MYELINATION IN RAT BRAIN: METHOD OF MYELIN ISOLATION1
Abstract
Abstract— A procedure is described for the preparation from rat brain of myelin having the same degree of purity at all ages. Such a procedure is essential for the study of myelin composition during development. Microsomal contamination was successfully eliminated by adjusting the method to maintain a constant amount of brain per unit volume in the initial density gradient step, and by including two osmotic shocks and two low‐speed centrifugation steps. Myelin prepared in this way from animals ranging from 15 days to 14months of age had a total ATPase activity of 0.3‐2.0 μmol of P i.h−1.mg−1 dry wt of myelin, representing 0.1‐1.2 per cent recovery of the total homogenate activity; a Na+, K+‐ ATPase activity of 0.1‐1.6 μfnol of P i.h−1.mg−1 dry wt, representing 0.04‐1.5 per cent recovery; a nucleic acid content of 0.2‐0.7 per cent of myelin dry wt, representing 0.2‐2.0 per cent recovery; and a ganglioside NANA content of 0.04‐0.07 per cent of myelin dry wt. representing 0.2‐4.6 per cent recovery. The myelin prepared from 20‐day animals had the highest content of the first three constituents; otherwise the values of the four constituents were relatively constant per unit weight of myelin. The amounts of nucleic acid and ganglioside recovered in the myelin fractions increased with increasing age and myelin yield.
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“Myelin is composed of 35% cholesterol, 25% phospholipids, and 40% sphingolipids [210,211,212].”
From Revisiting the Pathogenesis of X-Linked Adrenoleukodystrophy (2025) · cites this paper as [212]
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- Authors
- Norton, W. T., & Poduslo, S. E.
- Affiliations
- Albert Einstein College of Medicine (US)
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1973-10
- Volume
- 21
- Issue
- 4
- Pages
- 749-757
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2119216843
- Cited by
- 1,413
- References
- 0
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 6 · 2023: 7 · 2022: 7 · 2021: 14 · 2020: 14 · 2019: 16 · 2018: 19 · 2017: 16 · 2016: 17 · 2015: 16 · 2014: 28 · 2013: 14 · 2012: 17
- FWCI
- 13.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Keywords
- rat brain, density gradient centrifugation, osmotic shock, Na+/K+-ATPase, nucleic acid content, myelin composition, brain development
- MeSH terms
- Acetates, Adenosine Triphosphatases, Animals, Brain, Cell Fractionation, Centrifugation, Density Gradient, Enzyme Activation, Gangliosides, Myelin Sheath, Neuraminic Acids, Nucleic Acids, Osmotic Pressure, Potassium, Sodium, Sucrose, Ultracentrifugation, Rats
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