Neuroprotective effects of quercetin produced by an endophytic fungus Nigrospora oryzae isolated from Tinospora cordifolia
Abstract
Aims Alzheimer’s disease is considered one of the most prevalent neurodegenerative disorders and dementia is the core symptom of this disease. This study was aimed to test the bioactive compounds produced by endophytic fungus for the inhibition of acetylcholinesterase (AChE) activity and to identify the compound responsible for this activity. Methods and Results Endophytic fungi were isolated from the medicinal plant Tinospora cordifolia and screened for AChE inhibition and antioxidant activity. The extract of one of the isolates Nigrospora oryzae (GL15) showed maximum AChE inhibition as well as antioxidant activity. The compound responsible for AChE inhibition (fraction 3) was identified as quercetin based on UV, FTIR spectra, HPLC and ESI-MS analyses. Furthermore, the identification of quercetin in the extract of fraction 3 was confirmed by 1H NMR analysis. This extract showed anti-dementia-like activity in scopolamine (SCO) model. The minimal effective dose of the extract of fraction 3 modulated the SCO-provoked cognitive deficits such as impairments in spatial recognition memory and latency period in Y-maze test and passive avoidance test, respectively. The SCO-induced modulation in cholinergic pathway was ameliorated by the extract of N. oryzae in hippocampus, resulting in decrease in AChE activity and restoration of cytoarchitecture of hippocampus. Conclusions The bioactive compound quercetin produced by N. oryzae may cure the learning and memory shortfalls via AChE-mediated mechanism in experimental mice. Significance and Impact of the Study The endophytic fungus N. oryzae serves as a potential source for the bioactive compound quercetin, which plays an important role in the management of Alzheimer’s disease.
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Anti-dementia-like activity of Nigrospora oryzae extract is due to quercetin, which plays a crucial role in Alzheimer's disease management.
“Also, the findings suggested that the anti-dementia-like activity is due to the potential bioactive compound quercetin produced by Nigrospora oryzae, which performs a crucial role in the management of AD [136].”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [136]
Extract of Nigrospora oryzae modulates cholinergic pathway in scopolamine-induced dementia model, resulting in AChE inhibition and antioxidant activity.
“The extract showed modulation of the cholinergic pathway in the scopolamineinduced model of dementia, which resulted in AChE inhibition and antioxidant activity.”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [136]
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- Authors
- Vig, R., Bhadra, F., Gupta, S. K., Sairam, K., & Vasundhara, M.
- Affiliations
- Thapar Institute of Engineering & Technology (IN)
- Indian Institute of Technology BHU (IN)
- Neurotherapeutics Laboratory Department of Pharmaceutical Engineering and Technology Indian Institute of Technology (Banaras Hindu University) Varanasi India
- Corresponding author
- Vasundhara, Mondem
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2021-07-06
- Volume
- 132
- Issue
- 1
- Pages
- 365-380
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W3167333801
- Cited by
- 40
- References
- 71
- Citations by year
- 2026: 5 · 2025: 13 · 2024: 13 · 2023: 6 · 2022: 3
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Keywords
- quercetin, Nigrospora oryzae, endophytic fungi, Tinospora cordifolia, Alzheimer's disease, acetylcholinesterase inhibition, antioxidant activity, scopolamine-induced cognitive deficits, memory impairment, hippocampus, cholinergic pathways, neuroprotection
- MeSH terms
- Acetylcholinesterase, Animals, Ascomycota, Memory Disorders, Plant Extracts, Quercetin, Neuroprotective Agents, Maze Learning, Tinospora, Mice
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