Conditional ablation of vasopressin-synthesizing neurons in transgenic rats
Open accessAbstract
Vasopressin-synthesizing neurons are located in several brain regions, including the hypothalamic paraventricular nucleus (PVN), supraoptic nucleus (SON) and suprachiasmatic nucleus (SCN). Vasopressin has been shown to have various functions in the brain, including social recognition memory, stress responses, emotional behaviors and circadian rhythms. The precise physiological functions of vasopressin-synthesizing neurons in specific brain regions remain to be clarified. Conditional ablation of local vasopressin-synthesizing neurons may be a useful tool for investigation of the functions of vasopressin neurons in the regions. In the present study, we characterized a transgenic rat line that expresses a mutated human diphtheria toxin receptor under control of the vasopressin gene promoter. Under a condition of salt loading, which activates the vasopressin gene in the hypothalamic PVN and SON, transgenic rats were i.c.v. injected with diphtheria toxin. Intracerebroventricular administration of diphtheria toxin after salt loading depleted vasopressin-immunoreactive cells in the hypothalamic PVN and SON, but not in the SCN. The number of oxytocin-immunoreactive cells in the hypothalamus was not significantly changed. The rats that received i.c.v. diphtheria toxin after salt loading showed polydipsia and polyuria, which were rescued by peripheral administration of 1-deamino-8-d-arginine vasopressin via an osmotic mini-pump. Intrahypothalamic administration of diphtheria toxin in transgenic rats under a normal hydration condition reduced the number of vasopressin-immunoreactive neurons, but not the number of oxytocin-immunoreactive neurons. The transgenic rat model can be used for selective ablation of vasopressin-synthesizing neurons and may be useful for clarifying roles of vasopressin neurons at least in the hypothalamic PVN and SON in the rat.
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Ablation of vasopressin cells in the paraventricular and supraoptic nuclei did not affect social recognition.
“However, it is unlikely that just PVN projections contribute to AVP effects on social recognition as ablation of AVP cells in PVN and SON did not affect social recognition (52,107).”
From Oxytocin, Vasopressin, and Social Behavior: From Neural Circuits to Clinical Opportunities (2022) · cites this paper as [107]
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Oxytocin, Vasopressin, and Social Behavior: From Neural Circuits to Clinical Opportunities
Rigney, N., de Vries, G. J., Petrulis, A., & Young, L. J. (2022) · Endocrinology · doi:10.1210/endocr/bqac111 · Cited by 191 · see citing works
- Authors
- Watanabe, J., Takayanagi, Y., Yoshida, M., Hattori, T., Saito, M., Kohno, K., Kobayashi, E., & Onaka, T.
- Affiliations
- Jichi Medical University (JP)
- Nara Institute of Science and Technology (JP)
- Keio University (JP)
- Corresponding author
- Takayanagi, Yuki
- Yoshida, Masahide
- Onaka, Tatsushi
- ORCID
- Venue
- Journal of Neuroendocrinology
- Publisher
- Wiley
- Published
- 2021-11-08
- Volume
- 33
- Issue
- 12
- Pages
- e13057-e13057
- ISSN
- 0953-8194 (ISSN-L)
- 1365-2826
- PMC
- PMC9285515
- OpenAlex
- W3207050752
- Cited by
- 5
- References
- 31
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroendocrinology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (17K08573, 20K07278, 17K08574, 20K07264, 17H04026, 20H03419)
- Naito Foundation
- Takeda Science Foundation
- Urakami Foundation for Food and Food Culture Promotion
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Electrolyte and hormonal disorders
- Keywords
- vasopressin, diphtheria toxin receptor, transgenic rats, paraventricular nucleus, supraoptic nucleus, suprachiasmatic nucleus, salt loading, polydipsia, polyuria, oxytocin, hypothalamus
- MeSH terms
- Animals, Diphtheria Toxin, Male, Neurons, Paraventricular Hypothalamic Nucleus, Rats, Inbred Lew, Supraoptic Nucleus, Vasopressins, Apoptosis, Gene Deletion, Gene Transfer Techniques, Genes, Transgenic, Suicide, Rats, Rats, Transgenic, Heparin-binding EGF-like Growth Factor
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