Cited by 437Metabolism, Diabetes, and CancerCardiovascular and exercise physiologyMuscle metabolism and nutrition

The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.

Isoform‐specific and exercise intensity‐dependent activation of 5′‐AMP‐activated protein kinase in human skeletal muscle

Open access

Wojtaszewski, Jørgen F. P., Nielsen, Pernille, Hansen, Bo F., Richter, Erik A., Kiens, Bente·2000·The Journal of Physiology

DOI 10.1111/j.1469-7793.2000.t01-1-00221.xPMC PMC2270117

Open this paper in CoChat — free account

Abstract

1. 5'-AMP-activated protein kinase (AMPK) has been suggested to play a key role in the regulation of metabolism in skeletal muscle. AMPK is activated in treadmill-exercised and electrically stimulated rodent muscles. Whether AMPK is activated during exercise in humans is unknown. 2. We investigated the degree of activation and deactivation of alpha-isoforms of AMPK during and after exercise. Healthy human subjects performed bicycle exercise on two separate occasions at either a low ( approximately 50% maximum rate of O2 uptake (VO2,max) for 90 min) or a high ( approximately 75% VO2,max for 60 min) intensity. Biopsies from the vastus lateralis muscle were obtained before and immediately after exercise, and after 3 h of recovery. 3. We observed a 3- to 4-fold activation of the alpha2-AMPK isoform immediately after high intensity exercise, whereas no activation was observed after low intensity exercise. The activation of alpha2-AMPK was totally reversed 3 h after exercise. In contrast, alpha1-AMPK was not activated during either of the two exercise trials. 4. The in vitro AMP dependency of alpha2-AMPK was significantly greater than that of alpha1-AMPK ( approximately 3- vs. approximately 2-fold). 5. We conclude that in humans activation of alpha2-AMPK during exercise is dependent upon exercise intensity. The stable activation of alpha2-AMPK, presumably due to the activation of an upstream AMPK kinase, is compatible with a role for this kinase complex in the regulation of skeletal muscle metabolism during exercise, whereas the lack of stable alpha1-AMPK activation makes this kinase complex a less likely candidate.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1111/j.1469-7793.2000.t01-1-00221.x

Cites

26 of 26 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A Flexible System of Enzymatic Analysis
Lowry, O. H., & Passonneau, J. V. (1972) · Elsevier eBooks · doi:10.1016/b978-0-12-457950-7.x5001-3 · Cited by 4,050
THE AMP-ACTIVATED/SNF1 PROTEIN KINASE SUBFAMILY: Metabolic Sensors of the Eukaryotic Cell?
Hardie, D. G., Carling, D., & Carlson, M. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.821 · Cited by 1,440
AICA riboside increases AMP-activated protein kinase, fatty acid oxidation, and glucose uptake in rat muscle
Merrill, G. F., Kurth, E. J., Hardie, D. G., & Winder, W. W. (1997) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1997.273.6.e1107 · Cited by 1,048
AMP-activated protein kinase, a metabolic master switch: possible roles in Type 2 diabetes
Winder, W. W., & Hardie, D. G. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.277.1.e1 · Cited by 1,000
Inactivation of acetyl-CoA carboxylase and activation of AMP-activated protein kinase in muscle during exercise
Winder, W. W., & Hardie, D. G. (1996) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1996.270.2.e299 · Cited by 799
Evidence for 5' AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport
Hayashi, T., Hirshman, M. F., Kurth, E. J., Winder, W. W., & Goodyear, L. J. (1998) · Diabetes · doi:10.2337/diab.47.8.1369 · Cited by 774
Evidence for 5' AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport
Hayashi, T., Hirshman, M. F., Kurth, E. J., Winder, W. W., & Goodyear, L. J. (1998) · Diabetes · doi:10.2337/diabetes.47.8.1369 · Cited by 774
5' AMP-activated protein kinase activation causes GLUT4 translocation in skeletal muscle.
Kurth-Kraczek, E. J., Hirshman, M. F., Goodyear, L. J., & Winder, W. W. (1999) · Diabetes · doi:10.2337/diabetes.48.8.1667 · Cited by 715
Mammalian AMP-activated protein kinase subfamily
Stapleton, D., Mitchelhill, K. I., Gao, G., Widmer, J., Michell, B. J., Teh, T., House, C. M., Fernandez, C. S., Cox, T., Witters, L. A., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.2.611 · Cited by 652
Malonyl-CoA, fuel sensing, and insulin resistance
Ruderman, N. B., Saha, A. K., Vavvas, D. G., & Witters, L. A. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.276.1.e1 · Cited by 649

Show all 25 references

Research works printed but not linked: 2

Hayashi T., 2000, The alpha2 isoform of the 5′ AMP‐activated protein kinase may mediate contraction stimulated glucose transport in skeletal muscle, Diabetes, 48, A13
Ruderman N. B., 1999, Malonyl‐CoAfuel sensing, and insulin resistance, American Journal of Physiology, 276, E1

Sorted by citations

Items 101–200 of 437

AS160 phosphorylation is associated with activation of α 2 β 2 γ 1 - but not α 2 β 2 γ 3 -AMPK trimeric complex in skeletal muscle during exercise in humans
Treebak, J. T., Birk, J. B., Rose, A. J., Kiens, B., Richter, E. A., & Wojtaszewski, J. F. P. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00380.2006 · Cited by 133
Self-citation
Dissociation of AMPK activity and ACCβ phosphorylation in human muscle during prolonged exercise
Wojtaszewski, J. F. P., Mourtzakis, M., Hillig, T., Saltin, B., & Pilegaard, H. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02465-8 · Cited by 126
Self-citation
Role of exercise in maintaining the integrity of the neuromuscular junction
Nishimune, H., Stanford, J. A., & Mori, Y. (2013) · Muscle and Nerve · doi:10.1002/mus.24095 · Cited by 119
Molecular Adaptations to Aerobic Exercise Training in Skeletal Muscle of Older Women
Konopka, A. R., Douglass, M. D., Kaminsky, L. A., Jemioło, B., Trappe, T. A., Trappe, S. W., & Harber, M. P. (2010) · The Journals of Gerontology Series A · doi:10.1093/gerona/glq109 · Cited by 134
Skeletal muscle glucose uptake during contraction is regulated by nitric oxide and ROS independently of AMPK
Merry, T. L., Steinberg, G. R., Lynch, G. S., & McConell, G. K. (2010) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00239.2009 · Cited by 122
AMPK in cardiovascular health and disease
Shirwany, N. A., & Zou, M. (2010) · Acta Pharmacologica Sinica · doi:10.1038/aps.2010.139 · Cited by 123
Creatine supplementation increases glucose oxidation and AMPK phosphorylation and reduces lactate production in L6 rat skeletal muscle cells
Ceddia, R., & Sweeney, G. (2004) · Journal of Physiology · doi:10.1113/jphysiol.2003.056291 · Cited by 119
Using Molecular Biology to Maximize Concurrent Training
Baar, K. (2014) · Sports Medicine · doi:10.1007/s40279-014-0252-0 · Cited by 135
“This suggestion is based on the fact that AMPK activity increases rapidly and then returns to baseline levels within the first 3 h after high-intensity exercise [63], whereas mTORC1 activity can be maintained for at least 18 h after resistance exercise [8, 9].”
Background
Human muscle fibre type‐specific regulation of AMPK and downstream targets by exercise
Steenberg, D. E., Albers, P. H., Prats, C., Baba, O., Birk, J. B., & Wojtaszewski, J. F. P. (2015) · The Journal of Physiology · doi:10.1113/jphysiol.2014.283267 · Cited by 121
Self-citation
Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity
Kraniou, G. N., Cameron‐Smith, D., & Hargreaves, M. (2006) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01489.2005 · Cited by 117
The role of AMP-activated protein kinase in the coordination of skeletal muscle turnover and energy homeostasis
Sanchez, A. M. J., Candau, R. B., Csibi, A., Pagano, A. F., Raibon, A., & Bernardi, H. (2012) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00125.2012 · Cited by 122
PGC-1α is required for training-induced prevention of age-associated decline in mitochondrial enzymes in mouse skeletal muscle
Leick, L., Lyngby, S. S., Wojtaszewski, J. F. P., & Pilegaard, H. (2010) · Experimental Gerontology · doi:10.1016/j.exger.2010.01.011 · Cited by 121
Self-citation
Metabolic adaptations to fasting and chronic caloric restriction in heart, muscle, and liver do not include changes in AMPK activity
Gonzalez, A. A., Kumar, R., Mulligan, J. D., Davis, A. J., Weindruch, R. H., & Saupe, K. W. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00172.2004 · Cited by 118
AMP-Activated Protein Kinase: A Master Switch in Glucose and Lipid Metabolism
Hardie, D. G. (2004) · Reviews in Endocrine and Metabolic Disorders · doi:10.1023/b:remd.0000021433.63915.bb · Cited by 117
Effect of AICAR Treatment on Glycogen Metabolism in Skeletal Muscle
Aschenbach, W. G., Hirshman, M. F., FUJII, N. L., Sakamoto, K., Howlett, K. F., & Goodyear, L. J. (2002) · Diabetes · doi:10.2337/diabetes.51.3.567 · Cited by 114
Effect of Exercise Training on Fat Loss—Energetic Perspectives and the Role of Improved Adipose Tissue Function and Body Fat Distribution
Kolnes, K. J., Petersen, M. H., Lien-Iversen, T., Højlund, K., & Jensen, J. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.737709 · Cited by 112
Role of metabolic stress for enhancing muscle adaptations: Practical applications
de Freitas, M. C., Gerosa-Neto, J., Zanchi, N. E., Lira, F. S., & Rossi, F. E. (2017) · World Journal of Methodology · doi:10.5662/wjm.v7.i2.46 · Cited by 124
Exploring the Role of Genetic Variability and Lifestyle in Oxidative Stress Response for Healthy Aging and Longevity
Dato, S., Crocco, P., D’Aquila, P., Rango, F. D., Bellizzi, D., Rose, G., & Passarino, G. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms140816443 · Cited by 111
Resistance exercise induced mTORC1 signaling is not impaired by subsequent endurance exercise in human skeletal muscle
Apró, W., Wang, L., Pontén, M., Blomstrand, E., & Sahlin, K. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00091.2013 · Cited by 109
AMPK activation increases uncoupling protein-3 expression and mitochondrial enzyme activities in rat muscle without fibre type transitions
Putman, C. T., Kiricsi, M., Pearcey, J. A., MacLean, I. M., Bamford, J. A., Murdoch, G. K., Dixon, W. T., & Pette, D. (2003) · The Journal of Physiology · doi:10.1111/j.1469-7793.2003.00169.x · Cited by 110
Normal mammalian skeletal muscle and its phenotypic plasticity
Hoppeler, H. H., & Flück, M. (2002) · Journal of Experimental Biology · doi:10.1242/jeb.205.15.2143 · Cited by 111
Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1α mRNA: a role of β2-adrenergic receptor activation
Tadaishi, M., Miura, S., Kai, Y., Kawasaki, E., Koshinaka, K., Kawanaka, K., Nagata, J., Oishi, Y., & Ezaki, O. (2010) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00400.2010 · Cited by 109
Intravenous AICAR administration reduces hepatic glucose output and inhibits whole body lipolysis in type 2 diabetic patients
Boon, H., Bosselaar, M., Praet, S., Blaak, E. E., Saris, W. H. M., Wagenmakers, A. J. M., McGee, S. L., Tack, C. J., Smits, P., Hargreaves, M., et al. (2008) · Diabetologia · doi:10.1007/s00125-008-1108-7 · Cited by 108
Menopause is associated with decreased whole body fat oxidation during exercise
Abildgaard, J., Pedersen, A. T., Green, C., Harder-Lauridsen, N. M., Solomon, T. P. J., Thomsen, C., Juul, A., Pedersen, M., Pedersen, J. T., Mortensen, O. H., et al. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00492.2012 · Cited by 108
AMP-activated protein kinase activates transcription of the UCP3 and HKII genes in rat skeletal muscle
Stoppani, J., Hildebrandt, A. L., Sakamoto, K., Cameron‐Smith, D., Goodyear, L. J., & Neufer, P. D. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00278.2002 · Cited by 111
Endurance factors improve hippocampal neurogenesis and spatial memory in mice
Kobilo, T., Yuan, C., & van Praag, H. (2011) · Learning & Memory · doi:10.1101/lm.2001611 · Cited by 114
Dissociation of Increases in PGC-1α and Its Regulators from Exercise Intensity and Muscle Activation Following Acute Exercise
Edgett, B. A., Foster, W. S., Hankinson, P. B., Simpson, C. A., Little, J. P., Graham, R. B., & Gurd, B. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0071623 · Cited by 110
Effect of exercise intensity and hypoxia on skeletal muscle AMPK signaling and substrate metabolism in humans
Wadley, G. D., Lee, R. S., Canny, B. J., Wasuntarawat, C., Chen, Z. P., Hargreaves, M., Kemp, B. E., & McConell, G. K. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00464.2005 · Cited by 111
Interleukin-6 release from human skeletal muscle during exercise: relation to AMPK activity
MacDonald, C., Wojtaszewski, J. F. P., Pedersen, B. K., Kiens, B., & Richter, E. A. (2003) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00242.2003 · Cited by 105
Self-citation
A Ca2+–calmodulin–eEF2K–eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions
Rose, A. J., Alsted, T. J., Jensen, T. E., Kobberø, J. B., Maarbjerg, S., Jensen, J., & Richter, E. A. (2009) · The Journal of Physiology · doi:10.1113/jphysiol.2008.167528 · Cited by 106
Self-citation
Effect of fiber type and nutritional state on AICAR- and contraction-stimulated glucose transport in rat muscle
Ai, H., Ihlemann, J., Hellsten, Y., Lauritzen, H. P. M. M., Hardie, D. G., Galbo, H., & Ploug, T. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00167.2001 · Cited by 105
Role of AMP-Activated Protein Kinase in the Molecular Adaptation to Endurance Exercise
Winder, W. W., Taylor, E. B., & Thomson, D. M. (2006) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000233798.62153.50 · Cited by 102
Regulation of autophagy in human skeletal muscle: effects of exercise, exercise training and insulin stimulation
Fritzen, A. M., Madsen, A. B., Kleinert, M., Treebak, J. T., Lundsgaard, A., Jensen, T. E., Richter, E. A., Wojtaszewski, J. F. P., Kiens, B., & Frøsig, C. (2015) · The Journal of Physiology · doi:10.1113/jp271405 · Cited by 103
Self-citation
AICAR and metformin, but not exercise, increase muscle glucose transport through AMPK-, ERK-, and PDK1-dependent activation of atypical PKC
Sajan, M. P., Bandyopadhyay, G. K., Miura, A., Standaert, M. L., Nimal, S., Longnus, S., OBBERGHEN, E. V., Hainault, I., Foufelle, F., Kahn, R. C., et al. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00392.2009 · Cited by 99
AMPK and TBC1D1 Regulate Muscle Glucose Uptake After, but Not During, Exercise and Contraction
Kjøbsted, R., Roll, J. L. W., Jørgensen, N. O., Birk, J. B., Foretz, M., Viollet, B., Chadt, A., Al-Hasani, H., & Wojtaszewski, J. F. P. (2019) · Diabetes · doi:10.2337/db19-0050 · Cited by 97
Self-citation
Metabolic fate of saturated and monounsaturated dietary fats: The Mediterranean diet revisited from epidemiological evidence to cellular mechanisms
Bergouignan, A., Momken, I., Schoeller, D. A., Simon, C., & Blanc, S. (2009) · Progress in Lipid Research · doi:10.1016/j.plipres.2009.02.004 · Cited by 99
Regulation of hormone‐sensitive lipase activity and Ser563 and Ser565 phosphorylation in human skeletal muscle during exercise
Roepstorff, C., Vistisen, B., Donsmark, M., Nielsen, J. N., Galbo, H., Green, K. A., Hardie, D. G., Wojtaszewski, J. F. P., Richter, E. A., & Kiens, B. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2004.066480 · Cited by 94
Self-citation
Genetic impairment of AMPKα2 signaling does not reduce muscle glucose uptake during treadmill exercise in mice
Maarbjerg, S., Jørgensen, S. B., Rose, A. J., Jeppesen, J. F., Jensen, T. E., Treebak, J. T., Birk, J. B., Schjerling, P., Wojtaszewski, J. F. P., & Richter, E. A. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90653.2008 · Cited by 95
Self-citation
Effect of endurance exercise training on Ca 2+ –calmodulin‐dependent protein kinase II expression and signalling in skeletal muscle of humans
Rose, A. J., Frøsig, C., Kiens, B., Wojtaszewski, J. F. P., & Richter, E. A. (2007) · The Journal of Physiology · doi:10.1113/jphysiol.2007.138529 · Cited by 92
Self-citation
Age‐related anabolic resistance after endurance‐type exercise in healthy humans
Durham, W. J., Casperson, S. L., Dillon, E. L., Keske, M. A., Paddon‐Jones, D., Sanford, A. P., Hickner, R. C., Grady, J. J., & Sheffield‐Moore, M. (2010) · The FASEB Journal · doi:10.1096/fj.09-150177 · Cited by 93
Two weeks of metformin treatment induces AMPK-dependent enhancement of insulin-stimulated glucose uptake in mouse soleus muscle
Kristensen, J. M., Treebak, J. T., Schjerling, P., Goodyear, L. J., & Wojtaszewski, J. F. P. (2014) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00417.2013 · Cited by 89
Self-citation
Relative Workload Determines Exercise-Induced Increases in PGC-1α mRNA
Nordsborg, N. B., Lundby, C., Leick, L., & Pilegaard, H. (2010) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e3181d2d21c · Cited by 86
Role of 5′AMP‐activated protein kinase in glycogen synthase activity and glucose utilization: insights from patients with McArdle's disease
Nielsen, J. N., Wojtaszewski, J. F. P., Haller, R. G., Hardie, D. G., Kemp, B. E., Richter, E. A., & Vissing, J. (2002) · The Journal of Physiology · doi:10.1113/jphysiol.2002.018044 · Cited by 86
Self-citation
β‐adrenergic stimulation of skeletal muscle HSL can be overridden by AMPK signaling
Watt, M. J., Steinberg, G. R., Chan, S. M. H., GARNHAM, A. P., Kemp, B. E., & Febbraio, M. A. (2004) · The FASEB Journal · doi:10.1096/fj.03-1067fje · Cited by 83
Endurance Exercise Enhances the Effect of Strength Training on Muscle Fiber Size and Protein Expression of Akt and mTOR
Kazior, Z., Willis, S. J., Moberg, M., Apró, W., Calbet, J. A. L., Holmberg, H.-C., & Blomstrand, E. (2016) · PLOS ONE · doi:10.1371/journal.pone.0149082 · Cited by 82
SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion
Guerra, B., Guadalupe‐Grau, A., Fuentes, T., Ponce‐González, J. G., Morales‐Álamo, D., Olmedillas, H., Guillén, J. Á., Santana, A., & Calbet, J. A. L. (2010) · European Journal of Applied Physiology · doi:10.1007/s00421-010-1413-y · Cited by 81
AMPK activation increases uncoupling protein-3 expression and mitochondrial enzyme activities in rat muscle without fibre type transitions
Putman, C. T., Kiricsi, M., Pearcey, J., MacLean, I. M., Bamford, J. A., Murdoch, G. K., Dixon, W. T., & Pette, D. (2003) · The Journal of Physiology · doi:10.1113/jphysiol.2003.040691 · Cited by 110
5??? Adenosine Monophosphate-Activated Protein Kinase, Metabolism and Exercise
Aschenbach, W. G., Sakamoto, K., & Goodyear, L. J. (2004) · Sports Medicine · doi:10.2165/00007256-200434020-00003 · Cited by 80
LKB1 Regulates Lipid Oxidation During Exercise Independently of AMPK
Jeppesen, J. F., Maarbjerg, S., Jordy, A. B., Fritzen, A. M., Pehmøller, C. K., Sylow, L., Serup, A. K., Jessen, N., Thorsen, K., Prats, C., et al. (2013) · Diabetes · doi:10.2337/db12-1160 · Cited by 82
Self-citation
AMP‐activated protein kinase in contraction regulation of skeletal muscle metabolism: necessary and/or sufficient?
Jensen, T. E., Wojtaszewski, J. F. P., & Richter, E. A. (2009) · Acta Physiologica · doi:10.1111/j.1748-1716.2009.01979.x · Cited by 78
Self-citation
AMPK activity and isoform protein expression are similar in muscle of obese subjects with and without type 2 diabetes
Højlund, K., Mustard, K. J., Stæhr, P., Hardie, D. G., Beck‐Nielsen, H., Richter, E. A., & Wojtaszewski, J. F. P. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00326.2003 · Cited by 80
Self-citation
Muscle glycogen content and glucose uptake during exercise in humans: influence of prior exercise and dietary manipulation
Steensberg, A., van Hall, G., Keller, C., Osada, T., Schjerling, P., Pedersen, B. K., Saltin, B., & Febbraio, M. A. (2002) · The Journal of Physiology · doi:10.1113/jphysiol.2001.015594 · Cited by 78
Insulin resistance and fuel homeostasis: the role of AMP‐activated protein kinase
Hegarty, B. D., Turner, N. A., Cooney, G. J., & Kraegen, E. W. (2009) · Acta Physiologica · doi:10.1111/j.1748-1716.2009.01968.x · Cited by 78
Exercise-induced molecular mechanisms promoting glycogen supercompensation in human skeletal muscle
Hingst, J. R., Bruhn, L., Hansen, M. B., Rosschou, M. F., Birk, J. B., Fentz, J., Foretz, M., Viollet, B., Sakamoto, K., Færgeman, N. J., et al. (2018) · Molecular Metabolism · doi:10.1016/j.molmet.2018.07.001 · Cited by 80
Self-citation
Oral glucose ingestion attenuates exercise-induced activation of 5′-AMP-activated protein kinase in human skeletal muscle
Akerstrom, T. C. A., Birk, J. B., Klein, D. K., Erikstrup, C., Plomgaard, P., Pedersen, B. K., & Wojtaszewski, J. F. P. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.02.057 · Cited by 75
Self-citation
Regulation of hepatic and peripheral glucose disposal
Moore, M. C., Cherrington, A. D., & Wasserman, D. H. (2003) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/s1521-690x(03)00036-8 · Cited by 74
Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling
Apró, W., Moberg, M., Hamilton, D. L., Ekblom, B., van Hall, G., Holmberg, H.-C., & Blomstrand, E. (2015) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00486.2014 · Cited by 82
Minireview: Adenosine 5′-Monophosphate-Activated Protein Kinase Regulation of Fatty Acid Oxidation in Skeletal Muscle
Osler, M. E., & Zierath, J. R. (2007) · Endocrinology · doi:10.1210/en.2007-1441 · Cited by 73
Enhanced rates of muscle protein synthesis and elevated mTOR signalling following endurance exercise in human subjects: Protein synthesis after aerobic exercise
Mascher, H., Ekblom, B., Rooyackers, O., & Blomstrand, E. (2011) · Acta Physiologica · doi:10.1111/j.1748-1716.2011.02274.x · Cited by 71
AMPK signaling in skeletal muscle during exercise: Role of reactive oxygen and nitrogen species
Morales-Alamo, D., & Calbet, J. A. L. (2016) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.01.012 · Cited by 72
Nitric oxide increases cyclic GMP levels, AMP-activated protein kinase (AMPK)α1-specific activity and glucose transport in human skeletal muscle
Deshmukh, A. S., Long, Y. C., Barbosa, T. D. C., Karlsson, H., Glund, S., Zavadoski, W. J., Gibbs, E. M., Koistinen, H. A., Wallberg‐Henriksson, H., & Zierath, J. R. (2010) · Diabetologia · doi:10.1007/s00125-010-1716-x · Cited by 72
Signalling mechanisms in skeletal muscle: role in substrate selection and muscle adaptation
Hawley, J. A., Hargreaves, M., & Zierath, J. R. (2006) · Essays in Biochemistry · doi:10.1042/bse0420001 · Cited by 70
PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle
Brandt, N., Dethlefsen, M. M., Bangsbo, J., & Pilegaard, H. (2017) · PLoS ONE · doi:10.1371/journal.pone.0185993 · Cited by 71
Insulin signalling: effects of prior exercise
Wojtaszewski, J. F. P., Jørgensen, S. B., Frøsig, C., MacDonald, C., Birk, J. B., & Richter, E. A. (2003) · Acta Physiologica Scandinavica · doi:10.1046/j.1365-201x.2003.01151.x · Cited by 69
Self-citation
Acute and chronic treatment of ob/ob and db/db mice with AICAR decreases blood glucose concentrations
Halseth, A. E., Ensor, N. J., White, T., Ross, S. A., & Gulve, E. A. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)00557-0 · Cited by 68
Skeletal muscle glucose uptake during exercise: A focus on reactive oxygen species and nitric oxide signaling
Merry, T. L., & McConell, G. K. (2009) · IUBMB Life · doi:10.1002/iub.179 · Cited by 68
Regulatory mechanisms of skeletal muscle protein turnover during exercise
Rose, A. J., & Richter, E. A. (2008) · Journal of Applied Physiology · doi:10.1152/japplphysiol.91375.2008 · Cited by 67
Self-citation
What Should I Eat before Exercise? Pre-Exercise Nutrition and the Response to Endurance Exercise: Current Prospective and Future Directions
Rothschild, J. A., Kilding, A. E., & Plews, D. J. (2020) · Nutrients · doi:10.3390/nu12113473 · Cited by 74
The metabolic sensor AMPK: Twelve enzymes in one
Smiles, W. J., Ovens, A. J., Oakhill, J. S., & Kofler, B. (2024) · Molecular Metabolism · doi:10.1016/j.molmet.2024.102042 · Cited by 69
Tuber indicum polysaccharide relieves fatigue by regulating gut microbiota in mice
Zhong, L., Ma, N., Zheng, H., Ma, G., Zhao, L., & Hu, Q. (2019) · Journal of Functional Foods · doi:10.1016/j.jff.2019.103580 · Cited by 67
Intensified exercise training does not alter AMPK signaling in human skeletal muscle.
Clark, S., Chen, Z.-P., Murphy, K., Aughey, R., McKenna, M. J., Kemp, B., & Hawley, J. (2004) · American Journal of Physiology. Endocrinology and Metabolism · doi:10.1152/ajpendo.00462.2003 · Cited by 78
Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type
Rose, A. J., Bisiani, B., Vistisen, B., Kiens, B., & Richter, E. A. (2008) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.90806.2008 · Cited by 66
Self-citation
Exercise-Induced Protein Kinase C Isoform-Specific Activation in Human Skeletal Muscle
Perrini, S., Henriksson, J., Zierath, J. R., & Widegren, U. (2004) · Diabetes · doi:10.2337/diabetes.53.1.21 · Cited by 65
Caffeine activates preferentially α1-isoform of 5′AMP-activated protein kinase in rat skeletal muscle
Egawa, T., Hamada, T., Ma, X., Karaike, K., Kameda, N., Masuda, S., Iwanaka, N., & Hayashi, T. (2010) · Acta Physiologica · doi:10.1111/j.1748-1716.2010.02169.x · Cited by 63
Mammalian γ2 AMPK regulates intrinsic heart rate
Yavari, A., Bellahcene, M., Bucchi, A., Sirenko, S., Pintér, K., Herring, N., Jung, J., Tarasov, K. V., Sharpe, E. J., Wolfien, M., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-01342-5 · Cited by 61
Hormone-sensitive lipase activity and fatty acyl-CoA content in human skeletal muscle during prolonged exercise
Watt, M. J., Heigenhauser, G. J. F., O'Neill, M., & Spriet, L. L. (2003) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01181.2002 · Cited by 60
A comprehensive view of muscle glucose uptake: regulation by insulin, contractile activity, and exercise
Richter, E. A., Bilan, P. J., & Klip, A. (2025) · Physiological Reviews · doi:10.1152/physrev.00033.2024 · Cited by 64
Self-citation
Endothelial nitric oxide synthase, vascular integrity and human exceptional longevity
Puca, A. A., Carrizzo, A., Ferrario, A., Villa, F., & Vecchione, C. (2012) · Immunity & Ageing · doi:10.1186/1742-4933-9-26 · Cited by 59
What makes an endurance athlete world-class? Not simply a physiological conundrum
Myburgh, K. H. (2003) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/s1095-6433(03)00220-4 · Cited by 57
Insulin signalling downstream of protein kinase B is potentiated by 5′AMP-activated protein kinase in rat hearts in vivo
Longnus, S. L., Ségalen, C., Giudicelli, J., Sajan, M. P., Farese, R. V., & Van Obberghen, E. (2005) · Diabetologia · doi:10.1007/s00125-005-0016-3 · Cited by 58
The α-subunit of AMPK is essential for submaximal contraction-mediated glucose transport in skeletal muscle in vitro
Lefort, N., St.-Amand, E., Morasse, S., Côté, C. H., & Marette, A. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90362.2008 · Cited by 57
Nutritional strategies to support concurrent training
Pérez‐Schindler, J., Hamilton, D. L., Moore, D. R., Baar, K., & Philp, A. (2014) · European Journal of Sport Science · doi:10.1080/17461391.2014.950345 · Cited by 63
Isoform-Specific Regulation of 5′ AMP-Activated Protein Kinase in Skeletal Muscle From Obese Zucker (fa/fa) Rats in Response to Contraction
Barnes, B. R., Ryder, J. W., Steiler, T. L., Fryer, L. G. D., Carling, D., & Zierath, J. R. (2002) · Diabetes · doi:10.2337/diabetes.51.9.2703 · Cited by 56
A single bout of exercise at higher intensity enhances glucose effectiveness in sedentary men.
Hayashi, Y., Nagasaka, S., Takahashi, N., Kusaka, I., Ishibashi, S., Numao, S., Lee, D.-J., Taki, Y., Ogata, H., Tokuyama, K., et al. (2005) · Journal of Clinical Endocrinology and Metabolism · doi:10.1210/jc.2004-2092 · Cited by 59
Hyperglycaemia is associated with impaired muscle signalling and aerobic adaptation to exercise
MacDonald, T. L., Pattamaprapanont, P., Pathak, P., Fernandez, N., de Freitas, E. C., Hafida, S., Mitri, J., Britton, S. L., Koch, L. G., & Lessard, S. J. (2020) · Nature Metabolism · doi:10.1038/s42255-020-0240-7 · Cited by 56
Training Intensity Distribution, Training Volume, and Periodization Models in Elite Swimmers: A Systematic Review
González‐Ravé, J. M., Hermosilla-Perona, F., González‐Mohíno, F., Casado, A., & Pyne, D. B. (2021) · International Journal of Sports Physiology and Performance · doi:10.1123/ijspp.2020-0906 · Cited by 58
The effect of concurrent training organisation in youth elite soccer players
Enright, K. J., Morton, J. P., Iga, J., & Drust, B. (2015) · European Journal of Applied Physiology · doi:10.1007/s00421-015-3218-5 · Cited by 57
New Insights into Molecular Mechanisms Mediating Adaptation to Exercise; A Review Focusing on Mitochondrial Biogenesis, Mitochondrial Function, Mitophagy and Autophagy
Roberts, F. L., & Markby, G. R. (2021) · Cells · doi:10.3390/cells10102639 · Cited by 54
Time course of GLUT4 and AMPK protein expression in human skeletal muscle during one month of physical training
Langfort, J., Viese, M., Ploug, T., & Dela, F. (2003) · Scandinavian Journal of Medicine and Science in Sports · doi:10.1034/j.1600-0838.2003.20120.x · Cited by 54
AMPK as a mediator of tissue preservation: time for a shift in dogma?
Langer, H. T., Rohm, M., Goncalves, M. D., & Sylow, L. (2024) · Nature Reviews Endocrinology · doi:10.1038/s41574-024-00992-y · Cited by 55
Low-intensity contraction activates the α1-isoform of 5′-AMP-activated protein kinase in rat skeletal muscle
Toyoda, T., Tanaka, S., Ebihara, K., Masuzaki, H., Hosoda, K., Sato, K., Fushiki, T., Nakao, K., & Hayashi, T. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00395.2005 · Cited by 51
AMP kinase activation ameliorates insulin resistance induced by free fatty acids in rat skeletal muscle
Olsen, G. S., & Hansen, B. F. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00118.2002 · Cited by 51
Self-citation
Exercise signalling to glucose transport in skeletal muscle
Richter, E. A., Nielsen, J. N., Jørgensen, S. B., Frøsig, C., Birk, J. B., & Wojtaszewski, J. F. P. (2004) · Proceedings of The Nutrition Society · doi:10.1079/pns2004343 · Cited by 53
Self-citation
Sodium bicarbonate ingestion augments the increase in PGC-1α mRNA expression during recovery from intense interval exercise in human skeletal muscle
Percival, M. E., Martin, B. J., Gillen, J. B., Skelly, L. E., MacInnis, M. J., Green, A. E., Tarnopolsky, M. A., & Gibala, M. J. (2015) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00048.2015 · Cited by 50
Cafeteria diet-induced insulin resistance is not associated with decreased insulin signaling or AMPK activity and is alleviated by physical training in rats
Brandt, N., Bock, K. D., Richter, E. A., & Hespel, P. (2010) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00098.2010 · Cited by 50
Self-citation
Metformin improves atypical protein kinase C activation by insulin and phosphatidylinositol-3,4,5-(PO4)3 in muscle of diabetic subjects
Luna, V., Casauban, L., Sajan, M. P., Gomez-Daspet, J., Powe, J. L., Miura, A., Rivas, J., Standaert, M. L., & Farese, R. V. (2006) · Diabetologia · doi:10.1007/s00125-005-0112-4 · Cited by 50
The effect of exercise and insulin on AS160 phosphorylation and 14-3-3 binding capacity in human skeletal muscle
Howlett, K. F., Mathews, A., Garnham, A., & Sakamoto, K. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00542.2007 · Cited by 47
AMP-activated Protein Kinase
Viollet, B., & Foretz, M. (2016) · Proceedings of the Fourth International Symposium on Polarization Phenomena in Nuclear Reactions · doi:10.1007/978-3-319-43589-3 · Cited by 48
Constant-Moderate and High-Intensity Interval Training Have Differential Benefits on Insulin Sensitive Tissues in High-Fat Fed Mice
Martínez-Huenchullán, S., Ban, L. A., Luisa Fernanda Olaya, A., Maharjan, B. R., Williams, P. F., Tam, C. S., McLennan, S. V., & Twigg, S. M. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.00459 · Cited by 47
Carbohydrate ingestion does not alter skeletal muscle AMPK signaling during exercise in humans
Lee-Young, R. S., Palmer, M. J., Linden, K. C., LePlastrier, K., Canny, B. J., Hargreaves, M., Wadley, G. D., Kemp, B. E., & McConell, G. K. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00023.2006 · Cited by 45

Authors: Wojtaszewski, J. F. P., Nielsen, P., Hansen, B. F., Richter, E. A., & Kiens, B.
Affiliations: University of Copenhagen (DK)
Novo Nordisk (Denmark) (DK)
Corresponding author: Wojtaszewski, Jørgen F. P.
ORCID: Wojtaszewski, Jørgen F. P.
Richter, Erik A.
Kiens, Bente
Venue: The Journal of Physiology
Publisher: Wiley
Published: 2000-10
Volume: 528
Issue: 1
Pages: 221-226
ISSN: 0022-3751 (ISSN-L)
1469-7793
DOI: 10.1111/j.1469-7793.2000.t01-1-00221.x
PMC: PMC2270117
OpenAlex: W2140558936
Semantic Scholar: 4455ede831ee4c56d6d419343f5a067b2777281c
Cited by: 435
References: 25
Citations by year: 2026: 7 · 2025: 11 · 2024: 10 · 2023: 11 · 2022: 19 · 2021: 18 · 2020: 23 · 2019: 16 · 2018: 13 · 2017: 13 · 2016: 19 · 2015: 18 · 2014: 14 · 2013: 23 · 2012: 16
FWCI: 6.52
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: The Journal of Physiology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders: Sundhed og Sygdom, Det Frie Forskningsråd
Danmarks Grundforskningsfond
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
Keywords: AMPK, exercise intensity, skeletal muscle metabolism, vastus lateralis, post-exercise recovery, human exercise
MeSH terms: Adult, Enzyme Activation, Exercise Test, Physical Exertion, Glycogen, Humans, Isoenzymes, Male, Multienzyme Complexes, Protein Serine-Threonine Kinases, Muscle, Skeletal, AMP-Activated Protein Kinases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Isoform‐specific and exercise intensity‐dependent activation of 5′‐AMP‐activated protein kinase in human skeletal muscle

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks