Testosterone synthesized in cultured human SZ95 sebocytes derives mainly from dehydroepiandrosterone
Open accessAbstract
Human sebaceous gland possesses all the steroidogenic enzymes required for androgen synthesis. It remains unclear whether the testosterone produced in situ mainly derives from circulating dehydroepiandrosterone (DHEA) or from de novo synthesis utilizing serum cholesterol. Using testosterone radioimmunoassay, we found that testosterone was barely detectable in the supernatant of cultured human SZ95 sebocytes when cholesterol was added alone, indicating a low basal expression of steroidogenic acute regulatory protein (StAR) in SZ95 cells. Human chorionic gonadotropin and fibroblast growth factor-9 were as potent as forskolin in activating StAR to enhance testosterone production, while interleukin-1 beta, dexamethasone, insulin and insulin-like growth factor-1 showed no stimulatory effect. A two-fold increase of testosterone production was observed in supplementation of DHEA as compared to pregnenolone, progesterone or 17 alpha-hydroxyprogesterone. Based on our findings, testosterone synthesized in cultured sebocytes derived mainly from DHEA and inhibition of 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase may be a new target of androgen suppression for acne treatment.
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Testosterone synthesis by SZ95 sebocytes in vitro derives mainly from DHEA.
“In support of the latter, T synthesis by SZ95 sebocytes in vitro derives mainly from DHEA [93].”
From Steroidogenesis in the skin: Implications for local immune functions (2013) · cites this paper as [93]
Adding pregnenolone, progesterone, and 17α-hydroxyprogesterone significantly raised testosterone levels in culture media, reflecting 3βHSD1 and 17βHSD3 activity in SZ95 sebocytes.
“Addition of pregnenolone, progesterone and 17α-hydroxyprogesterone led to a significant rise of T levels in culture media, reflecting the activity of 3βHSD1 and 17βHSD3 on SZ95 sebocytes [93].”
From Steroidogenesis in the skin: Implications for local immune functions (2013) · cites this paper as [93]
Studies with cultured sebocytes suggest that circulating DHEA may be the more important source of androgen precursors.
“In fact, studies with cultured sebocytes suggest that circulating DHEA may be the more important source of androgen precursors (Chen et al., 2010).”
From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Chen et al., 2010
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Steroidogenesis in the skin: Implications for local immune functions
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Self-citationIntracrine androgen biosynthesis, metabolism and action revisited
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“In fact, studies with cultured sebocytes suggest that circulating DHEAmay be themore important source of androgen precursors (Chen et al., 2010).”
The skin as an endocrine organ
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- Authors
- Chen, W., Tsai, S., Sheu, H., Tsai, J., & Zouboulis, C. C.
- Affiliations
- Chang Gung University (TW)
- National Cheng Kung University (TW)
- Städtisches Klinikum Dessau (DE)
- Corresponding author
- Tsai, Shaw‐Jenq
- Venue
- Experimental Dermatology
- Publisher
- Wiley
- Published
- 2010-04-12
- Volume
- 19
- Issue
- 5
- Pages
- 470-472
- ISSN
- 0906-6705 (ISSN-L)
- 1600-0625
- OpenAlex
- W1976319330
- Cited by
- 48
- References
- 16
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 2 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 9 · 2013: 5 · 2012: 4
- FWCI
- 5.84
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Experimental Dermatology (publishedVersion)
- PubMed (publishedVersion)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
- Funders
- National Science Council
- Chang Gung Medical Foundation
- Topics
- Health Sciences › Medicine › Dermatology › Acne and Rosacea Treatments and Effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- testosterone production, sebocytes, dehydroepiandrosterone, androgen production, steroidogenic acute regulatory protein, human chorionic gonadotropin, FGF9, 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, acne treatment, androgen deprivation therapy, cholesterol
- MeSH terms
- Cell Line, Transformed, Cholesterol, Cyclic CMP, Dehydroepiandrosterone, Dexamethasone, Female, Colforsin, Chorionic Gonadotropin, Humans, Hydroxycholesterols, Insulin, Insulin-Like Growth Factor I, Pregnenolone, Progesterone, Sebaceous Glands, Testosterone, 17-alpha-Hydroxyprogesterone, Fibroblast Growth Factor 9, Interleukin-1beta
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