Cited by 17Insect symbiosis and bacterial influencesParasitic Diseases Research and TreatmentVector-borne infectious diseases

Molecular detection of Wolbachia pipientis in natural populations of mosquito vectors of Dirofilaria immitis from continental Portugal: first detection in Culex theileri

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DE PINHO MIXÃO, V., MENDES, A. M., MAURÍCIO, I. L., CALADO, M. M., NOVO, M. T., BELO, S., ALMEIDA, A. P. G.·2016·Medical and Veterinary Entomology

Abstract

Wolbachia pipientis (Rickettsiales: Rickettsiaceae) protects mosquitoes from infections with arboviruses and parasites. However, the effect of its co-infection on vector competence for Dirofilaria immitis (Spirurida: Onchocercidae) in the wild has not been investigated. This study aimed to screen vectors of D. immitis for wPip, to characterize these, and to investigate a possible association between the occurrence of W. pipientis and that of the nematode. The presence of W. pipientis was assessed in the five mosquito potential vectors of D. immitis in Portugal. Polymerase chain reaction (PCR) products were sequenced, and wPip haplotypes were determined by PCR-restricted fragment length polymorphism (RFLP). Results showed that wPip was detected in 61.5% of Culex pipiens (Diptera: Culicidae) pools and 6.3% of Culex theileri pools. wPip 16s rRNA sequences found in Cx. theileri exactly match those from Cx. pipiens, confirming a mosquito origin, rather than a nematode origin, as some specimens were infected with D. immitis. Only wPip haplotype I was found. No association was found between the presence of wPip and D. immitis in mosquitoes and hence a role for this endosymbiont in influencing vectorial competence is yet to be identified. This study contributes to understanding of wPip distribution in mosquito populations and, to the best of the authors' knowledge, is the first report of natural infections by wPip in Cx. theileri.

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Crossref · 10.1111/mve.12179

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  • In Portugal, D. immitis larvae have been identified in new mosquito species Cx. pipiens s.l., Cx. theileri, Ae. caspius, An. maculipennis s.l., and Ae. dentritus s.l. in Coimbra, Santarém, and Setúbal.

    “In Portugal, the presence of D. immitis larvae were identified in new species of mosquitoes, such us Cx. pipiens s.l.; Cx. theileri, Ae. caspius, An. maculipennis s.l., and Ae. dentritus s.l. in Coimbra (centre), Santarém, and Setúbal (centre-southern) [145,146].”
  • There are only three studies that have focused on investigating simultaneous infection with native Wolbachia and Dirofilaria in mosquitoes from natural populations.

    “There are only three studies that have focused on investigating simultaneous infection with native Wolbachia and Dirofilaria in mosquitoes from natural populations [22, 23, 51].”

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Authors
DE PINHO MIXÃO, V., MENDES, A. M., MAURÍCIO, I. L., CALADO, M. M., NOVO, M. T., BELO, S., & ALMEIDA, A. P. G.
Affiliations
  • Global Health and Tropical Medicine, GHTM, UEI Medical Parasitology, Instituto de Higiene e Medicina Tropical, IHMT Universidade Nova de Lisboa, UNL Lisbon Portugal
  • Universidade Politécnica de Lisboa (PT)
  • Universidade Nova de Lisboa (PT)
  • University of Pretoria (ZA)
Corresponding author
ALMEIDA, A. P. G.
Venue
Medical and Veterinary Entomology
Publisher
Wiley
Published
2016-06-09
Volume
30
Issue
3
Pages
301-309
ISSN
  • 0269-283X (ISSN-L)
  • 1365-2915
OpenAlex
W2409934405
Cited by
17
References
40
Citations by year
2026: 1 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 4
FWCI
1.44
Type
journal article
Language
en
Open access
Locations
  • Medical and Veterinary Entomology (publishedVersion)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
Funders
  • Fundação para a Ciência e Tecnologia (PTDC/SAU-SAP/113523/2009)
  • Global Health and Tropical Medicine (UID/Multi/04413/2013)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect symbiosis and bacterial influences
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
Keywords
Wolbachia pipientis, Dirofilaria immitis, Culex theileri, Culex pipiens, mosquito vectors, vector competence, 16S rRNA, endosymbionts, natural infection, Portugal
MeSH terms
Mosquito Vectors, Animals, Culex, Dirofilaria immitis, Dirofilariasis, DNA, Bacterial, Haplotypes, Portugal, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S, Polymerase Chain Reaction, Wolbachia

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