All 4 Bile Salt Hydrolase Proteins Are Responsible for the Hydrolysis Activity in Lactobacillus plantarum ST‐III
Abstract
Abstract: In vertebrates, bile salt hydrolysis plays an essential role in fat metabolism. Bile salts are synthesized in the liver. And in the small intestine glycine and taurine are de‐conjugated from bile salts by the enzyme bile salt hydrolase (BSH) from intestinal microbes. However, the mechanism of bile salt hydrolysis in Lactobacillus plantarum is still ambiguous. Four predicted bile salt hydrolase ( bsh ) genes from L. plantarum ST‐III were cloned into Escherichia coli . The function of these genes was explored by overexpression. All 4 proteins that were studied showed activity against glycine‐ or taurine‐conjugated bile salts. Substrate preference was also observed in BSH proteins, especially for the enzyme BSH1, which had high hydrolysis activity for glycodeoxycholic acid. These results suggest that all 4 bile salt hydrolases may be responsible for the bile salt hydrolysis activity in L. plantarum ST‐III. Practical Application: Hypercholesterolemia is considered one of the major risk factors for coronary heart disease. More interest has focused on intestinal microbes because of their role in the decrease of serum cholesterol. BSH proteins play an important role in the reduction of cholesterol. This paper adds to a better understanding of BSH proteins of intestinal microbes. It gives a great hint that probiotics can be used to solve hypercholesterolemia one day.
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BSH activity is widely distributed among gastrointestinal microbes, including various Lactobacillaceae species.
“BSH activity is widely distributed among gastrointestinal microbes, including various Lactobacillaceae species [5–8], where the presence of multiple bsh orthologs reflects strain-specific adaptations into the intestinal environment [5, 8–10].”
From Bile Salt Hydrolase Activity in the Food-Derived Strain Levilactobacillus brevis M3R3: Genomic and Functional Characterization (2026) · cites this paper as [8]
The presence of multiple bsh orthologs reflects strain-specific adaptations to the intestinal environment.
“BSH activity is widely distributed among gastrointestinal microbes, including various Lactobacillaceae species [5–8], where the presence of multiple bsh orthologs reflects strain-specific adaptations into the intestinal environment [5, 8–10].”
From Bile Salt Hydrolase Activity in the Food-Derived Strain Levilactobacillus brevis M3R3: Genomic and Functional Characterization (2026) · cites this paper as [8]
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“BSH activity is widely distributed among gastrointestinal microbes, including various Lactobacillaceae species [5–8], where the presence of multiple bsh orthologs reflects strain-specific adaptations into the intestinal environment [5, 8–10].”
- Authors
- Ren, J., Sun, K., Wu, Z., Yao, J., & Guo, B.
- Affiliations
- Shanghai Ocean University (CN)
- Corresponding author
- Ren, Jing
- Sun, Kejie
- Wu, Zhengjun
- Yao, Jing
- Guo, Benheng
- Venue
- Journal of Food Science
- Publisher
- Wiley
- Published
- 2011-11
- Volume
- 76
- Issue
- 9
- Pages
- M622-8
- ISSN
- 0022-1147 (ISSN-L)
- 1750-3841
- OpenAlex
- W2042656295
- Cited by
- 70
- References
- 29
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 7 · 2021: 8 · 2020: 10 · 2019: 8 · 2018: 8 · 2017: 4 · 2016: 1 · 2015: 1 · 2014: 4 · 2013: 3 · 2012: 1
- FWCI
- 3.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Food Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Program of Shanghai Subject Chief Scientist
- National Key Research and Development Program of China
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- bile salt hydrolase, bile salt hydrolysis, glycodeoxycholic acid, substrate preference, hypercholesterolemia, serum cholesterol reduction, probiotics, gut microbiota, fat metabolism
- MeSH terms
- Amidohydrolases, Amino Acid Sequence, Bile Acids and Salts, Chromatography, High Pressure Liquid, Cloning, Molecular, Escherichia coli, Genes, Bacterial, Hydrolysis, Molecular Sequence Data, Gene Expression Regulation, Bacterial, Lactobacillus plantarum, Lactiplantibacillus plantarum
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