Cited by 40Cholinesterase and Neurodegenerative DiseasesMedicinal Plants and NeuroprotectionAlzheimer's disease research and treatments

Neuroprotective effects of quercetin produced by an endophytic fungus Nigrospora oryzae isolated from Tinospora cordifolia

Vig, Rajat, Bhadra, Fatima, Gupta, Sukesh Kumar, Sairam, Krishnamurthy, Vasundhara, Mondem·2022·Journal of Applied Microbiology

Abstract

Aims Alzheimer’s disease is considered one of the most prevalent neurodegenerative disorders and dementia is the core symptom of this disease. This study was aimed to test the bioactive compounds produced by endophytic fungus for the inhibition of acetylcholinesterase (AChE) activity and to identify the compound responsible for this activity. Methods and Results Endophytic fungi were isolated from the medicinal plant Tinospora cordifolia and screened for AChE inhibition and antioxidant activity. The extract of one of the isolates Nigrospora oryzae (GL15) showed maximum AChE inhibition as well as antioxidant activity. The compound responsible for AChE inhibition (fraction 3) was identified as quercetin based on UV, FTIR spectra, HPLC and ESI-MS analyses. Furthermore, the identification of quercetin in the extract of fraction 3 was confirmed by 1H NMR analysis. This extract showed anti-dementia-like activity in scopolamine (SCO) model. The minimal effective dose of the extract of fraction 3 modulated the SCO-provoked cognitive deficits such as impairments in spatial recognition memory and latency period in Y-maze test and passive avoidance test, respectively. The SCO-induced modulation in cholinergic pathway was ameliorated by the extract of N. oryzae in hippocampus, resulting in decrease in AChE activity and restoration of cytoarchitecture of hippocampus. Conclusions The bioactive compound quercetin produced by N. oryzae may cure the learning and memory shortfalls via AChE-mediated mechanism in experimental mice. Significance and Impact of the Study The endophytic fungus N. oryzae serves as a potential source for the bioactive compound quercetin, which plays an important role in the management of Alzheimer’s disease.

Integrity signals

Registry

Crossref · 10.1111/jam.15174

Cites

71 of 71 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Vig, R., Bhadra, F., Gupta, S. K., Sairam, K., & Vasundhara, M.
Affiliations
  • Thapar Institute of Engineering & Technology (IN)
  • Indian Institute of Technology BHU (IN)
  • Neurotherapeutics Laboratory Department of Pharmaceutical Engineering and Technology Indian Institute of Technology (Banaras Hindu University) Varanasi India
Corresponding author
Vasundhara, Mondem
Venue
Journal of Applied Microbiology
Published
2021-07-06
Volume
132
Issue
1
Pages
365-380
ISSN
  • 1364-5072 (ISSN-L)
  • 1365-2672
OpenAlex
W3167333801
Cited by
40
References
71
Citations by year
2026: 5 · 2025: 13 · 2024: 13 · 2023: 6 · 2022: 3
FWCI
3.21
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
quercetin, Nigrospora oryzae, endophytic fungi, Tinospora cordifolia, Alzheimer's disease, acetylcholinesterase inhibition, antioxidant activity, scopolamine-induced cognitive deficits, memory impairment, hippocampus, cholinergic pathways, neuroprotection
MeSH terms
Acetylcholinesterase, Animals, Ascomycota, Memory Disorders, Plant Extracts, Quercetin, Neuroprotective Agents, Maze Learning, Tinospora, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review