix DNA recognition motifs. IS 257 shares sequence similarities with members of the IS 15 family of insertion sequences from Gram‐negative bacteria and with ISS 1 from Streptococcus lactis. The central region of the transposon contains the dfrA gene that specifies the S1 dihydrofolate reductase (DHFR) responsible for trimethoprim resistance. The S1 enzyme shows sequence homology with type I and V trimethoprim‐resistant DHFRs from Gram‐negative bacteria and with chromosomally encoded DHFRs from Gram‐positive and Gram‐negative bacteria. 5’to dfrA is a thymidylate synthetase gene, designated thyE.

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Metadata

Authors
Rouch, D. A., Messerotti, L. J., Loo, L. S. L., Jackson, C. A., & Skurray, R. A.
Venue
Molecular Microbiology
Publisher
Wiley
Published
1989-02
Volume
3
Issue
2
Pages
161-175
ISSN
  • 0950-382X
  • 1365-2958
DOI
10.1111/j.1365-2958.1989.tb01805.x
Cited by
131
References
0
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 6477 d)

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CoChat. (2026). Citation check: Trimethoprim resistance transposon Tn 4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS 257. https://app.cochat.ai/papers/10.1111/j.1365-2958.1989.tb01805.x
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Rouch, D. A., Messerotti, L. J., Loo, L. S. L., Jackson, C. A., & Skurray, R. A. (1989). Trimethoprim resistance transposon Tn 4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS 257. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.1989.tb01805.x

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